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Amino acid sequence.

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Peptides --- Amino Acid Sequence

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This volume surveys the current status of many of the important methods and approaches which are central to the study of protein structure and function. Many of the articles in this volume are written to emphasize the general utility of the method or approach which is at its core, and to provide sufficient literature references to enable the reader to adapt the method or approach to other applications. It is hoped that this volume will provide a source from which newcomers as well as experienced scient­ ists may becom& more familiar with recent developments and future trends in some of the important areas of protein research. The articles which comprise this book are selected proceedings from the Symposium of American Protein Chemists, which was held in San Diego, California, September 30 to October 3, 1985. The goal of the organizers of this first symposium was to provide a forum for discussion and inter­ action among scientists whose interests span the broad spectrum of protein structure and function research. The concept and timing of the symposium well received as evidenced by the approximately 500 delegates to the was symposium. The inaugural meeting was marked by a strong scientific pro­ gram with over 140 papers presented in either a lecture or poster format.

Proteins --- Amino acid sequence --- Analysis

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Genetic vectors --- Amino acid sequence

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Why a Second Edition? The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry. Key Features Highlights of the Second Edition: * Mass spectrophotometric characterization of proteins and peptides * N-terminal sequencing of proteins with blocked N termini * Internal amino acid sequence analysis after protease digestion in-gel and on-blot * Improved microscale peptide purification methods * Computer analysis of protein sequences * New protocols tested and refined through everyday use in authors' laboratories * Updated reference chapter covering all aspects of protein microsequencing

Amino acid sequence. --- Peptides --- Proteins --- Amino Acid Sequence. --- Amino acid sequence. --- Peptides --- Proteins --- Purification. --- Purification. --- Isolation & purification. --- Isolation & purification.