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In Electron Microscopy Methods and Protocols, well-practiced experts describe in detail the key electron microscopy techniques used for examining cells, tissue, biological macromolecules, molecular structure, and their interactions. With emphasis on cryotechniques for quantitative biological X-ray microanalysis, the book also includes those methods that use antibodies to locate proteins within cells and that prepare and analyze nucleic acids, proteins, and protein-nucleic acid complexes. Numerous immunogold labeling techniques for precise ultrastructural localization, distribution, and quantitation of macromolecules in cryo-fixed or chemically-fixed cells are described in sufficient detail to provide practical insight into their advantages and limitations. Electron Microscopy Methods and Protocols offers both newcomers and established researchers wanting to expand their repertoire of cutting-edge electron microscopy techniques-each optimized for reproducibility and robust results-today's gold-standard laboratory manual.

Biochemistry. --- Biochemistry, general.