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The past decade has witnessed a true revolution in our understanding of how RNA molecules can reduce or silence gene expression, a process that holds great promise in basic research and for antiviral and anticancer applications. In RNA Silencing: Methods and Protocols, many of the most notable and distinguished researchers in the field describe in step-by-step detail their best methods for the design, preparation, and use of RNAs to reduce gene expression in cells and organisms. The techniques range widely and include methods addressing the biochemical aspects of the silencing machinery, RNA silencing in non-mammalian organisms, and the in vivo delivery of siRNAs and silencing vectors. There are also techniques for designing, preparing, and using RNAs to silence gene expression, for fine-tuning regulation by targeting specific isoforms of a given gene, and for the study and use of microRNAs. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, RNA Silencing: Methods and Protocols offers today's laboratory investigators a host of optimized and validated techniques to study RNA silencing and translate its concepts into successful practical applications.

Gene expression regulation --- Gene silencing --- Micrornas --- Rna interference. --- Rna, small interfering --- Small interfering rna --- Genetics. --- Physiology. --- Small interfering RNA --- Gene inactivation --- Inactivation, Gene --- Silencing, Gene --- piRNA (Piwi-interacting RNA) --- Piwi-interacting RNA --- Piwi protein-interacting RNA --- rasiRNA (Repeat-associated small interfering RNA) --- Repeat-associated siRNA --- Repeat-associated small interfering RNA --- Scan RNA --- scnRNA (Small scan RNA) --- Short hairpin RNA --- Short interfering RNA --- shRNA (Short hairpin RNA) --- siRNA (Small interfering RNA) --- Small hairpin RNA --- Small scan RNA --- tasiRNA (Trans-acting small interfering RNA) --- Trans-acting siRNA --- Trans-acting small interfering RNA --- Genetic regulation --- Antisense RNA --- Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists

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This book offers an ideal introduction to the analysis of demographic data. Inside, readers of all quantitative skill levels will find the information they need to develop a solid understanding of the methods used to study human populations and how they change over time due to such factors as birth, death, and migration. The comprehensive, systematic coverage defines basic concepts and introduces data sources; champions the use of Lexis diagrams as a device for visualizing demographic measures; highlights the importance of making comparisons (whether over time or between populations at a point in time) that control for differences in population composition; describes approaches to analyzing mortality, fertility, and migration; and details approaches to the important field of population projection.  Throughout, the author makes the material accessible for readers through careful exposition, the use of examples, and other helpful features. This book's thorough coverage of basic concepts and principles lays a firm foundation for anyone contemplating undertaking demographic research, whether in a university setting or in a professional employment that takes on a demographic dimension requiring in-house training.

Social sciences. --- Demography. --- Social Sciences. --- Methodology of the Social Sciences. --- Historical demography --- Social sciences --- Population --- Vital statistics --- Methodology. --- Behavioral sciences --- Human sciences --- Sciences, Social --- Social science --- Social studies --- Civilization --- Population. --- Sociology --- Population and Demography. --- Sociological Methods. --- Human population --- Human populations --- Population growth --- Populations, Human --- Economics --- Human ecology --- Demography --- Malthusianism

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This book offers an ideal introduction to the analysis of demographic data. Inside, readers of all quantitative skill levels will find the information they need to develop a solid understanding of the methods used to study human populations and how they change over time due to such factors as birth, death, and migration. The comprehensive, systematic coverage defines basic concepts and introduces data sources; champions the use of Lexis diagrams as a device for visualizing demographic measures; highlights the importance of making comparisons (whether over time or between populations at a point in time) that control for differences in population composition; describes approaches to analyzing mortality, fertility, and migration; and details approaches to the important field of population projection. Throughout, the author makes the material accessible for readers through careful exposition, the use of examples, and other helpful features. This book's thorough coverage of basic concepts and principles lays a firm foundation for anyone contemplating undertaking demographic research, whether in a university setting or in a professional employment that takes on a demographic dimension requiring in-house training.

Social sciences (general) --- Demography --- demografie --- sociale wetenschappen --- methodologieën