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As the use of high-throughput screening expands and creates more interest in the academic community, the need for detailed reference materials becomes ever more pressing. Cell-Based Assays for High-Throughput Screening: Methods and Protocols aims to fill an important part of this need by providing an easily accessible reference volume for cell-based phenotypic screening. Leading researchers in the field contribute state-of-the-art methods with actionable protocols covering four major areas of study: model biological systems, screening modalities and assay systems, detection technologies, and approaches to data analysis. Written in the highly successful Methods in Molecular Biology™ series format, each chapter includes a brief introduction to the subject, lists of necessary materials and reagents, step-by-step laboratory protocols, and a Notes section detailing tips on troubleshooting and avoiding known pitfalls. Cutting-edge and easy-to-use, Cell-Based Assays for High-Throughput Screening: Methods and Protocols presents an overview of relevant approaches, enabling the direct application of existing methods to new discoveries while also inspiring researchers to approach their screening projects in a conceptually modular fashion, enhancing the power to discover through new combinations of existing approaches.

Biochemistry. --- Gene libraries. --- Biochemistry, general. --- Combinatorial Libraries. --- Gene banks (Genetic engineering) --- Genomic banks --- Genomic libraries --- Biological resource centers --- Genomes --- Recombinant DNA --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition

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The establishment, analysis, prediction, and expansion of a comprehensive ligand-target Structure-Activity Relationship (SAR) in the post-genomic era presents a key research challenge for this century. In Chemogenomics: Methods and Applications, experts from academia and industry explore cutting-edge in vitro and in silico approaches available today and outline the relevant aspects of chemistry, biology, and molecular informatics which are the cornerstones of chemogenomics, thus combining introductory concepts with detailed methods and protocols. Covering topics such as target family-oriented compound library design, drug discovery targeting the purinome and co-factor binding sites, as well as the pocketome engine and molecular interaction field approaches, the book emphasizes systemization as an advantage to furthering the difficult science of drug discovery. As a volume in the highly successful Methods in Molecular Biology™ series format, this work provides the kind of detailed description and implementation advice that is crucial for getting optimal results. Authoritative and practical, Chemogenomics: Methods and Applications presents content which will be of enormous value to those striving for the discovery of new and better therapies for diseases.

Biochemistry. --- Human genetics. --- Analytical biochemistry. --- Gene libraries. --- Biochemistry, general. --- Human Genetics. --- Analytical Chemistry. --- Combinatorial Libraries. --- Gene banks (Genetic engineering) --- Genomic banks --- Genomic libraries --- Biological resource centers --- Genomes --- Recombinant DNA --- Analytic biochemistry --- Biochemistry --- Chemistry, Analytic --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Genetics --- Heredity, Human --- Human biology --- Physical anthropology --- Composition --- Bioanalytic chemistry --- Bioanalytical chemistry --- Analytical chemistry

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The first libraries of complementary DNA (cDNA) clones were con­ structed in the mid-to-late 1970s using RNA-dependent DNA polymerase (reverse transcriptase) to convert poly A* mRNA into double-stranded cDNA suitable for insertion into prokaryotic vectors. Since then cDNA technology has become a fundamental tool for the molecular biologist and at the same time some very significant advances have occurred in the methods for con­ structing and screening cDNA libraries. It is not the aim of cDNA Library Protocols to give a comprehensive review of all cDNA library-based methodologies; instead we present a series of up-to-date protocols that together should give a good grounding of proce­ dures associated with the construction and use of cDNA libraries. In deciding what to include, we endeavored to combine up-to-date versions of some of the most widely used protocols with some very usefiil newer techniques. cDNA Library Protocols should therefore be especially useful to the investigator who is new to the use of cDNA libraries, but should also be of value to the more experienced worker. Chapters 1—5 concentrate on cDNA library construction and manipula­ tion, Chapters 6 and 7 describe means of cloning difficult-to-obtain ends of cDNAs, Chapters 8-18 give various approaches to the screening of cDNA libraries, and the remaining chapters present methods of analysis of cDNA clones including details of how to analyze cDNA sequence data and how to make use of the wealth of cDNA data emerging from the human genome project.

DNA, Complementary --- Gene Library --- Genetic Techniques --- Antisense DNA --- DNA --- Gene libraries --- Molecular cloning --- ADN --- Clonage moléculaire --- Laboratory manuals --- Manuels de laboratoire --- 57.088 --- 577.212.3 --- -DNA --- -Gene libraries --- -Molecular cloning --- -#ABIB:aimm --- Cloning, Molecular --- DNA cloning --- Gene cloning --- Cloning --- Genetic engineering --- Molecular genetics --- Clone cells --- Gene banks (Genetic engineering) --- Genomic banks --- Genomic libraries --- Biological resource centers --- Genomes --- Recombinant DNA --- Deoxyribonucleic acid --- Desoxyribonucleic acid --- Thymonucleic acid --- TNA (Nucleic acid) --- Deoxyribose --- Nucleic acids --- Genes --- Complementary DNA --- Antisense nucleic acids --- Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods --- Nucleic acid base and sequence compositon. Experimental deciphering of genetic code. --- Antisense DNA -- Laboratory manuals. --- DNA -- Laboratory manuals. --- Electronic books. -- local. --- Gene libraries -- Laboratory manuals. --- Molecular cloning -- Laboratory manuals. --- DNA, Single-Stranded --- DNA Probes --- Genetic Structures --- Investigative Techniques --- Nucleic Acid Probes --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Genetic Phenomena --- Nucleic Acids --- Phenomena and Processes --- Molecular Probes --- Laboratory Chemicals --- Nucleic Acids, Nucleotides, and Nucleosides --- Chemicals and Drugs --- Specialty Uses of Chemicals --- Chemical Actions and Uses --- Genetics --- Biology --- Health & Biological Sciences --- DNA, Complementary. --- Gene Library. --- Genetic Techniques. --- Laboratory manuals. --- 577.212.3 Nucleic acid base and sequence compositon. Experimental deciphering of genetic code. --- 57.088 Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods --- Antisense dna --- Dna --- Dna, complementary. --- Gene library. --- Genetic techniques. --- Clonage moléculaire --- #ABIB:aimm --- Nucleic acid base and sequence compositon. Experimental deciphering of genetic code --- Cytology. --- Immunology. --- Cell Biology. --- Immunobiology --- Life sciences --- Serology --- Cell biology --- Cellular biology --- Cells --- Cytologists