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Molecular cloning. --- Cloning, Molecular --- DNA cloning --- Gene cloning --- Cloning --- Genetic engineering --- Molecular genetics --- Clone cells --- Clonatge molecular
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Enginyeria genètica --- Intervenció genètica --- Manipulació genètica --- Recombinació genètica --- ADN recombinant --- Clonatge --- Clonatge molecular --- Enginyeria de proteïnes --- Enginyeria genètica animal --- Enginyeria genètica vegetal --- Teràpia genètica --- Fecundació in vitro --- Organismes transgènics
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A continuously evolving technique, immunotherapy for the treatment of cancers now incorporates the use of immune cells infused during bone marrow transplants as well as approaches like cell and gene therapy, while stem cell-based therapies, tissue engineering, and targeting have also contributed to the latest successes in pre-clinical immunotherapy studies. In Immunotherapy of Cancer: Methods and Protocols, expert researchers in the field describe detailed procedures for trainees and experts in the area of basic, clinical science who wish to undertake their own cutting-edge immunotherapy studies. In addition to the protocols, the volume also contains two general overviews providing useful updates in each area as well as summaries of recent pre-clinical and clinical trials. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Immunotherapy of Cancer: Methods and Protocols seeks to guide scientists along the path to a further developed system of immunotherapeutic treatments in order to reduce and hopefully eradicate this terrible disease.
Immunology. --- Oncology . --- Monoclonal antibodies. --- Oncology. --- Antibodies. --- Antibodies, Monoclonal --- Monoclonal immunoglobulins --- Immunoglobulins --- Molecular cloning --- Tumors --- Immunobiology --- Life sciences --- Serology --- Anticossos monoclonals --- Immunoteràpia --- Oncologia --- Medicina --- Oncologia geriàtrica --- Oncologia pediàtrica --- Oncologia veterinària --- Càncer --- Infermeria oncològica --- Terapèutica immunològica --- Immunologia clínica --- Terapèutica --- Adjuvants immunològics --- Seroteràpia --- Timosina --- Immunoglobulines monoclonals --- Clonatge molecular --- Immunoglobulines --- Rituximab
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This volume provides readers with a wide collection of the latest and readily reproducible technical protocols available in the field of non-viral gene delivery vectors. The chapters in this book are organized into three major parts: Part I is a section on conventional bolus gene delivery vectors that introduces typical transfection approaches relying on the addition of transfectants to the cell culture medium where the cells are grown in; Part II covers stimuli-responsive bolus transfectants and topics on gene delivery complexes made of smart polymers or stimuli-responsive polymers that change according to the environment they are in and delivered by dripping into cells; Part III discusses examples of substrate-mediated gene delivery—also termed reverse transfection—and the immobilization of a gene delivery vector onto a surface as opposed to more typical bolus delivery from the medium. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Non-Viral Gene Delivery Vectors: Methods and Protocols is written for experimentalists, and is an essential part of many laboratory bookshelves. This book will help novice and professionals alike succeed in their research in this field.
Human genetics. --- Human Genetics. --- Genetics --- Heredity, Human --- Human biology --- Physical anthropology --- Vectors genètics --- Manuals de laboratori --- Protocols clínics --- Plans d'actuació mèdica --- Protocols en medicina --- Protocols de diagnòstic clínic --- Protocols mèdics --- Protocols terapèutics --- Medicina clínica --- Històries clíniques --- Protocols d'infermeria --- Tècniques de laboratori --- Vectors de clonatge --- Clonatge molecular --- Plasmidis --- Virus --- ADN recombinant --- Expressió gènica
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This volume details generation of gene-edited cell lines and organisms as models for human diseases, pest control, and large animal welfare and production outcomes. Chapters guide readers through gene regulation, editing, screening of cell lines, genome editing, and an overview of the tools for efficient genome editing including; ZFNs, TALENs, and CRISPR. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step- by-step, readily reproducible protocols. Authoritative and cutting-edge, Applications of Genome Modulation and Editing aims to be a useful and practical guide for researchers to commence or advance their study in this field.
Genetics. --- Genetics and Genomics. --- Biology --- Embryology --- Mendel's law --- Adaptation (Biology) --- Breeding --- Chromosomes --- Heredity --- Mutation (Biology) --- Variation (Biology) --- Enginyeria genètica --- Regulació genètica --- Genomes --- Genoma --- Genoma humà --- Genòmica --- Biosíntesi --- Genètica molecular --- Inducció enzimàtica --- Expressió gènica --- Intervenció genètica --- Manipulació genètica --- Recombinació genètica --- ADN recombinant --- Clonatge --- Clonatge molecular --- Enginyeria de proteïnes --- Enginyeria genètica animal --- Enginyeria genètica vegetal --- Teràpia genètica --- Fecundació in vitro --- Organismes transgènics
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This detailed volume collects commonly used and cutting-edge methods to analyze alternative splicing, a key step in gene regulation. After an introduction of the alternative splicing mechanism and its targeting for therapeutic strategies, the book continues with techniques for analyzing alternative splicing profiles in complex biological systems, visualizing and localizing alternative spliced transcripts with cellular and sub-cellular resolution, probing regulators of alternative splicing, as well as assessing the functional consequences of alternative splicing. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Alternative Splicing: Methods and Protocols serves as an ideal guide for both RNA aficionados that want to implement novel approaches in their labs and novices undertaking alternative splicing projects.
Genetics. --- Genomics. --- Genetics and Genomics. --- Genome research --- Genomes --- Molecular genetics --- Biology --- Embryology --- Mendel's law --- Adaptation (Biology) --- Breeding --- Chromosomes --- Heredity --- Mutation (Biology) --- Variation (Biology) --- Research --- Enginyeria genètica --- Intervenció genètica --- Manipulació genètica --- Recombinació genètica --- ADN recombinant --- Clonatge --- Clonatge molecular --- Enginyeria de proteïnes --- Enginyeria genètica animal --- Enginyeria genètica vegetal --- Teràpia genètica --- Fecundació in vitro --- Organismes transgènics
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While the utilization of microarrays for gene expression studies has been widely published, Microarray Methods for Drug Discovery describes the use of this technology for a multitude of other vital applications. Covering the field of drug discovery through its various aspects, including high throughput screening, target identification, drug metabolism and toxicity screening as well as clinical sample handling, this volume also addresses new emerging fields like miRNA profiling, ribonomic and glycomic profiling. All chapters, contributed by experts in the field, follow the highly successful Methods in Molecular Biology™ series format, which is comprised of brief introductions, lists of required materials and reagents, readily reproducible, step-by-step lab protocols, along with detailed tips on troubleshooting and avoiding known pitfalls, making this book an easy read for investigators at all levels of expertise. Authoritative and cutting-edge, Microarray Methods for Drug Discovery provides an ideal desk reference for scientists who wish to use microarray technology in order to better understand a variety of biological interactions in their respective fields.
Toxicology. --- Human genetics. --- Pharmacology/Toxicology. --- Human Genetics. --- Chemicals --- Medicine --- Pharmacology --- Poisoning --- Poisons --- Genetics --- Heredity, Human --- Human biology --- Physical anthropology --- Toxicology --- Microarrays --- Genètica molecular --- Química farmacèutica --- Farmacoquímica --- Química dels medicaments --- Química mèdica --- Química patològica --- Química terapèutica --- Farmàcia --- Farmacologia --- Química --- Matèria mèdica --- Medicaments --- Química combinatòria --- Biologia molecular --- Genètica --- Clonatge molecular --- Codi genètic --- Elements genètics mòbils --- Genètica bioquímica --- Genètica molecular humana --- Genètica molecular vegetal --- Genòmica --- Gens --- Regulació genètica --- Variació en el nombre de còpies --- Bioxips --- Microarray
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This volume provides an introduction for the scientist who is new to the field of molecular imaging, as well as detailed methods for experts in other areas of molecular imaging. Chapters detail the advantages and limitations of combining fluorescent, bioluminescent and radioisotopic, creating dual modality imaging reporter gene construct, bioluminescent imaging, and bioluminescent or fluorescent imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Reporter Gene Imaging : Methods and Protocols aims to ensure successful results in the further study of this vital field.
Radiology, Medical. --- Imaging / Radiology. --- Clinical radiology --- Radiology, Medical --- Radiology (Medicine) --- Medical physics --- Gens --- Genètica molecular --- Radiologia mèdica --- Radiologia clínica --- Radiologia (Medicina) --- Física mèdica --- Medicina nuclear --- Radiofàrmacs --- Radiografia mèdica --- Radiologia dental --- Radiologia intervencionista --- Radiologia pediàtrica --- Radiologia veterinària --- Radioteràpia --- Diagnòstic radiològic --- Biologia molecular --- Genètica --- Clonatge molecular --- Codi genètic --- Elements genètics mòbils --- Genètica bioquímica --- Genètica molecular humana --- Genètica molecular vegetal --- Genòmica --- Regulació genètica --- Variació en el nombre de còpies --- Unitats d'informació genètica --- Herència (Biologia) --- ADN
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This book explores well-established and emerging conjugation strategies that are relevant for proteins used in the field of precision medicine, focusing on techniques that are suitable for antibodies, antibody-fragments such as Fabs, scFvs, or nanobodies, scaffold proteins such as FN3 or DARPin, peptides, or model proteins. Although centered on the development of bioconjugates rather than their application, most protocols also show the conjugation of the targeting vehicle to a diagnostic or therapeutic entity, with the end-product most often being an antibody-drug conjugate, an optical probe, a nanomedicine, or a radiopharmaceutical. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Bioconjugation: Methods and Protocols is an ideal guide for researchers looking toward precision medicine in order to expand the vital field of drug discovery.
Monoclonal antibodies. --- Biochemistry. --- Toxicology. --- Antibodies. --- Protein Science. --- Pharmacology/Toxicology. --- Chemicals --- Medicine --- Pharmacology --- Poisoning --- Poisons --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Antibodies, Monoclonal --- Monoclonal immunoglobulins --- Immunoglobulins --- Molecular cloning --- Toxicology --- Composition --- Anticossos monoclonals --- Toxicologia --- Manuals de laboratori --- Tècniques de laboratori --- Farmacologia --- Dermatotoxicologia --- Immunotoxicologia --- Neurotoxicologia --- Toxicologia ambiental --- Toxicologia experimental --- Toxicologia industrial --- Toxicologia genètica --- Toxicologia pediàtrica --- Emmetzinament --- Intoxicació --- Verins --- Immunoglobulines monoclonals --- Clonatge molecular --- Immunoglobulines --- Rituximab
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This volume provides a comprehensive collection of DNA assembly protocols that will prove useful for any researcher interested in molecular cloning, synthetic biology, or DNA manipulation. Chapters will guide readers through computational tools to design and track the construction of DNA assemblies, workflows that enable high-throughput assembly of DNA constructs, standardized toolkits and protocols for DNA assembly, and combinatorial solutions that enable the construction and optimization of entire metabolic pathways. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, DNA Cloning and Assembly: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Clonatge molecular --- Seqüència de nucleòtids --- Bioinformàtica --- Informàtica biològica --- Ciències de la informació --- Biologia computacional --- Anàlisi seqüencial d'àcids nucleics --- Seqüència ADN --- Seqüència de l'ADN --- Seqüències de nucleòtids --- Seqüències dels nucleòtids --- Codi genètic --- Clonació molecular --- Clonatge de molècules --- Clonatge de gens --- Clonatge d'ADN --- Clonatge --- Enginyeria genètica --- Genètica molecular --- Anticossos monoclonals --- Vectors genètics --- Medical genetics. --- Medical Genetics. --- Clinical genetics --- Diseases --- Heredity of disease --- Human genetics --- Medical sciences --- Pathology --- Genetic disorders --- Genetic aspects
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