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With the proliferation of fluorescence in situ hybridization (FISH) techniques as a powerful diagnostic tool in many areas of medicine, the variety and complexity of tests pose bewildering choices even for long-experienced laboratory technologists and physicians. In Molecular Cytogenetics: Protocols and Applications, Yao-Shan Fan and a panel of senior scientists and pioneering researchers describe in step-by-step fashion not only the leading FISH techniques, but also those molecular technologies beyond FISH now available for diagnostic services in genetics and oncology. The methods include labeling FISH probes for DNA and RNA targets, fluorescence genotyping, CGH microarray, spectral karyotyping/multicolor FISH, and primed in situ labeling. There are also techniques for multicolor fiber FISH, multi-telomere FISH, prenatal diagnosis using maternal blood, and preimplantation diagnosis. Oncological methods include simultaneous fluorescence immunophenotyping and FISH for leukemia and lymphoma, HER2 amplification in breast cancer, and CAC/PAC for cancer cytogenetics. Each easy-to-follow protocol offers first hand advice on how to obtain the best results, to avoid pitfalls, and to troubleshoot problems. Many protocols discuss possible future applications of the technology and explain its sensitivity and limitations. Cutting-edge and highly practical, Molecular Cytogenetics: Protocols and Applications offers laboratory physicians and scientists today's most comprehensive and up-to-date collection of FISH protocols for cancer diagnosis and investigation, plus an array of the emerging molecular technologies beyond FISH.

Fluorescence in situ hybridization --- FISH (Fluorescence in situ hybridization) --- Fluorescent in situ hybridization --- Cytogenetics --- Molecular genetics --- Fluorescence microscopy --- In situ hybridization --- Genetics --- Molecular biology --- Cytology --- Human genetics. --- Human Genetics. --- Heredity, Human --- Human biology --- Physical anthropology

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Classical histology has been augmented by immunohistochemistry (the use of specific antibodies to stain particular molecular species in situ). Immunohistochemistry has allowed the identification of many more cell types than could be visualized by classical histology, particularly in the immune system and among the scattered hormone-secreting cells of the endocrine system. This book discusses all aspects of immunohistochemistry and in situ hybridization technologies and the important role they play in reaching a cancer diagnosis. It provides step-by-step instructions on the methods

Cancer --- Immunohistochemistry. --- In situ hybridization. --- Fluorescence in situ hybridization. --- Immunodiagnosis. --- FISH (Fluorescence in situ hybridization) --- Fluorescent in situ hybridization --- Fluorescence microscopy --- In situ hybridization --- Nucleic acid hybridization --- Immunohistology --- Histochemistry --- Immunochemistry --- Diagnosis --- Immunological aspects

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Molecular and cell morphological methods have increased rapidly in number and versatility in recent years and, with the completion of the human genome project, will come into much greater demand for the study of human genes and diseases. In Morphology Methods: Cell and Molecular Biology Techniques, Ricardo Lloyd, md, phd, provides critical and tutorial surveys of the cutting-edge molecular and cell biologic techniques used today in the analysis of cells and tissues in situ. These molecular-based histologic and morphologic techniques are described in step-by-step detail by hands-on experts, often those who have originated or perfected them. Among the techniques presented are laser capture microdissection, in situ hybridization, FISH, confocal laser scanning microscopy, in situ PCR, and tyramide amplification. Applications include the detection of infectious agents and DNA, the diagnosis of undifferentiated tumors and endocrine lesions, the metaphase analysis of whole blood cultures, and clonality analysis. Valuable notes on potential pitfalls and practical tips designed to ensure success are included with each method. In its integrated, state-of-the-art approach, Morphology Methods: Cell and Molecular Biology Techniques thoroughly details and applies the rich panoply of significant new cell and molecular biologic technologies to all the major problems confronting morphologic studies and diagnostic pathology today.

Cytology --- Fluorescence in situ hybridization. --- Immunohistochemistry. --- Molecular biology --- Polymerase chain reaction. --- Technique. --- Biological techniques --- Human histology. Human cytology --- Pathology. --- Disease (Pathology) --- Medical sciences --- Diseases --- Medicine --- Medicine, Preventive --- Chain reaction, Polymerase --- PCR (Biochemistry) --- Polymerization --- DNA polymerases --- Immunohistology --- Histochemistry --- Immunochemistry --- FISH (Fluorescence in situ hybridization) --- Fluorescent in situ hybridization --- Fluorescence microscopy --- In situ hybridization