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This open access volume gathers a variety of models, delivery systems, and approaches that can be used to assess RNA technology for exploiting antisense as a therapeutic intervention. Beginning with a section on the design of antisense technology and their delivery, the book continues by covering model systems developed to evaluate efficacy, both in vivo and in vitro, as well as methods to evaluate preclinically the toxicity associated with these new potential drugs, and intellectual property considerations. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Antisense RNA Design, Delivery, and Analysis provides basic knowledge and a large collection of methods to facilitate the work of newcomers to this vibrant and expanding field. This book was conceived thanks to the network DARTER (Delivery of Antisense RNA Therapeutics). DARTER is funded by the EU Cooperation of Science and Technology (COST), which aims to enhance interaction and collaborations between researchers in Europe and other countries.

Therapeutics. --- Biomaterials. --- Nucleic acids. --- Nucleic Acid. --- Polynucleotides --- Biomolecules --- Medical treatment --- Therapy --- Treatment of diseases --- Treatments for diseases --- Clinical medicine --- RNA therapeutics --- Antisense technology --- Therapeutic design --- Oligonucleotides --- Model systems --- Oligonucleotide-mediated toxicology --- Open Access --- Àcids nuclèics --- RNA --- Teràpia genètica --- Teràpia gènica --- Terapèutica genètica --- Enginyeria genètica --- Terapèutica --- Àcid ribonucleic --- ARN --- Àcids nucleics --- Micro RNAs --- Biomolècules --- ADN --- Nucleoproteïnes --- Transformació genètica

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This second edition provides updated and expanded chapters covering a broad sampling of useful and current methods in the rapidly developing and expanding field of bioinformatics. Bioinformatics, Volume I: Data, Sequence Analysis, and Evolution, Second Edition is comprised of three sections: Data and Databases, Sequence Analysis, and Phylogenetics and Evolution. The first section details bioinformatics methodologies in the generation of sequence and structural data and its organization into conceptual categories, and databases to facilitate further analyses. The Sequence Analysis section describes the fundamental methodologies for processing the sequences of biological molecules: techniques that are used in almost every pipeline of bioinformatics analysis, particularly in the preliminary stages of such pipelines. Last but not least, the phylogenetics and evolution section deals with methodologies that compare biological sequences for the purpose of understanding how they evolved. As a volume in the highly successful Methods in Molecular Biology series, chapters feature the kind of detail and expert implementation advice to ensure positive results. Comprehensive and practical, Bioinformatics, Volume I: Data, Sequence Analysis, and Evolution, Second Edition is an essential resource for graduate students, early career researchers, and others who are in the process of integrating new bioinformatics methods into their research.

Bioinformatics. --- Bio-informatics --- Biological informatics --- Biology --- Information science --- Computational biology --- Systems biology --- Data processing --- Bioinformàtica --- Seqüència de nucleòtids --- Manuals de laboratori --- Informàtica biològica --- Ciències de la informació --- Biologia computacional --- Tècniques de laboratori --- Anàlisi seqüencial d'àcids nucleics --- Seqüència ADN --- Seqüència de l'ADN --- Seqüències de nucleòtids --- Seqüències dels nucleòtids --- Codi genètic

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This volume details practical procedures on the latest DNA vaccine technology. Chapters guide readers through methods and protocols on DNA vaccine design, the adjuvant influence, production and purification methodologies, delivery systems, and approaches of the influence of DNA vaccines in the immunological response performance and in the cancer immunotherapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, DNA Vaccines: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Vacunes --- ADN --- Àcid desoxiribonucleic --- DNA --- Àcids nucleics --- ADN fòssil --- ADN recombinant --- Elements genètics mòbils --- Gens --- Vacunació --- Vacunes antibacterianes --- Vacuna de la malària --- Vacunes antivíriques --- Immunitat --- Immunology. --- Medical genetics. --- Medical Genetics. --- Clinical genetics --- Diseases --- Heredity of disease --- Human genetics --- Medical sciences --- Pathology --- Genetic disorders --- Immunobiology --- Life sciences --- Serology --- Genetic aspects

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This volume looks at the methodology and techniques used by experts to study how certain molecules function in specific locations, and their temporal patterns. Chapters in this book cover topics such as in vivo single-molecule tracking of voltage-gated calcium channels with split-fluorescent proteins in CRISPR-engineering C. elegans; protein-protein interactions in membranes using single particle tracking; neuropathological diseases revealed by quantum-dot single particle tracking; SPoD-OnSPAN; and investigating molecular diffusion inside small neuronal compartments with two-photon fluorescence correlation spectroscopy. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and comprehensive, Single Molecule Microscopy is a valuable resource for any researcher interested in learning more about this important field. .

Biomolècules --- Microscòpia mèdica --- Neurobiologia molecular --- Neurologia molecular --- Biologia molecular --- Neurobiologia --- Microscòpia clínica --- Microscopis en medicina --- Diagnòstic de laboratori --- Microscòpia --- Microcirurgia --- Molècules biològiques --- Molècules --- Àcids nucleics --- Biopolímers --- Metabòlits --- Proteïnes --- Neurosciences. --- Biophysics. --- Neuroscience. --- Bioanalysis and Bioimaging. --- Biological physics --- Biology --- Medical sciences --- Physics --- Neural sciences --- Neurological sciences --- Neuroscience --- Nervous system

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RNA. --- DNA damage. --- Ribonucleic acid --- Ribose nucleic acid --- Nucleic acids --- Ribose --- Damage, DNA --- Biochemical genetics --- Mutation (Biology) --- ADN --- Reparació de l'ADN --- Biomolècules --- Procariotes --- Microorganismes --- Cianobacteris --- Molècules biològiques --- Molècules --- Àcids nucleics --- Biopolímers --- Metabòlits --- Proteïnes --- Biologia molecular --- Mecanismes de reparació de l'àcid desoxiribonucleic --- Reparació de l'àcid desoxiribonucleic --- Genètica bioquímica --- Medicaments antineoplàstics --- Àcid desoxiribonucleic --- DNA --- ADN fòssil --- ADN recombinant --- Elements genètics mòbils --- Gens