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This second edition provides updated and comprehensive methods on miRNA biogenesis and their role in the development and progression of various human diseases. Chapters detail miRNA biogenesis, isolating RNA, extracellular vesicles (EVs), circulating miRNAs, analyzing miRNA and miRDeep-P2, protocols for total RNA isolation from cells, cell-derived products, isolation and characterization of exosomes, serum, plasma specimens, and software tools. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, MicroRNA Profiling: Methods and Protocols, Second Edition aims to provide comprehensive and accessible methods to undergraduate, graduate, and established scientist.

Genetics. --- Genetics and Genomics.

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This volume provides readers with a comprehensive review of various gene silencing methodologies and its applications. It provides a historical overview of gene silencing mechanisms in plants, vectors, and strategies available for plant gene silencing, practical applications of gene silencing, bioinformatics tools, and other resources. In addition to these review chapters, this book includes methodology for virus-induced gene silencing (VIGS) in various different plant species, understanding plant stress responses using VIGS, miRNA identification, DNA interference, host-induced gene silencing, use of artificial miRNAs for gene silencing, high throughput RNAi, and more. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.  Thorough and practical, Plant Gene Silencing: Methods and Protocols seeks to aid scientists to further understand functional relevance of target genes using gene silencing methods and use these technologies in commercial plant varieties.

Plant genetics. --- Plant Genetics and Genomics. --- Plants --- Genetics

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This open access book covers a century of research on wheat genetics and evolution, starting with the discovery in 1918 of the accurate number of chromosomes in wheat. We re-evaluate classical studies that are pillars of the current knowledge in light of recent genomic data in the wheat group comprising 31 species from the genera Amblyopyrum, Aegilops, Triticum, and other more distant relatives. For these species, we describe morphology, ecogeographical distribution, phylogeny as well as cytogenetic and genomic features. For crops, we also address evolution under human selection, namely pre-domestication cultivation and domestication. We re-examine the genetic and archeological evidence of where, when, and how domestication occurred. Several species are polyploids, including bread wheat which is a young allohexaploid. We discuss unique aspects of genome evolution and maintenance under polyploidization. Finally, we propose some thoughts on the future prospects of wheat improvement. As such, it can be of great interest to wheat researchers and breeders as well as to plant scientists and students interested in plant genetics, evolution, domestication, and polyploidy.

Botany. --- Agriculture. --- Genetics. --- Plant Science. --- Genetics and Genomics.

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This comprehensive, up-to-date reference work compiles the scientific research on the relationship between various forms of cancer and reactive oxygen species (ROS). It is well known that ROS play a key role in the various steps leading to malignancy, this includes increase in DNA mutation rates. The book brings together exciting reviews, written by leading experts all over the world. The book is divided into four broad sections. Each section describes the role of oxidative stress and underlying mechanisms and pathways, at various stages of cancer, including its role in carcinogenesis, cancer progression and metastasis. Further, the book describes how the reactive oxygen species (ROS) modulate gene expression and signal transduction pathways in cancer. One section of the book is also dedicated to the therapeutics in ROS-induced cancer. It discusses the ways to interfere with the cancer progression, and design anti-cancer drugs. The book provides an integrated approach for better understanding of the development phases of oxidative stress-induced cancers, and the current perspectives about therapeutics of cancers. It is a highly resourceful book and would be extremely important to clinicians, students and researchers in the field of cancer genetics, therapeutics and oncology. .

Cancer research. --- Genetics. --- Cancer Research. --- Genetics and Genomics.

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Lieber EXPERIMENTATOR, wir präsentieren Ihnen hier das Grundlagenwissen sowie Tipps und Tricks für den Umgang mit Nucleinsäuren. Sie werden sofort merken, dass der Autor Lust und Frust der täglichen Laborroutine genau kennt. Aus dem Inhalt: Präparieren, Fällen, Konzentrieren und Reinigen von Nucleinsäuren Das molekularbiologische Handwerkszeug (Restriktionsenzyme, Gele, Blotten) PCR (Polymerase-Kettenreaktion) RNA-Isolierung, -Transkription Klonierung von DNA-Fragmenten DNA-Nachweis und -Analyse (Markierung von Sonden, Hybridisierung, Screening, Sequenzierung) Modifikation und Funktionsanalyse von DNA-Sequenzen (Mutagenese, In-vitro-Translation, transgene Mäuse, Transgenexpression, Gentherapie, Genomik) Lieferantenverzeichnis Protokoll-Sammlungen gibt es viele, aber wer erklärt einem, was sich hinter den Methoden verbirgt? Dieses Buch richtet sich an alle Experimentatoren, die molekularbiologische Versuche durchführen wollen und gern nachvollziehen möchten, was sich in ihrem Reaktionsgefäß abspielt. Das ganze Spektrum der üblichen molekularbiologischen Methoden wird vorgestellt, kommentiert und Alternativen aufgezeigt. Und weil die Laborversuche nicht alles sind, findet sich auch noch ein Kapitel zum Thema Karriereplanung. Der lockere Ton wendet sich gleichermaßen an Studenten wie an BTAs und Laboranten, aber auch der alte Hase wird hier und dort noch etwas Neues entdecken. Die 7. Auflage wurde überarbeitet und aktualisiert. Über den Autor: Dr. Cornel Mülhardt. Geboren 1965. Biologie-Studium in Heidelberg und Hamburg. Wissenschaftlicher Angestellter in Erlangen (Institut für Med. Biochemie). Seit 1998 tätig bei Hoffmann-La Roche (Basel) in der Forschungsrichtung Neurobiologie. Seit Mai 2001 Leiter der Biologielaboranten-Ausbildung. Rezensionen der Vorauflagen: Wer sich einen realistischen Überblick über den Stand der Technik in der Molekularbiologie verschaffen will oder gar selber im Labor molekularbiologisch tätig werden (sein) möchte, muss dieses Buch lesen, chemikalienresistent einschlagen und ständig bei sich tragen! BioTec Zeigt Auswege ausexperimentellen Sackgassen und weckt ein Gespür für das richtige Experiment zur rechten Zeit. Bioworld.

Cell biology. --- Animal genetics. --- Microbial genetics. --- Microbial genomics. --- Proteomics. --- Cell Biology. --- Animal Genetics and Genomics. --- Microbial Genetics and Genomics. --- Proteomics.