TY - BOOK ID - 669858 TI - Molecular cloning : a laboratory manual. AU - Sambrook, Joseph AU - Green, Michael R. PY - 2012 SN - 9781936113422 9781936113415 1936113414 1936113422 PB - Cold Spring Harbor Cold Spring Harbor laboratory DB - UniCat KW - polymers KW - DNA (deoxyribonucleic acid) KW - moleculaire biologie KW - genetische manipulatie KW - klonen KW - Molecular biology KW - Chemical laboratory practice KW - polymeren KW - Macromolecules KW - Molecular cloning KW - Cloning, Molecular KW - Clonage moléculaire KW - Laboratory manuals. KW - Manuels de laboratoire KW - ADN KW - DNA KW - molecular cloning KW - genes KW - technique de laboratoire KW - laboratory techniques KW - Vecteur génétique KW - Genetic vectors KW - Plasmide KW - plasmids KW - PCR KW - Bioinformatique KW - Bioinformatics KW - Transfection KW - transfection KW - Expression des gènes KW - gene expression KW - Interaction génique KW - gene interaction KW - Cloning, Molecular. KW - Molecular Cloning KW - Cosmids KW - Cloning, Organism KW - DNA cloning KW - Gene cloning KW - Cloning KW - Genetic engineering KW - Molecular genetics KW - Clone cells KW - 542 KW - clonen KW - laboratoriumtechnieken KW - groene chemie KW - Clonage moléculaire KW - Klonen KW - Moleculaire genetica KW - Genetische manipulatie KW - DNA. KW - transfection. KW - bio-informatica KW - mutageniteit KW - oligonucleotides KW - microarrays KW - gisten KW - Monograph KW - Molecular cloning - Laboratory manuals UR - https://www.unicat.be/uniCat?func=search&query=sysid:669858 AB - Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential. ... The theoretical and historical underpinnings of techniques are prominent features of the presentation throughout, information that does much to help trouble-shoot experimental problems. For the fourth edition of this classic work, the content has been entirely recast to include nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories. Core chapters from the third edition have been revised to feature current strategies and approaches to the preparation and cloning of nucleic acids, gene transfer, and expression analysis. They are augmented by 12 new chapters which show how DNA, RNA, and proteins should be prepared, evaluated, and manipulated, and how data generation and analysis can be handled. The new content includes methods for studying interactions between cellular components, such as microarrays, next-generation sequencing technologies, RNA interference, and epigenetic analysis using DNA methylation techniques and chromatin immunoprecipitation. To make sense of the wealth of data produced by these techniques, a bioinformatics chapter describes the use of analytical tools for comparing sequences of genes and proteins and identifying common expression patterns among sets of genes. Building on thirty years of trust, reliability, and authority, the fourth edition of Molecular Cloning is the new gold standard--the one indispensable molecular biology laboratory manual and reference source. --Publisher description. ER -