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The majority of microbes in many environments are considered “as yet uncultured” and were traditionally considered inaccessible for study through the microbiological gold standard of pure culture. The emergence of metagenomic approaches has allowed researchers to access and study these microbes in a culture-independent manner through DNA sequencing and functional expression of metagenomic DNA in a heterologous host. Metagenomics has revealed an extraordinary degree of diversity and novelty, not only among microbial communities themselves, but also within the genomes of these microbes. This Research Topic aims to showcase the utility of metagenomics to gain insights on the microbial and genomic diversity in different environments by revealing the breadth of novelty that was in the past, largely untapped.
Human microbiome --- Metagenomics --- metatranscriptomics --- DNA SEQUENCING --- functional metagenomics --- viral metagenomics
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The majority of microbes in many environments are considered “as yet uncultured” and were traditionally considered inaccessible for study through the microbiological gold standard of pure culture. The emergence of metagenomic approaches has allowed researchers to access and study these microbes in a culture-independent manner through DNA sequencing and functional expression of metagenomic DNA in a heterologous host. Metagenomics has revealed an extraordinary degree of diversity and novelty, not only among microbial communities themselves, but also within the genomes of these microbes. This Research Topic aims to showcase the utility of metagenomics to gain insights on the microbial and genomic diversity in different environments by revealing the breadth of novelty that was in the past, largely untapped.
Human microbiome --- Metagenomics --- metatranscriptomics --- DNA SEQUENCING --- functional metagenomics --- viral metagenomics
Choose an application
The majority of microbes in many environments are considered “as yet uncultured” and were traditionally considered inaccessible for study through the microbiological gold standard of pure culture. The emergence of metagenomic approaches has allowed researchers to access and study these microbes in a culture-independent manner through DNA sequencing and functional expression of metagenomic DNA in a heterologous host. Metagenomics has revealed an extraordinary degree of diversity and novelty, not only among microbial communities themselves, but also within the genomes of these microbes. This Research Topic aims to showcase the utility of metagenomics to gain insights on the microbial and genomic diversity in different environments by revealing the breadth of novelty that was in the past, largely untapped.
Human microbiome --- Metagenomics --- metatranscriptomics --- DNA SEQUENCING --- functional metagenomics --- viral metagenomics
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Sequence Analysis --- Microarray Analysis --- Informatics. --- instrumentation. --- high-dimensional approaches --- life sciences --- DNA Sequencing --- bioinformatics --- dna sequencing --- Information science
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This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact
reservoirs --- lakes --- surface water --- sediments --- DNA sequencing --- CARD-FISH --- ARGs --- freshwater
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This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact
Science: general issues --- Medical microbiology & virology --- Microbiology (non-medical) --- reservoirs --- lakes --- surface water --- sediments --- DNA sequencing --- CARD-FISH --- ARGs --- freshwater
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This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact
Science: general issues --- Medical microbiology & virology --- Microbiology (non-medical) --- reservoirs --- lakes --- surface water --- sediments --- DNA sequencing --- CARD-FISH --- ARGs --- freshwater
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menselijk genoom --- genetica (genen) --- moleculaire biologie --- genetisch onderzoek --- voorspellende geneeskunde (predictieve geneeskunde) --- génome humain --- génétique (gènes) --- biologie moléculaire --- recherche génétique --- médecine prédictive --- DNA SEQUENCING --- GENE THERAPY --- ETHICS --- METHODS
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DNA polymerases are core tools for molecular biology including PCR, whole genome amplification, DNA sequencing and genotyping. Research has focused on discovery of novel DNA polymerases, characterization of DNA polymerase biochemistry and development of new replication assays. These studies have accelerated DNA polymerase engineering for biotechnology. For example, DNA polymerases have been engineered for increased speed and fidelity in PCR while lowering amplification sequence bias. Inhibitor resistant DNA polymerase variants enable PCR directly from tissue (i.e. blood). Design of DNA polymerases that efficiently incorporate modified nucleotide have been critical for development of next generation DNA sequencing, synthetic biology and other labeling and detection technologies. The Frontiers in Microbiology Research Topic on DNA polymerases in Biotechnology aims to capture current research on DNA polymerases and their use in emerging technologies.
Animal Biochemistry --- Human Anatomy & Physiology --- Health & Biological Sciences --- DNA polymerase evolution --- DNA polymerase fidelity --- molecular diagnostics --- DNA polymerase --- PCR inhibitors --- next generation sequencing --- PCR --- DNA sequencing
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Molecular biology --- Molecular cloning --- Recombinant DNA --- MOLECULAR BIOLOGY --- CLONING --- DNA, RECOMBINANT --- GENES --- GENOMIC LIBRARIES --- NUCLEIC ACIDS --- DNA SEQUENCING --- POLYMERASE CHAIN REACTION --- ESCHERICHIA COLI --- GENE EXPRESSION --- LABORATORY MANUALS --- BIOTECHNOLOGY --- ISOLATION TECHNIQUES --- LABELLING --- VISUALIZING --- HYBRIDIZATION --- RECOMBINANT PROTEINS --- PRODUCTION
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