TY - BOOK ID - 63975406 TI - Transgenesis Techniques : Principles and Protocols PY - 2009 SN - 1603270191 1603270183 PB - Totowa, NJ : Humana Press : Imprint: Humana, DB - UniCat KW - Genetic engineering. KW - Human genetics. KW - Animal genetics. KW - Transgenic organisms. KW - Genetic Engineering. KW - Human Genetics. KW - Animal Genetics and Genomics. KW - Transgenics. KW - Genetically engineered organisms KW - Genetically modified organisms KW - GEOs (Genetically engineered organisms) KW - GMOs (Genetically modified organisms) KW - Organisms KW - Genetic engineering KW - Genetics KW - Designed genetic change KW - Engineering, Genetic KW - Gene splicing KW - Genetic intervention KW - Genetic surgery KW - Genetic recombination KW - Biotechnology KW - Transgenic organisms KW - Heredity, Human KW - Human biology KW - Physical anthropology UR - https://www.unicat.be/uniCat?func=search&query=sysid:63975406 AB - One of the major challenges currently facing the scientific community is to understand the function of the multitude of protein-coding genes that were revealed when the human genome was fully sequenced. In Transgenesis Techniques: Principles and Protocols, Third Edition, experts in the field fully update and expand upon the previous edition in order to detail the transgenic techniques currently used to modify the genome. The volume explores classic procedures to genetically modify mice and other model organisms, as well as cutting-edge practices involving microinjection, site-specific recombination systems, cryopreservation, and many other topics. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include brief introductions to their subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, Transgenesis Techniques: Principles and Protocols, Third Edition is a comprehensive practical guide to the generation of transgenic animals and an invaluable source of information in any lab currently involved in transgenic techniques. ER -