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This book describes the use of stem cells and cancer stem cell generation in the inflammatory microenvironment (cancer-inducing niche) using induced pluripotent stem cells. It provides step-by-step techniques and manuals for studying stem cell and cancer stem cell generation with different applications in cancer research. The development of induced pluripotent stem cells has provided a new approach to studying cancer initiation by producing cancer stem cells without introducing mutations or foreign genes. The book features the research of the authors’ group, which was the first to generate cancer stem cells from stem cells in the presence of inflammatory conditions. The 20 chapters if this book cover topics such as generating pluripotent stem cells, converting normal stem cells to cancer stem cells, enriching, isolating and evaluating cancer stem cells. Methods for evaluating the characteristics of cancer stem cells and possible therapies against them are also discussed. The book provides easy-to-follow protocols that help researchers in the study of cancer stem cells. Illustrations help readers understand how the method of cancer stem cell generation can be applied as an essential method for assessing the carcinogenic potential of various non-mutagenic compounds. It will be a useful resource for graduate students, researchers, technicians, and physicians working in academic, hospital, and pharmaceutical settings. .

Neoplastic Stem Cells --- Neoplastic Colony-Forming Units --- Tumor Initiating Cells --- Cancer Stem Cells --- Colony-Forming Units, Neoplastic --- Stem Cells, Neoplastic --- Tumor Stem Cells --- Cancer Stem Cell --- Cell, Cancer Stem --- Cell, Neoplastic Stem --- Cell, Tumor Initiating --- Cell, Tumor Stem --- Cells, Cancer Stem --- Cells, Neoplastic Stem --- Cells, Tumor Initiating --- Cells, Tumor Stem --- Colony Forming Units, Neoplastic --- Colony-Forming Unit, Neoplastic --- Initiating Cell, Tumor --- Initiating Cells, Tumor --- Neoplastic Colony Forming Units --- Neoplastic Colony-Forming Unit --- Neoplastic Stem Cell --- Stem Cell, Cancer --- Stem Cell, Neoplastic --- Stem Cell, Tumor --- Stem Cells, Cancer --- Stem Cells, Tumor --- Tumor Initiating Cell --- Tumor Stem Cell --- Unit, Neoplastic Colony-Forming --- Units, Neoplastic Colony-Forming --- Cancer. --- Biology—Technique. --- Stem cells. --- Cancer Biology. --- Biological Techniques. --- Cancer Stem Cells. --- Stem Cell Biology. --- Cancers --- Carcinoma --- Malignancy (Cancer) --- Malignant tumors --- Tumors --- Colony-forming units (Cells) --- Mother cells --- Progenitor cells --- Cells

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

This book describes the use of stem cells and cancer stem cell generation in the inflammatory microenvironment (cancer-inducing niche) using induced pluripotent stem cells. It provides step-by-step techniques and manuals for studying stem cell and cancer stem cell generation with different applications in cancer research. The development of induced pluripotent stem cells has provided a new approach to studying cancer initiation by producing cancer stem cells without introducing mutations or foreign genes. The book features the research of the authors’ group, which was the first to generate cancer stem cells from stem cells in the presence of inflammatory conditions. The 20 chapters if this book cover topics such as generating pluripotent stem cells, converting normal stem cells to cancer stem cells, enriching, isolating and evaluating cancer stem cells. Methods for evaluating the characteristics of cancer stem cells and possible therapies against them are also discussed. The book provides easy-to-follow protocols that help researchers in the study of cancer stem cells. Illustrations help readers understand how the method of cancer stem cell generation can be applied as an essential method for assessing the carcinogenic potential of various non-mutagenic compounds. It will be a useful resource for graduate students, researchers, technicians, and physicians working in academic, hospital, and pharmaceutical settings. .

Biological techniques --- Histology. Cytology --- Oncology. Neoplasms --- Biotechnology --- biologische technieken --- oncologie --- biotechnologie --- stamceltransplantatie --- Cancer. --- Biology—Technique. --- Stem cells. --- Cancer Biology. --- Biological Techniques. --- Cancer Stem Cells. --- Stem Cell Biology.