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Antisense nucleic acids --- Antisense peptides --- Antisense Elements (Genetics) --- DNA, Antisense --- Antisense-Nucleinsäuren. --- Zeitschrift. --- DNA, Antisense. --- Antisense nucleic acids. --- Antisense peptides. --- Antisense nucleotides --- Anti-Sense DNA --- Antisense DNA --- Anti Sense DNA --- DNA, Anti-Sense --- Anti-Sense Elements --- Anti-Sense Probes --- Antisense Probes --- Anti Sense Elements --- Anti Sense Probes --- Elements, Anti-Sense --- Probes, Anti-Sense --- Probes, Antisense --- Antisense proteins --- Antisense Elements (Genetics). --- Peptides --- Nucleic acids --- Antisense-Nucleinsäuren --- Zeitschrift --- Periodikum --- Zeitschriften --- Presse --- Fortlaufendes Sammelwerk --- Antisense-Nukleinsäuren --- Antisense-Nucleotidsequenz --- Basenkomplimentäre-Nucleotidsequenz --- Nucleinsäuren --- Molecular Biology. --- Biochemical Genetics --- Biology, Molecular --- Genetics, Biochemical --- Genetics, Molecular --- Molecular Genetics --- Biochemical Genetic --- Genetic, Biochemical --- Genetic, Molecular --- Molecular Genetic --- Genetic Phenomena --- Antisense-Nukleinsäuren --- Basenkomplimentäre-Nucleotidsequenz --- Nucleinsäuren

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MicroRNAs constitute a particularly important class of small RNAs given their abundance, broad phylogenetic conservation and strong regulatory effects, with plant miRNAs uniquely divulging their ancient evolutionary origins and their strong post-transcriptional regulatory effects. In Plant MicroRNAs: Methods and Protocols, experts in the field present chapters that focus on the identification, validation, and characterization of the miRNA class of RNAs, and address important aspects about heterochromatic small interfering RNAs. In addition, the methods contained in this volume emphasize miRNA analyses, but also include ways to distinguish one class of small RNAs from another. As a volume in the highly successful Methods in Molecular Biology™ series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Plant MicroRNAs: Methods and Protocols provides the research community with a set of protocols that will help advance vital miRNA research for all plant species, both in typical model species and non-model species alike.

Micrornas --- Molecular biology --- Plant molecular genetics --- Plants --- Rna, small interfering --- Small interfering rna. --- Methods --- Research --- Methodology. --- Genetics --- Small interfering RNA --- Petits ARN interférents --- RNA, Small Interfering --- MicroRNAs --- RNA, Antisense --- RNA, Small Untranslated --- Antisense Elements (Genetics) --- RNA, Untranslated --- Petits ARN interférents --- Noncoding RNA --- RNA, Non-Coding --- RNA, Non-Peptide-Coding --- RNA, Non-Protein-Coding --- RNA, Noncoding --- RNA, Nontranslated --- npcRNA --- Non-Coding RNA --- Non-Peptide-Coding RNA --- Non-Protein-Coding RNA --- Nontranslated RNA --- RNA, Non Coding --- RNA, Non Peptide Coding --- RNA, Non Protein Coding --- Untranslated RNA --- Anti-Sense Elements --- Anti-Sense Probes --- Antisense Probes --- Anti Sense Elements --- Anti Sense Probes --- Elements, Anti-Sense --- Probes, Anti-Sense --- Probes, Antisense --- Short Noncoding RNA --- Small Non-Coding RNA --- Small Non-Messenger RNA --- Small Non-Protein-Coding RNA --- Small Noncoding RNA --- Small Untranslated RNA --- sncRNA --- sncRNAs --- Non-Coding RNA, Small --- Non-Messenger RNA, Small --- Non-Protein-Coding RNA, Small --- Noncoding RNA, Short --- Noncoding RNA, Small --- RNA, Short Noncoding --- RNA, Small Non-Coding --- RNA, Small Non-Messenger --- RNA, Small Non-Protein-Coding --- RNA, Small Noncoding --- Small Non Coding RNA --- Small Non Messenger RNA --- Small Non Protein Coding RNA --- Untranslated RNA, Small --- Anti-Sense RNA --- Antisense RNA --- Anti Sense RNA --- RNA, Anti-Sense --- Plant --- Micro RNA --- MicroRNA --- Primary MicroRNA --- Primary miRNA --- miRNAs --- pre-miRNA --- pri-miRNA --- RNA, Small Temporal --- Small Temporal RNA --- miRNA --- stRNA --- MicroRNA, Primary --- RNA, Micro --- Temporal RNA, Small --- miRNA, Primary --- pre miRNA --- pri miRNA --- Methodological Studies --- Methodological Study --- Studies, Methodological --- Study, Methodological --- Method --- Short Hairpin RNA --- Short Interfering RNA --- Small Hairpin RNA --- Small Interfering RNA --- piRNA --- scnRNA --- shRNA --- tasiRNA --- Piwi-Interacting RNA --- RNA, Piwi-Interacting --- RNA, Scan --- Repeat-Associated siRNA --- Scan RNA --- Small Scan RNA --- Trans-Acting siRNA --- siRNA --- siRNA, Repeat-Associated --- siRNA, Trans-Acting --- Hairpin RNA, Short --- Hairpin RNA, Small --- Interfering RNA, Short --- Interfering RNA, Small --- Piwi Interacting RNA --- RNA, Piwi Interacting --- RNA, Short Hairpin --- RNA, Short Interfering --- RNA, Small Hairpin --- RNA, Small Scan --- Repeat Associated siRNA --- Scan RNA, Small --- Trans Acting siRNA --- siRNA, Repeat Associated --- siRNA, Trans Acting --- piRNA (Piwi-interacting RNA) --- Piwi-interacting RNA --- Piwi protein-interacting RNA --- rasiRNA (Repeat-associated small interfering RNA) --- Repeat-associated siRNA --- Repeat-associated small interfering RNA --- scnRNA (Small scan RNA) --- Short hairpin RNA --- Short interfering RNA --- shRNA (Short hairpin RNA) --- siRNA (Small interfering RNA) --- Small hairpin RNA --- Small scan RNA --- tasiRNA (Trans-acting small interfering RNA) --- Trans-acting siRNA --- Trans-acting small interfering RNA --- Methodology --- Biochemistry. --- Botany. --- Plant genetics. --- Medical genetics. --- Biochemistry, general. --- Plant Sciences. --- Plant Genetics and Genomics. --- Gene Function. --- Botanical science --- Phytobiology --- Phytography --- Phytology --- Plant biology --- Plant science --- Biology --- Natural history --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Chemistry --- Medical sciences --- Clinical genetics --- Diseases --- Heredity of disease --- Human genetics --- Pathology --- Genetic disorders --- Composition --- Genetic aspects --- Floristic botany

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RNA interference (RNAi), in which RNA silences RNA, is the most recent discovery to revolutionize the study of biology. In RNAi: Design and Applications, leaders in the field contribute state-of-the-art, easy to follow methods and bench protocols designed for practical, everyday use of RNAi in biological research. Divided into two parts, this comprehensive volume covers fundamentals including designs of RNAi, biochemical assay protocols for the major components of RNAi, and study of potential off-target effects, followed by an extensive section covering various applications of RNAi in diverse model organisms and systems, from antiviral and anticancer applications to altering flower color in plants. Cutting edge and clearly written, RNAi: Design and Applications enables a researcher with standard molecular biological training to perform major RNAi-related experiments and contribute to this revolutionary, growing field.

Gene silencing --- Genetic techniques --- Rna interference --- Rna, small interfering --- Small interfering rna --- Small interfering RNA --- Gene Silencing --- RNA, Small Interfering --- Genetic Techniques --- Laboratory Manuals --- RNA Interference --- RNA, Small Untranslated --- Investigative Techniques --- Epigenesis, Genetic --- RNA, Antisense --- Publication Formats --- Antisense Elements (Genetics) --- RNA, Untranslated --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Publication Characteristics --- Gene Expression Regulation --- RNA --- Nucleic Acids, Nucleotides, and Nucleosides --- Genetic Processes --- Nucleic Acids --- Chemicals and Drugs --- Genetic Phenomena --- Phenomena and Processes --- Animal Biochemistry --- Human Anatomy & Physiology --- Health & Biological Sciences --- Gene Products, RNA --- Non-Polyadenylated RNA --- RNA, Non-Polyadenylated --- Ribonucleic Acid --- Acid, Ribonucleic --- Non Polyadenylated RNA --- RNA Gene Products --- RNA, Non Polyadenylated --- Expression Regulation, Gene --- Regulation, Gene Action --- Regulation, Gene Expression --- Gene Action Regulation --- Regulation of Gene Expression --- Anti-Sense RNA --- Antisense RNA --- Anti Sense RNA --- RNA, Anti-Sense --- Epigenetic Process --- Epigenetics Processes --- Epigenetic Processes --- Genetic Epigenesis --- Process, Epigenetic --- Processes, Epigenetic --- Processes, Epigenetics --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Short Noncoding RNA --- Small Non-Coding RNA --- Small Non-Messenger RNA --- Small Non-Protein-Coding RNA --- Small Noncoding RNA --- Small Untranslated RNA --- sncRNA --- sncRNAs --- Non-Coding RNA, Small --- Non-Messenger RNA, Small --- Non-Protein-Coding RNA, Small --- Noncoding RNA, Short --- Noncoding RNA, Small --- RNA, Short Noncoding --- RNA, Small Non-Coding --- RNA, Small Non-Messenger --- RNA, Small Non-Protein-Coding --- RNA, Small Noncoding --- Small Non Coding RNA --- Small Non Messenger RNA --- Small Non Protein Coding RNA --- Untranslated RNA, Small --- Co-Suppression --- Cosuppression --- Posttranscriptional Gene Silencing --- RNA Silencing --- RNAi --- Gene Silencing, Post-Transcriptional --- Post-Transcriptional Gene Silencing --- Co Suppression --- Gene Silencing, Post Transcriptional --- Gene Silencing, Posttranscriptional --- Gene Silencings, Posttranscriptional --- Interference, RNA --- Post Transcriptional Gene Silencing --- Post-Transcriptional Gene Silencings --- Silencing, Post-Transcriptional Gene --- Genetic Technic --- Genetic Technics --- Genetic Technique --- Technic, Genetic --- Technics, Genetic --- Technique, Genetic --- Techniques, Genetic --- Genetics --- Short Hairpin RNA --- Short Interfering RNA --- Small Hairpin RNA --- Small Interfering RNA --- piRNA --- scnRNA --- shRNA --- tasiRNA --- Piwi-Interacting RNA --- RNA, Piwi-Interacting --- RNA, Scan --- Repeat-Associated siRNA --- Scan RNA --- Small Scan RNA --- Trans-Acting siRNA --- siRNA --- siRNA, Repeat-Associated --- siRNA, Trans-Acting --- Hairpin RNA, Short --- Hairpin RNA, Small --- Interfering RNA, Short --- Interfering RNA, Small --- Piwi Interacting RNA --- RNA, Piwi Interacting --- RNA, Short Hairpin --- RNA, Short Interfering --- RNA, Small Hairpin --- RNA, Small Scan --- Repeat Associated siRNA --- Scan RNA, Small --- Trans Acting siRNA --- siRNA, Repeat Associated --- siRNA, Trans Acting --- Gene Inactivation --- Inactivation, Gene --- Silencing, Gene --- piRNA (Piwi-interacting RNA) --- Piwi-interacting RNA --- Piwi protein-interacting RNA --- rasiRNA (Repeat-associated small interfering RNA) --- Repeat-associated siRNA --- Repeat-associated small interfering RNA --- scnRNA (Small scan RNA) --- Short hairpin RNA --- Short interfering RNA --- shRNA (Short hairpin RNA) --- siRNA (Small interfering RNA) --- Small hairpin RNA --- Small scan RNA --- tasiRNA (Trans-acting small interfering RNA) --- Trans-acting siRNA --- Trans-acting small interfering RNA --- Genetic Concepts --- Genetic Phenomenon --- Genetic Process --- Concept, Genetic --- Concepts, Genetic --- Genetic Concept --- Phenomena, Genetic --- Phenomenon, Genetic --- Process, Genetic --- Processes, Genetic --- Acids, Nucleic --- Noncoding RNA --- RNA, Non-Coding --- RNA, Non-Peptide-Coding --- RNA, Non-Protein-Coding --- RNA, Noncoding --- RNA, Nontranslated --- npcRNA --- Non-Coding RNA --- Non-Peptide-Coding RNA --- Non-Protein-Coding RNA --- Nontranslated RNA --- RNA, Non Coding --- RNA, Non Peptide Coding --- RNA, Non Protein Coding --- Untranslated RNA --- Anti-Sense Elements --- Anti-Sense Probes --- Antisense Probes --- Anti Sense Elements --- Anti Sense Probes --- Elements, Anti-Sense --- Probes, Anti-Sense --- Probes, Antisense --- methods --- Biochemistry. --- Human genetics. --- Medicine. --- Cytology. --- Biochemistry, general. --- Human Genetics. --- Molecular Medicine. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Health Workforce --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Chemistry --- Medical sciences --- Heredity, Human --- Human biology --- Physical anthropology --- Composition