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In the 1980s, capillary electrophoresis (CE) joined high-performance liquid chromatography (HPLC) as the most powerful separation technique available to analytical chemists and biochemists. Published research using CE grew from 48 papers in the year of commercial introduction (1988) to 1200 in 1997. While only a dozen major pharmaceutical and biotech companies have reduced CE to routine practice, the applications market is showing real or potential growth in key areas, particularly in the DNA marketplace for genomic mapping and forensic identification. For drug development involving small molecules (including chiral separations), one CE instrument can replace 10 liquid chromatographs in terms of speed of analysis. CE also uses aqueous rather than organic solvents and is thus environmentally friendlier than HPLC. The second edition of Practical Capillary Electrophoresis has been extensively reorganized and rewritten to reflect modern usage in the field, with an emphasis on commercially available apparatus and reagents. This authoritative and very comprehensible treatment builds on the author's extensive experience as an instructor of short courses for the American Chemical Society and for industry. Key Features * Illustrated with detailed diagrams of electrophoretic phenomena * Offers step-by-step methods development schemes * Presents techniques for developing quantitative, robust, and precise methods * Includes an extensive troubleshooting guide * Updates and greatly expands on the first edition-more than 50% of the text is new * Written by an internationally recognized scientist who is an instructor for American Chemical Society short courses on HPCE

Capillary electrophoresis --- Capillary gel electrophoresis --- Capillary zone electrophoresis --- Gel electrophoresis --- Zone electrophoresis --- Capillary electrophoresis.

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Capillaire elektroforese --- Elektroforese --- Analytische chemie --- Capillary electrophoresis --- Capillary gel electrophoresis --- Capillary zone electrophoresis --- Gel electrophoresis --- Zone electrophoresis

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The scientific monograph by the author Peter Mikus entitled ""Chiral Capillary Electrophoresis in Current Pharmaceutical and Biomedical Analysis"" provides a comprehensive view on the advanced capillary electrophoresis techniques aimed to current chiral bioanalysis. The advances in the chiral electrophoresis analytical approaches are divided and theoretically described in three sections involving (i) advanced chiral separations for the optimization of chiral resolution (separation mechanisms; electrophoresis techniques in capillary and microchip format; electrophoretic modes such as ITP, CZE/EKC, CEC; chiral additives / pseudophases / phases), (ii) advanced sample preparation for the on-line preconcentration, sample clean-up and analyte derivatization (implementation of electrophoretic effects such as stacking; non-electrophoretic effects such as SPE, chromatography, dialysis; combinations of these effects; multidimensional CE systems; instrumental schemes), (iii) advanced combinations of detection and electrophoresis for the optimization in qualitative and quantitative evaluation (the most important universal as well as selective detection approaches such as absorption and fluorescence spectrophotometry, electrochemical detection, mass spectrometry vs. (i) and/or (ii)). Real analytical potential (benefits and limitations) of these advanced analytical approaches is emphasized by selected performance parameters of the methods and illustrated by many current practical applications including chiral analyses of drugs, their (bio)degradation products and biomarkers in pharmaceutical and biological matrices. The author wishes the readers many inspirations in the creation of new innovative approaches in the field of advanced chiral electrophoresis techniques with the aim to overcome capabilities of the current analytical techniques.

Capillary electrophoresis. --- Chirality. --- Stereochemistry --- Symmetry (Physics) --- Enantiomers --- Capillary gel electrophoresis --- Capillary zone electrophoresis --- Gel electrophoresis --- Zone electrophoresis --- Toxicology and Pharmaceutical Science --- Pharmaceutical Science --- Pharmacology --- Pharmaceutical Chemistry --- Health Sciences

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This book is intended to be a working guide to the operation of capillary electrophoresis (CE) instrumentation. Since CE is still a rap­ idly maturing technique, detailed validated protocols are not widely established. Therefore, extensive experimental procedures are not pro­ vided for individual analyses. The intention is to provide general guide­ lines on the principles and practice of CE and to give an overview of the specific technologies and important application areas. Part I provides operating instructions for standard commercially available instruments. Guidelines are included for activities such as changing capillaries, method development, quantitative procedures, optimization of precision and sensitivity, and the validation of meth­ ods, fraction collection, and troubleshooting, as well as a quick guide to running a separation. The application range of CE is possibly the most diverse of all analytical techniques and ranges from large, complex macromolecules, such as proteins and nucleic acids, to small solutes, such as organic drugs and inorganic anions and cations.

Capillary electrophoresis. --- Microbiology. --- Microbial biology --- Capillary gel electrophoresis --- Capillary zone electrophoresis --- Biology --- Microorganisms --- Gel electrophoresis --- Zone electrophoresis --- Analytical biochemistry. --- Analytical Chemistry. --- Analytic biochemistry --- Biochemistry --- Chemistry, Analytic --- Bioanalytic chemistry --- Bioanalytical chemistry --- Analytical chemistry

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In Clinical Applications of Capillary Electrophoresis, Stephen Palfrey brings together for first time a collection of detailed capillary electrophoresis protocols designed exclusively for clinical applications. Written by the leading scientists who have often perfected these methods in their own laboratories, the protocols furnish new and more powerful assays for many routine serum and blood tests now regularly performed in clinical laboratories, including urine protein analysis, hemoglobin separation, and the detection of CSF proteins, lipoproteins, myoglobin, cryoglobulins, HbA1c, and cathepsin. The protocols offered for DNA studies include double-stranded DNA analysis, the prenatal diagnosis of Down's syndrome, Rh D/d genotyping, the identification of mutated p53 oncogene, and the detection of microsatellite instability in cancers. Many of the methods can be automated to replace the more costly and labor-intensive tests that are currently used in most clinical laboratories. Clinical Applications of Capillary Electrophoresis demonstrates clearly the simplicity, versatility, and power of CE over conventional methods. It offers to beginning clinical investigators, as well as established laboratories new to the technique, a representative range of highly practical CE methods-assays that are not only certain to become ever more productive, but are already eminently useful today.

Capillary electrophoresis --- Molecular diagnosis --- 543.2 --- 543.54 --- Capillaire elektroforese --- Diagnosis --- Molecular diagnostics --- Capillary gel electrophoresis --- Capillary zone electrophoresis --- Molecular aspects --- Molecular biology --- Gel electrophoresis --- Zone electrophoresis --- Pathology. --- Disease (Pathology) --- Medical sciences --- Diseases --- Medicine --- Medicine, Preventive