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Histology. Cytology --- Microbial biotechnology --- Recombinant proteins --- Cells --- Recombinant Proteins --- Cell Culture --- Genetics, Microbial --- Biotechnology --- Biotechnologie microbienne --- Protéines recombinantes --- Periodicals --- Synthesis --- microbiology --- Periodicals. --- utilization --- Périodiques --- Synthèse --- Microbial biotechnology. --- Synthesis. --- Recombinant Proteins. --- Culture Techniques --- Genetics, Microbial. --- Biotechnology. --- microbiology. --- utilization. --- Microbial Genetics --- Genetic, Microbial --- Microbial Genetic --- Proteins, Biosynthetic --- Proteins, Recombinant DNA --- Biosynthetic Proteins --- DNA Recombinant Proteins --- DNA Proteins, Recombinant --- Proteins, DNA Recombinant --- Proteins, Recombinant --- Recombinant DNA Proteins --- Recombinant Proteins, DNA --- Genetically engineered proteins --- Proteins --- Microorganisms --- Biotechnologies --- Genetic engineering --- applied microbiology --- Virus Physiological Phenomena --- Bacterial Physiological Phenomena --- Genome, Microbial --- Bioreactors --- Recombinant molecules --- Industrial microbiology --- Biotechnological microorganisms --- Histology. --- Microbiology. --- Culture Technique --- Technique, Culture --- Techniques, Culture --- Diffusion Chambers, Culture --- General microbiology --- statistics & numerical data. --- Bioengineering --- Biosynthetic Protein --- Recombinant Protein --- Protein, Biosynthetic --- Protein, Recombinant

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Interferon --- Genetic engineering --- Recombinant proteins --- Genetic Engineering. --- Interferons. --- Recombinant Proteins. --- Genetic engineering. --- Interferon. --- Recombinant proteins. --- Agriculture Sciences --- Chemistry --- Engineering --- Life Sciences --- Agricultural Biotechnology --- Soil Chemistry, Microbiology, Fertility & Fertilizers --- Biochemistry --- Biotechnology --- biotechnology --- biomedicine --- biosciences --- microbiology --- molecular genetics --- immunology --- Genetically engineered proteins --- Proteins --- Interferons --- Designed genetic change --- Engineering, Genetic --- Gene splicing --- Genetic intervention --- Genetic surgery --- Proteins, Biosynthetic --- Proteins, Recombinant DNA --- Biosynthetic Proteins --- DNA Recombinant Proteins --- DNA Proteins, Recombinant --- Proteins, DNA Recombinant --- Proteins, Recombinant --- Recombinant DNA Proteins --- Recombinant Proteins, DNA --- Intervention, Genetic --- Genetic Intervention --- Genetic Interventions --- Interventions, Genetic --- Recombinant molecules --- Antineoplastic agents --- Antiviral agents --- Glycoproteins --- Lymphokines --- Genetic recombination --- Transgenic organisms --- Bioreactors --- Viral Interference --- Cloning, Molecular --- DNA, Recombinant --- Industrial Microbiology --- Artificial Gene Fusion --- Organisms, Genetically Modified --- Animals, Genetically Modified --- Plants, Genetically Modified --- biotechnologie --- Biosynthetic Protein --- Recombinant Protein --- Protein, Biosynthetic --- Protein, Recombinant

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In the last few years, significant advances have been made in understanding how a yeast cell responds to the stress of producing a recombinant protein, and how this information can be used to engineer improved host strains. The molecular biology of the expression vector, through the choice of promoter, tag and codon optimization of the target gene, is also a key determinant of a high-yielding protein production experiment. Recombinant Protein Production in Yeast: Methods and Protocols examines the process of preparation of expression vectors, transformation to generate high-yielding clones, optimization of experimental conditions to maximize yields, scale-up to bioreactor formats and disruption of yeast cells to enable the isolation of the recombinant protein prior to purification. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Recombinant Protein Production in Yeast: Methods and Protocols, seeks to aid scientists in adopting yeast as a protein production host.

Pichia --- Recombinant Proteins --- Recombinant proteins --- Saccharomyces cerevisiae --- Yeast fungi --- Yeasts --- biosynthesis --- Biotechnology --- Fungi --- Proteins --- Publication Formats --- Saccharomyces --- Saccharomycetales --- Publication Characteristics --- Ascomycota --- Eukaryota --- Amino Acids, Peptides, and Proteins --- Chemicals and Drugs --- Organisms --- Laboratory Manuals --- Health & Biological Sciences --- Biomedical Engineering --- Baker Yeast --- S cerevisiae --- Baker's Yeast --- Brewer's Yeast --- Yeast, Baker's --- Yeast, Brewer's --- Baker Yeasts --- Baker's Yeasts --- Bakers Yeast --- Brewer Yeast --- Brewer's Yeasts --- Brewers Yeast --- Yeast, Baker --- Yeast, Bakers --- Yeast, Brewer --- Yeast, Brewers --- Yeasts, Baker --- Yeasts, Baker's --- Yeasts, Brewer's --- Proteins, Biosynthetic --- Proteins, Recombinant DNA --- Biosynthetic Proteins --- DNA Recombinant Proteins --- DNA Proteins, Recombinant --- Proteins, DNA Recombinant --- Proteins, Recombinant --- Recombinant DNA Proteins --- Recombinant Proteins, DNA --- Hansenula --- Hansenulas --- Pichias --- Yeast --- Genetically engineered proteins --- Eucarya --- Eukarya --- Eukaryotes --- Eukaryotas --- Eukaryote --- Ascomycetes --- Cochliobolus --- Sclerotinia --- Ascomycete --- Ascomycotas --- Sclerotinias --- Budding Yeast --- Endomycetales --- Endomycopsis --- Yeast, Budding --- Budding Yeasts --- Endomycetale --- Endomycopses --- Saccharomycetale --- Yeasts, Budding --- Gene Products, Protein --- Gene Proteins --- Protein Gene Products --- Proteins, Gene --- Fungi, Filamentous --- Molds --- Filamentous Fungi --- Filamentous Fungus --- Fungus --- Fungus, Filamentous --- Mold --- Genetic engineering --- Saccharomyce --- Medicine. --- Human genetics. --- Biomedicine. --- Human Genetics. --- Genetics --- Heredity, Human --- Human biology --- Physical anthropology --- Clinical sciences --- Medical profession --- Life sciences --- Medical sciences --- Pathology --- Physicians

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She examines the decision-making processes at Monsanto that led to their making the drug available and discusses corporate, academic, and regulatory decision-making in the context of a restructured global political economy for agriculture. Mills shows that there was consensus about the scientific evidence but interpretation of that evidence differed depending on the context from which it was viewed. Scientists who analysed it for regulatory bodies interpreted it differently than scientists in corporate or academic institutions, and scientists in Canada and Europe interpreted it differently than those in the United States. In the United States it was assumed that any problems arising from its use could be taken care of within the existing dairy system; in Canada and Europe these problems were regarded as legitimate animal welfare issues. While all regulatory bodies agreed that human health problems were unlikely, in Canada the Health Protection Branch questioned this, but ultimately rejected the drug on animal health grounds.

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--- Social Protection --- Social Policy --- Action, Affirmative --- Migration Policies --- Policies, Migration --- Policies, Population --- Policies, Public --- Policies, Social --- Policy, Migration --- Policy, Population --- Policy, Public --- Policy, Social --- Population Policies --- Protection, Social --- Public Policies --- Social Policies --- Policy Making --- Adenohypophyseal Hormones --- Anterior Pituitary Hormones --- Hormones, Adenohypophyseal --- Hormones, Anterior Pituitary --- Agricultural Development --- Farming --- Development, Agricultural --- Agricultural Inoculants --- Gene Products, Protein --- Gene Proteins --- Protein --- Protein Gene Products --- Proteins, Gene --- Molecular Mechanisms of Pharmacological Action --- Proteins, Biosynthetic --- Proteins, Recombinant DNA --- Biosynthetic Protein --- Biosynthetic Proteins --- DNA Recombinant Proteins --- Recombinant Protein --- DNA Proteins, Recombinant --- Protein, Biosynthetic --- Protein, Recombinant --- Proteins, DNA Recombinant --- Proteins, Recombinant --- Recombinant DNA Proteins --- Recombinant Proteins, DNA --- Biotechnology --- Bioreactors --- Dairy Industry --- Dairy Industries --- Industries, Dairy --- Industry, Dairy --- Beef Cow --- Bos grunniens --- Bos indicus --- Bos taurus --- Cow --- Cow, Domestic --- Dairy Cow --- Holstein Cow --- Yak --- Zebu --- Beef Cows --- Cow, Beef --- Cow, Dairy --- Cow, Holstein --- Cows --- Dairy Cows --- Domestic Cow --- Domestic Cows --- Yaks --- Zebus --- Growth Hormone, Pituitary --- Growth Hormones Pituitary, Recombinant --- Pituitary Growth Hormones, Recombinant --- Recombinant Growth Hormones --- Recombinant Pituitary Growth Hormones --- Recombinant Somatotropins --- Somatotropins, Recombinant --- Growth Hormone, Recombinant --- Pituitary Growth Hormone --- Recombinant Growth Hormone --- Somatotropin --- Somatotropin, Recombinant --- Growth Hormones, Recombinant --- Recombinant Somatotropin --- Bovine somatotropin --- Genetically engineered bovine somatotropin --- rBGH (Hormone) --- Recombinant bovine growth hormone --- Recombinant proteins --- Food Adulteration --- Adulteration, Food --- Adulterations, Food --- Contamination, Food --- Contaminations, Food --- Food Adulterations --- Food Contaminations --- Healthcare Policy --- National Health Policy --- Health Policies --- Health Policy, National --- Healthcare Policies --- National Health Policies --- Policy, Health --- Policy, Healthcare --- Policy, National Health --- Government policy --- Economic aspects --- Genetic engineering --- Agronomy --- Agronomies --- Health Care Policies --- Care Policies, Health --- Health Care Policy --- Policies, Health --- Policies, Health Care --- Policies, Healthcare --- Policy, Health Care