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This open access volume gathers a variety of models, delivery systems, and approaches that can be used to assess RNA technology for exploiting antisense as a therapeutic intervention. Beginning with a section on the design of antisense technology and their delivery, the book continues by covering model systems developed to evaluate efficacy, both in vivo and in vitro, as well as methods to evaluate preclinically the toxicity associated with these new potential drugs, and intellectual property considerations. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Antisense RNA Design, Delivery, and Analysis provides basic knowledge and a large collection of methods to facilitate the work of newcomers to this vibrant and expanding field. This book was conceived thanks to the network DARTER (Delivery of Antisense RNA Therapeutics). DARTER is funded by the EU Cooperation of Science and Technology (COST), which aims to enhance interaction and collaborations between researchers in Europe and other countries.
Therapeutics. --- Biomaterials. --- Nucleic acids. --- Nucleic Acid. --- Polynucleotides --- Biomolecules --- Medical treatment --- Therapy --- Treatment of diseases --- Treatments for diseases --- Clinical medicine --- RNA therapeutics --- Antisense technology --- Therapeutic design --- Oligonucleotides --- Model systems --- Oligonucleotide-mediated toxicology --- Open Access --- Àcids nuclèics --- RNA --- Teràpia genètica --- Teràpia gènica --- Terapèutica genètica --- Enginyeria genètica --- Terapèutica --- Àcid ribonucleic --- ARN --- Àcids nucleics --- Micro RNAs --- Biomolècules --- ADN --- Nucleoproteïnes --- Transformació genètica
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This volume presents a comprehensive collection of detailed state-of-the-art protocols for gapmer-mediated RNA knockdown from leaders in the field. Chapters detail a historical and contemporary perspective of RNase H-inducing antisense oligonucleotides called gapmers and gapmer-mediated RNA knockdown for basic research and therapies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Gapmers: Methods and Protocols aims to provide diverse applications of gapmers along with protocols that will assist readers in moving the frontier.
ADN --- Àcid desoxiribonucleic --- DNA --- Àcids nucleics --- ADN fòssil --- ADN recombinant --- Elements genètics mòbils --- Gens --- Medical genetics. --- Medical Genetics. --- Clinical genetics --- Diseases --- Heredity of disease --- Human genetics --- Medical sciences --- Pathology --- Genetic disorders --- Genetic aspects
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This second edition provides updated and expanded chapters covering a broad sampling of useful and current methods in the rapidly developing and expanding field of bioinformatics. Bioinformatics, Volume I: Data, Sequence Analysis, and Evolution, Second Edition is comprised of three sections: Data and Databases, Sequence Analysis, and Phylogenetics and Evolution. The first section details bioinformatics methodologies in the generation of sequence and structural data and its organization into conceptual categories, and databases to facilitate further analyses. The Sequence Analysis section describes the fundamental methodologies for processing the sequences of biological molecules: techniques that are used in almost every pipeline of bioinformatics analysis, particularly in the preliminary stages of such pipelines. Last but not least, the phylogenetics and evolution section deals with methodologies that compare biological sequences for the purpose of understanding how they evolved. As a volume in the highly successful Methods in Molecular Biology series, chapters feature the kind of detail and expert implementation advice to ensure positive results. Comprehensive and practical, Bioinformatics, Volume I: Data, Sequence Analysis, and Evolution, Second Edition is an essential resource for graduate students, early career researchers, and others who are in the process of integrating new bioinformatics methods into their research.
Bioinformatics. --- Bio-informatics --- Biological informatics --- Biology --- Information science --- Computational biology --- Systems biology --- Data processing --- Bioinformàtica --- Seqüència de nucleòtids --- Manuals de laboratori --- Informàtica biològica --- Ciències de la informació --- Biologia computacional --- Tècniques de laboratori --- Anàlisi seqüencial d'àcids nucleics --- Seqüència ADN --- Seqüència de l'ADN --- Seqüències de nucleòtids --- Seqüències dels nucleòtids --- Codi genètic
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This detailed volume examines bioinformatic and molecular biological methods useful to identify and to explore the functions of CpG islands, key navigation points to understand gene regulation in fundamental processes such as development and cell differentiation as well as in diseases like cancer. Beginning with a historical perspective and important properties of CpG islands, the book continues with sections on computational and wet lab methods related to the study of DNA methylation, and in-depth protocols for the analysis of CpG island functional features including epigenetic profiling and chromatin interactions. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, CpG Islands: Methods and Protocols aims to provide readers with the information and methodologies necessary to continue to decipher how a genome’s structure and organization contribute to regulate biological processes.
Human genetics. --- Human Genetics. --- Genetics --- Heredity, Human --- Human biology --- Physical anthropology --- ADN --- Metilació --- Bioinformàtica --- Manuals de laboratori --- Tècniques de laboratori --- Informàtica biològica --- Ciències de la informació --- Biologia computacional --- Àcid desoxiribonucleic --- DNA --- Àcids nucleics --- ADN fòssil --- ADN recombinant --- Elements genètics mòbils --- Gens
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This volume details practical procedures on the latest DNA vaccine technology. Chapters guide readers through methods and protocols on DNA vaccine design, the adjuvant influence, production and purification methodologies, delivery systems, and approaches of the influence of DNA vaccines in the immunological response performance and in the cancer immunotherapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, DNA Vaccines: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Vacunes --- ADN --- Àcid desoxiribonucleic --- DNA --- Àcids nucleics --- ADN fòssil --- ADN recombinant --- Elements genètics mòbils --- Gens --- Vacunació --- Vacunes antibacterianes --- Vacuna de la malària --- Vacunes antivíriques --- Immunitat --- Immunology. --- Medical genetics. --- Medical Genetics. --- Clinical genetics --- Diseases --- Heredity of disease --- Human genetics --- Medical sciences --- Pathology --- Genetic disorders --- Immunobiology --- Life sciences --- Serology --- Genetic aspects
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This book provides numerous methods for identification, validation, and functional characterization of chimeric RNAs, herein described as any transcript which contains the nucleotide sequence of two distinct parental genes. Beginning with identification and characterization through bioinformatics tools, the book continues with experimental validation using RT-PCR, hybridization methods for RNA, and the use of mass spectrometry data for fusion protein validation, as well as functional and mechanistic studies of chimeric RNAs and translational applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Chimeric RNA: Methods and Protocols is an ideal guide to this expansive field and an inspiration to researchers seeking to further our knowledge of these remarkable features of the transcriptome.
RNA --- Àcid ribonucleic --- ARN --- Àcids nucleics --- Micro RNAs --- Genetics. --- Medical genetics. --- Cancer. --- Genetics and Genomics. --- Medical Genetics. --- Cancer Biology. --- Cancers --- Carcinoma --- Malignancy (Cancer) --- Malignant tumors --- Tumors --- Clinical genetics --- Diseases --- Heredity of disease --- Human genetics --- Medical sciences --- Pathology --- Genetic disorders --- Biology --- Embryology --- Mendel's law --- Adaptation (Biology) --- Breeding --- Chromosomes --- Heredity --- Mutation (Biology) --- Variation (Biology) --- Genetic aspects
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This volume looks at the methodology and techniques used by experts to study how certain molecules function in specific locations, and their temporal patterns. Chapters in this book cover topics such as in vivo single-molecule tracking of voltage-gated calcium channels with split-fluorescent proteins in CRISPR-engineering C. elegans; protein-protein interactions in membranes using single particle tracking; neuropathological diseases revealed by quantum-dot single particle tracking; SPoD-OnSPAN; and investigating molecular diffusion inside small neuronal compartments with two-photon fluorescence correlation spectroscopy. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and comprehensive, Single Molecule Microscopy is a valuable resource for any researcher interested in learning more about this important field. .
Biomolècules --- Microscòpia mèdica --- Neurobiologia molecular --- Neurologia molecular --- Biologia molecular --- Neurobiologia --- Microscòpia clínica --- Microscopis en medicina --- Diagnòstic de laboratori --- Microscòpia --- Microcirurgia --- Molècules biològiques --- Molècules --- Àcids nucleics --- Biopolímers --- Metabòlits --- Proteïnes --- Neurosciences. --- Biophysics. --- Neuroscience. --- Bioanalysis and Bioimaging. --- Biological physics --- Biology --- Medical sciences --- Physics --- Neural sciences --- Neurological sciences --- Neuroscience --- Nervous system
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Bones --- Non-coding RNA. --- Diseases --- Genetic aspects. --- fRNA --- Functional RNA --- ncRNA --- nmRNA --- Non-messenger RNA --- Non-translated RNA --- Noncoding RNA --- Nontranslated RNA --- Small non-messenger RNA --- Small RNA --- snmRNA --- sRNA --- Untranslated RNA --- RNA --- Teixit ossi --- Ossos (Teixits) --- Teixits ossis --- Esquelet --- Teixit connectiu --- Calcificació --- Ossos --- Àcid ribonucleic --- ARN --- Àcids nucleics --- Micro RNAs
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RNA. --- DNA damage. --- Ribonucleic acid --- Ribose nucleic acid --- Nucleic acids --- Ribose --- Damage, DNA --- Biochemical genetics --- Mutation (Biology) --- ADN --- Reparació de l'ADN --- Biomolècules --- Procariotes --- Microorganismes --- Cianobacteris --- Molècules biològiques --- Molècules --- Àcids nucleics --- Biopolímers --- Metabòlits --- Proteïnes --- Biologia molecular --- Mecanismes de reparació de l'àcid desoxiribonucleic --- Reparació de l'àcid desoxiribonucleic --- Genètica bioquímica --- Medicaments antineoplàstics --- Àcid desoxiribonucleic --- DNA --- ADN fòssil --- ADN recombinant --- Elements genètics mòbils --- Gens
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This book presents the latest research on non-coding RNAs in cardiovascular disease, a major cause of death worldwide. Non-coding RNAs play a significant role in development, proliferation, differentiation and apoptosis. Since altered non-coding RNA expression is often associated with various diseases, their potential use in diagnostics, prognostics and therapeutics is an important current area of study. The book consists of six parts: 1) An overview of non-coding RNAs and cardiovascular system, 2) Bioinformatics and interactions, 3) Non-coding RNA regulation in cardiovascular system, 4) Non-coding RNAs and cardiovascular diseases, 5) Potential biomarkers and therapeutic implications, 6) Future prospects. It is particularly useful for researchers and students in the field of non-coding RNA and cardiovascular biology, as well as for cardiologists, pharmacologists and physiologists.
Molecular biology. --- Human genetics. --- Cardiology. --- Molecular Medicine. --- Human Genetics. --- Heart --- Internal medicine --- Genetics --- Heredity, Human --- Human biology --- Physical anthropology --- Molecular biochemistry --- Molecular biophysics --- Biochemistry --- Biophysics --- Biomolecules --- Systems biology --- Diseases --- Cardiovascular system. --- Circulatory system --- Vascular system --- Blood --- Circulation --- Malalties cardiovasculars --- RNA --- Àcid ribonucleic --- ARN --- Àcids nucleics --- Micro RNAs --- Malalties de l'aparell cardiovascular --- Malalties del sistema cardiovascular --- Malalties --- Hipotensió arterial --- Malalties coronàries --- Malalties del cor --- Trombosi --- Cardiologia
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