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This volume details a wide range of methods, ranging from beginner through advanced, used to further study zebrafish and fish germline.. Chapter guide readers through cultivating and manipulating germ cells, imaging of germline processes and the molecular analysis of their, protein, and RNA. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Germline Development in the Zebrafish: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Peix zebra --- Biologia del desenvolupament --- Manuals de laboratori --- Tècniques de laboratori --- Desenvolupament (Biologia) --- Biologia --- Cicles vitals (Biologia) --- Desenvolupament de les plantes --- Desenvolupament infantil --- Envelliment --- Evolució --- Miogènesi --- Formació de models (Biologia) --- Genètica del desenvolupament --- Larves --- Neurobiologia del desenvolupament --- Creixement --- Embriologia --- Ontogènesi --- Danio --- Danio rerio --- Peixos zebra --- Rerio --- Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells
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The molecular biology revolution has transformed developmental biology into one of the most exciting and fruitful fields in experimental biomedical research today. In Developmental Biology Protocols, established leaders in this field demonstrate this achievement with a comprehensive collection of cutting-edge protocols for studying and analyzing the events of embryonic development. Drawing on state-of-the-art cellular and molecular techniques, as well as new and sophisticated imaging and information technologies, this first volume of three presents readily reproducible methods for establishing and characterizing several widely used experimental model systems, for both the study of developmental patterns and morphogenesis, and the examination of embryo structure and function. In addition, there are step-by-step methods for the analysis of cell lineage, the production and use of chimeras, and the experimental molecular manipulation of embryos, including the application of viral vectors. The second and third volumes in this seminal set complete today's widest-ranging collection of techniques designed to decipher the exact cellular, molecular, and genetic mechanisms that control the form, structure, and function of the developing embryo. Volume 2 describes state-of-the-art methods for the study of organogenesis, the analysis of abnormal development and teratology, the screening and mapping of novel genes and mutations, and the application of transgenesis, including the production of transgenic animals and gene knockouts. No less innovative, Volume 3 introduces powerful techniques for the manipulation of developmental gene expression and function, the analysis of gene expression, the characterization of tissue morphogenesis and development, the in vitro study of differentiation and development, and the genetic analysis of developmental models of diseases. Highly practical and richly annotated, the three volumes of Developmental Biology Protocols describe multiple experimental systems and details techniques adopted from the broadest array of biomedical disciplines. Every researcher will not only better understand the principles, background, and rationale for how form and function are elaborated in an organism, but also gain full practical access to today's best methods for its analysis.
Developmental biology. --- Developmental Biology. --- Development (Biology) --- Biology --- Growth --- Ontogeny --- Biologia del desenvolupament --- Protocols clínics --- Plans d'actuació mèdica --- Protocols en medicina --- Protocols de diagnòstic clínic --- Protocols mèdics --- Protocols terapèutics --- Medicina clínica --- Històries clíniques --- Protocols d'infermeria --- Desenvolupament (Biologia) --- Biologia --- Cicles vitals (Biologia) --- Desenvolupament de les plantes --- Desenvolupament infantil --- Envelliment --- Evolució --- Miogènesi --- Formació de models (Biologia) --- Genètica del desenvolupament --- Larves --- Neurobiologia del desenvolupament --- Creixement --- Embriologia --- Ontogènesi
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This detailed new edition aims to provide a range of methods and protocols for studying vascular morphogenesis in vivo and in vitro to reflect up-to-date advances in the field. Of note, the book explores endothelial cell signaling is currently understood to promote fundamental cues for cell fate specification, embryo patterning, organ differentiation, and postnatal tissue remodeling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and timely, Vascular Morphogenesis: Methods and Protocols, Second Edition will serve as an ideal guide to this area of study for a broad audience amongst cell biologists, anatomists, pathologists, and physiologists.
Morfogènesi --- Biologia del desenvolupament --- Desenvolupament (Biologia) --- Biologia --- Cicles vitals (Biologia) --- Desenvolupament de les plantes --- Desenvolupament infantil --- Envelliment --- Evolució --- Miogènesi --- Formació de models (Biologia) --- Genètica del desenvolupament --- Larves --- Neurobiologia del desenvolupament --- Creixement --- Embriologia --- Ontogènesi --- Morfogènia --- Diferenciació cel·lular --- Organogènesi --- Morfologia (Biologia) --- Developmental biology. --- Cytology. --- Developmental Biology and Stem Cells. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Development (Biology) --- Growth --- Ontogeny --- Biologia del desenvolupament. --- Citologia --- Biologia cel·lular --- Citogenètica --- Citoquímica --- Migració cel·lular --- Cèl·lules --- Cèl·lules animals --- Ultraestructura (Biologia)
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This volume details commonly used molecular and cellular techniques and specialized methodologies for studying retina neuronal subtypes and electrophysiology. Chapters describe techniques for anatomical studies of retinal ganglion cell morphology, gap-junction-mediated neuronal connection, multi-electrode array recording on mouse retinas, and paired recording to study the electrical coupling between photoreceptors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Retinal Development: Methods and Protocols aims to provide readers with a set of practical experimental tools to study retinal development, regeneration, and function of mature retinal neurons. Many of the protocols and strategies described in one organism can be easily adapted to applications in different model systems.
Retina --- Posterior segment (Eye) --- Electric properties. --- Cytology. --- Citologia --- Biologia cel·lular --- Biologia --- Citogenètica --- Citoquímica --- Migració cel·lular --- Cèl·lules --- Cèl·lules animals --- Ultraestructura (Biologia) --- Ull --- Developmental biology. --- Neurosciences. --- Developmental Biology and Stem Cells. --- Neuroscience. --- Neural sciences --- Neurological sciences --- Neuroscience --- Medical sciences --- Nervous system --- Development (Biology) --- Biology --- Growth --- Ontogeny --- Neurociències --- Biologia del desenvolupament --- Desenvolupament (Biologia) --- Cicles vitals (Biologia) --- Desenvolupament de les plantes --- Desenvolupament infantil --- Envelliment --- Evolució --- Miogènesi --- Formació de models (Biologia) --- Genètica del desenvolupament --- Larves --- Neurobiologia del desenvolupament --- Creixement --- Embriologia --- Ontogènesi --- Neurociència --- Ciències neurològiques --- Ciències de la salut --- Neuroanatomia --- Neurobiologia --- Neurociència computacional --- Neuroendocrinologia --- Neurofarmacologia --- Neurogenètica --- Neuroquímica --- Sistema nerviós
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This reference work provides an comprehensive and easily accessible source of information on numerous aspects of Evolutionary Developmental Biology. The work provides an extended overview on the current state of the art of this interdisciplinary and dynamic scientific field. The work is organized in thematic sections, referring to the specific requirements and interests in each section in far detail. “Evolutionary Developmental Biology – A Reference Guide” is intended to provide a resource of knowledge for researchers engaged in evolutionary biology, developmental biology, theoretical biology, philosophy of sciences and history of biology.
Evolutionary developmental biology. --- Developmental evolution (Biology) --- Evo-devo (Evolutionary developmental biology) --- Evolution of development (Biology) --- Evolutionary biology of development --- Developmental biology --- Evolution (Biology) --- Biologia del desenvolupament --- Evolució (Biologia) --- Biologia evolutiva --- Darwinisme --- Evolució animal --- Evolució biològica --- Evolució dels animals --- Evolució dels éssers vius --- Origen de les espècies --- Biologia --- Evolució --- Evolució de la conducta --- Evolució del cervell --- Energia exosomàtica --- Epigènesi --- Evolució humana --- Origen de l'home --- Paleobiologia evolutiva --- Extinció (Biologia) --- Filogènia --- Selecció natural --- Desenvolupament (Biologia) --- Cicles vitals (Biologia) --- Desenvolupament de les plantes --- Desenvolupament infantil --- Envelliment --- Miogènesi --- Formació de models (Biologia) --- Genètica del desenvolupament --- Larves --- Neurobiologia del desenvolupament --- Creixement --- Embriologia --- Ontogènesi --- Evolution (Biology). --- Developmental biology. --- Biology --- Evolutionary Biology. --- Developmental Biology and Stem Cells. --- Philosophy of Biology. --- Philosophy. --- Vitalism --- Development (Biology) --- Growth --- Ontogeny --- Animal evolution --- Animals --- Biological evolution --- Darwinism --- Evolutionary biology --- Evolutionary science --- Origin of species --- Evolution --- Biological fitness --- Homoplasy --- Natural selection --- Phylogeny
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