TY - BOOK ID - 207588 TI - Molecular Diagnostic PCR Handbook AU - Viljoen, Gerrit J. AU - Nel, Louis H. AU - Crowther, John R. PY - 2005 SN - 1280283807 9786610283804 1402034040 1402034032 9048168562 9781402034039 PB - Dordrecht : Springer Netherlands : Imprint: Springer, DB - UniCat KW - Polymerase chain reaction. KW - Molecular diagnosis. KW - Diagnosis KW - Molecular diagnostics KW - Molecular biology KW - Chain reaction, Polymerase KW - PCR (Biochemistry) KW - Polymerization KW - DNA polymerases KW - Molecular aspects KW - Medicine. KW - Biomedicine general. KW - Clinical sciences KW - Medical profession KW - Human biology KW - Life sciences KW - Medical sciences KW - Pathology KW - Physicians KW - Health Workforce KW - Biomedicine, general. KW - Polymerase chain reaction KW - Molecular diagnosis KW - Polymerase Chain Reaction KW - Diagnostic Techniques and Procedures KW - 57.082 KW - 57.082 Laboratory and museum techniques and equipment for plant and animal specimens and collections KW - Laboratory and museum techniques and equipment for plant and animal specimens and collections KW - Diagnostic Technics and Procedures KW - Technics and Procedures, Diagnostic KW - Techniques and Procedures, Diagnostic KW - Diagnostic Testing KW - Testing, Diagnostic KW - Sensitivity and Specificity KW - methods UR - https://www.unicat.be/uniCat?func=search&query=sysid:207588 AB - PREFACE The Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture is involved in agricultural research and development and assists Member States of FAO and IAEA in improving strategies to ensure food security through the use of nuclear techniques and related biotechnologies, where such techniques have a valuable and often unique role. In particular, molecular diagnostic methods have rapidly evolved in the past twenty years, since the advent of the Polymerase Chain Reaction (PCR). They are used in a wide range of agricultural areas such as, improving soil and water management; producing better crop varieties; diagnosing plant and animal diseases; controlling insect pests and improving food quality and safety. The uses of nucleic acid-directed methods have increased significantly in the past five years and have made important contributions to disease control country programmes for improving national and international trade. These developments include the more routine use of PCR as a diagnostic tool in veterinary diagnostic laboratories. However, there are many problems associated with the transfer and particularly, the application of this technology. These include lack of consideration of: the establishment of quality-assured procedures, the required set-up of the laboratory and the proper training of staff. This can lead to a situation where results are not assured. This book gives a comprehensive account of the practical aspects of PCR and strong consideration is given to ensure its optimal use in a laboratory environment. This includes the setting-up of a PCR laboratory; Good Laboratory Practice and standardised of PCR protocols. ER -