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Gel chromatography : theory, methodology, applications
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ISBN: 0471995487 9780471995487 Year: 1979 Publisher: Chichester John WIley & Sons

Aqueous size-exclusion chromatography
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ISBN: 0444429573 9786611781309 1281781304 0080858449 9780444429575 9780080858449 Year: 1988 Volume: 40 Publisher: Amsterdam New York New York, NY, U.S.A. Elsevier Distributors for the U.S. and Canada, Elsevier Science Pub. Co.

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Abstract

The rapid development of new packings for aqueous size-exclusion chromatography has revolutionized this field. High resolution non-adsorptive columns now make possible the efficient separation of proteins and the rapid and precise determination of the molecular weight distribution of synthetic polymers. This technology is also being applied to the separation of small ions, the characterization of associating systems, and the measurement of branching. At the same time, fundamental studies are elucidating the mechanisms of the various chromatographicprocesses.These developments in princi


Book
Gel permeation chromatography.
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ISBN: 0824710061 Year: 1971 Publisher: Dekker

Column handbook for size exclusion chromatography
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ISBN: 0127655557 9786611033811 1281033812 0080529399 9780127655550 9780080529394 9781281033819 Year: 1999 Publisher: San Diego, Calif. London Academic

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Column Handbook for Size Exclusion Chromatography is the first comprehensive reference to provide everything one needs to know about commercial analytical and preparative columns for size exclusion and gel filtration chromatography (SEC and GFC). SEC is now widely used as a quality assurance method in the polymer industry (both synthetic and biopolymers) to determine molecular weight and molecular weight distribution. The Handbook contains contributions from every column manufacturer around the world and from many experienced column users. It covers the technology, characterization, application, evaluation, maintenance, and quality control of analytical and preparative columns for SEC and GFC. Also included are columns for two closely related techniques, hydrodynamic chromatography and high osmotic pressure chromatography. Key Features * Evaluate and select columns with confidence for specific applications * Optimize separations and improve the ruggedness of analytical methods * Extend the service time of a column * Establish a quality-control program to ensure consistency in column performance * Avoid the expense of column damage or purchases that do not give the expected results

Size exclusion chromatography
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ISBN: 3540656359 3642084931 3662039109 9783540656357 9783642084935 Year: 1999 Publisher: Berlin Springer

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Although size exclusion chromatography (SEC) is perhaps the most popular and widely used technique for determining the molecular weight distribution of polymeric materials, there have been very few texts written on this topic. During the past decade, SEC has experienced a considerable amount of growth in regard to column and detector technology and new applications. With these advances, SEC can now be used for determining absolute molecular weight, polymer chain conformation and size, and branching, as well as polymer solution properties. This book introduces the reader to the fundamentals of SEC with emphasis on practical aspects of the technique, such as column and mobile selection, calibration, new detector capabilities and guidelines for performing SEC on most types of polymers, especially those of industrial importance. This book is intended for either those new to the field of SEC, or for those research workers who require a more comprehensive background.

Monoclonal antibody and immunosensor technology
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ISBN: 0444814132 9780080887357 008088735X 9780444814135 1281759775 0080858929 9786611759773 0444814124 0720442001 0444802231 0720442206 9780444814128 9780720442007 Year: 1991 Volume: 22 Publisher: Amsterdam New York New York, NY, U.S.A. Elsevier Sole distributors for the U.S.A. and Canada, Elsevier Science Pub. Co.

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This highly practical book, and successor to Volume 13 in the Laboratory Techniques series, explores further and provides more comprehensive, autoritative information on the production of Mabs. Much new and illuminating material has been included covering the concepts behind the application of recombinant DNA technology and biosensor technology to monoclonal antibodies, and all the human Mab technology facilitated by PCR of antibody genes. Also included in this latest volume is a section focussing on other methods of obtaining B cell clones such as short-term culture and oncogene tr

Keywords

Biological techniques --- Immunology. Immunopathology --- Biotechnology --- Monoclonal antibodies. --- Biosensors. --- Biotechnology. --- Chemical engineering --- Genetic engineering --- Biodetectors --- Biological detectors --- Biological sensors --- Biomedical detectors --- Biomedical sensors --- Detectors --- Medical instruments and apparatus --- Physiological apparatus --- Antibodies, Monoclonal --- Monoclonal immunoglobulins --- Immunoglobulins --- Molecular cloning --- Hybridomas --- Immunologic Techniques --- 57.083.3 --- 57.083.3 Immunological methods and techniques --- Immunological methods and techniques --- Antibody Dissociation --- Immunologic Technic --- Immunologic Technics --- Immunologic Technique --- Immunological Technics --- Immunological Techniques --- Technic, Immunologic --- Technics, Immunologic --- Technique, Immunologic --- Techniques, Immunologic --- Antibody Dissociations --- Dissociation, Antibody --- Dissociations, Antibody --- Immunological Technic --- Immunological Technique --- Technic, Immunological --- Technics, Immunological --- Technique, Immunological --- Techniques, Immunological --- Allergy and Immunology --- Immunity --- Immunochemistry --- Hybridoma --- Interleukin-6 --- Monoclonal Antibodies --- Monoclonal Antibody --- Antibody, Monoclonal --- methods --- Chromatography, Gel --- 543.544 --- 661.18 --- Biochemistry --- -Gel permeation chromatography --- Molecular biology --- -Molecular biochemistry --- Molecular biophysics --- Biophysics --- Biomolecules --- Systems biology --- Chromatography, Gel permeation --- Chromatography, Size exclusion --- Gel filtration chromatography --- SEC (Chromatographic analysis) --- Size exclusion chromatography --- Steric exclusion liquid chromatography --- Chromatographic analysis --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- 661.18 Colloids. Surface-active agents. Washing, foaming, flotation agents etc. --- Colloids. Surface-active agents. Washing, foaming, flotation agents etc. --- 543.544 Chromatographic analysis. Chromatography --- Chromatographic analysis. Chromatography --- Chromatography, Size Exclusion --- Exclusion Chromatography --- Gel Chromatography --- Gel Permeation Chromatography --- Molecular Sieve Chromatography --- Chromatography, Exclusion --- Chromatography, Gel Permeation --- Chromatography, Molecular Sieve --- Gel Filtration --- Gel Filtration Chromatography --- Chromatography, Gel Filtration --- Exclusion Chromatography, Size --- Filtration Chromatography, Gel --- Filtration, Gel --- Sieve Chromatography, Molecular --- Size Exclusion Chromatography --- Technique --- Composition --- -Technique --- Enzymology --- Clinical chemistry --- Fysicochemical separation methods --- Analytical biochemistry --- Histology. Cytology --- Radioimmunoassay.

Techniques of samples preparation for liquid scintillation counting
Author:
ISBN: 0444813047 9780080858913 0080858910 9780444813046 1281759740 9781281759740 0444813144 9780444813145 0720442001 0720442206 0444802231 9786611759742 Year: 1976

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Free radical species are generally short-lived due to their high reactivity and thus direct measurement and identification are often impossible. ESR is the only technique which has the potential for direct detection of radicals but in biological systems even these must be trapped by a spin-trapping agent. Thus most investigations involve recognition of indicators of the presence of radicals in vivo or ""FOOTPRINTS"" of radical-mediated damage. Techniques in Free Radical Research assembles and critically assesses the most relevant and reliable experimental approaches used towards t

Keywords

Analytical biochemistry --- Free Radicals - chemistry --- Free Radicals - adverse effects --- Research - methods --- Chromatography, Gel --- 543.544 --- 661.18 --- Biochemistry --- -Gel permeation chromatography --- Molecular biology --- -Molecular biochemistry --- Molecular biophysics --- Biophysics --- Biomolecules --- Systems biology --- Chromatography, Gel permeation --- Chromatography, Size exclusion --- Gel filtration chromatography --- SEC (Chromatographic analysis) --- Size exclusion chromatography --- Steric exclusion liquid chromatography --- Chromatographic analysis --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- 661.18 Colloids. Surface-active agents. Washing, foaming, flotation agents etc. --- Colloids. Surface-active agents. Washing, foaming, flotation agents etc. --- 543.544 Chromatographic analysis. Chromatography --- Chromatographic analysis. Chromatography --- Chromatography, Size Exclusion --- Exclusion Chromatography --- Gel Chromatography --- Gel Permeation Chromatography --- Molecular Sieve Chromatography --- Chromatography, Exclusion --- Chromatography, Gel Permeation --- Chromatography, Molecular Sieve --- Gel Filtration --- Gel Filtration Chromatography --- Chromatography, Gel Filtration --- Exclusion Chromatography, Size --- Filtration Chromatography, Gel --- Filtration, Gel --- Sieve Chromatography, Molecular --- Size Exclusion Chromatography --- Technique --- Composition --- Free radicals (Chemistry) --- Radicals (Chemistry) --- Free radical reactions --- Research --- Methodology. --- Free Radicals --- Research Design --- 541.515 --- 541.515 Free radicals --- Free radicals --- Data Adjustment --- Data Reporting --- Design, Experimental --- Designs, Experimental --- Error Sources --- Experimental Designs --- Matched Groups --- Methodology, Research --- Problem Formulation --- Research Methodology --- Research Proposal --- Research Strategy --- Research Technics --- Research Techniques --- Scoring Methods --- Experimental Design --- Adjustment, Data --- Adjustments, Data --- Data Adjustments --- Design, Research --- Designs, Research --- Error Source --- Formulation, Problem --- Formulations, Problem --- Group, Matched --- Groups, Matched --- Matched Group --- Method, Scoring --- Methods, Scoring --- Problem Formulations --- Proposal, Research --- Proposals, Research --- Reporting, Data --- Research Designs --- Research Proposals --- Research Strategies --- Research Technic --- Research Technique --- Scoring Method --- Source, Error --- Sources, Error --- Strategies, Research --- Strategy, Research --- Technic, Research --- Technics, Research --- Technique, Research --- Techniques, Research --- Clinical Trials Data Monitoring Committees --- adverse effects --- chemistry --- methods --- Radioimmunoassay. --- Biological techniques --- Enzymology --- Immunology. Immunopathology --- Clinical chemistry --- Fysicochemical separation methods --- Histology. Cytology --- -Technique

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