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Chemical reactions. --- Chemistry, Organic. --- Combinatorial Chemistry Techniques. --- Reactions, Chemical --- Chemical processes

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Chemistry --- Combinatorial chemistry --- Molecular theory --- Chemistry. --- Combinatorial chemistry. --- Molecular theory. --- Combinatorial Chemistry Techniques. --- Chemistry.

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This book compiles key protocols instrumental to the study of high-throughput protein production and purification which have been refined and simplified over the years and are now ready to be transferred to any laboratory. Beginning with a section covering general procedures for high-throughput protein production, the volume continues with high-throughput protocols adapted to the production of specific protein families, as well as an extensive section on protocols combining high-throughput protein production and their micro-characterization. Written for the highly successful Methods in Molecular Biology series, chapters in this book include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, High-Throughput Protein Production and Purification: Methods and Protocols serves biochemists ranging from engineers, PhD students and post-doctoral fellows, to the heads of protein expression facilities and researchers, in pursuing this vital area of study.

Proteins --- Protein Biosynthesis. --- Combinatorial Chemistry Techniques. --- High-Throughput Screening Assays. --- Protéines --- Protéines --- Synthèse protéique. --- isolation & purification. --- Biotechnologie. --- Purification.

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Biological systems --- Combinatorial Chemistry Techniques. --- Combinatorial chemistry. --- Computational Biology --- Computational biology. --- Drug Design. --- Drug Evaluation, Preclinical --- Image Processing, Computer-Assisted --- Systems Biology --- Research --- Methodology. --- Methods. --- Methods. --- Methods. --- Methods.

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Descriptive organic chemistry --- Technology, Pharmaceutical --- Combinatorial Chemistry Techniques --- Gene Library --- Solid-phase synthesis --- Combinatorial chemistry --- 541.5 --- 615.015.2 --- #WSCH:LOSA --- Organic compounds --- Combinatorial organic synthesis --- Combinatorial synthesis (Organic chemistry) --- High-throughput organic synthesis --- High-throughput synthesis (Organic chemistry) --- Multiple parallel synthesis (Organic chemistry) --- Pharmaceutical chemistry --- Biochips --- Valencies. Bonds. Affinity --- Combined effects. Action of combination of drugs --- Synthesis --- Combinatorial chemistry. --- Solid-phase synthesis. --- 615.015.2 Combined effects. Action of combination of drugs --- 541.5 Valencies. Bonds. Affinity