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This book compiles key protocols instrumental to the study of high-throughput protein production and purification which have been refined and simplified over the years and are now ready to be transferred to any laboratory. Beginning with a section covering general procedures for high-throughput protein production, the volume continues with high-throughput protocols adapted to the production of specific protein families, as well as an extensive section on protocols combining high-throughput protein production and their micro-characterization. Written for the highly successful Methods in Molecular Biology series, chapters in this book include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, High-Throughput Protein Production and Purification: Methods and Protocols serves biochemists ranging from engineers, PhD students and post-doctoral fellows, to the heads of protein expression facilities and researchers, in pursuing this vital area of study.
Proteins --- Protein Biosynthesis. --- Combinatorial Chemistry Techniques. --- High-Throughput Screening Assays. --- Protéines --- Synthèse protéique. --- isolation & purification. --- Biotechnologie. --- Purification.
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Vaccines --- Vaccination --- Vaccins --- Periodicals. --- Periodicals --- Périodiques --- Drug Discovery. --- Therapeutic use --- therapeutic use. --- Drug Prospecting --- Discovery, Drug --- Prospecting, Drug --- Biologicals --- High-Throughput Screening Assays --- Bioprospecting --- Drug Discovery --- therapeutic use
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The medicinal use of plants, animals and microorganisms has been a part of human evolution and likely began before recorded history. Is it possible that this knowledge can be used to create powerful new drugs and solve some of the human health problems facing us today? This book is a collection of an expert team of agronomists, chemists, biologists and policy makers who discuss some of the processes involved in developing a naturally-sourced bioactive compound into a drug therapy. These experts define a natural compound and elucidate the processes required to find, extract and define a naturally-derived bioactive molecule. Finally, they describe the necessity for understanding the fundamental mechanisms of disease before applying bioactive molecules in bioassay-guided drug discovery platforms.
Drug Discovery. --- Drug Prospecting --- Discovery, Drug --- Prospecting, Drug --- High-Throughput Screening Assays --- Bioprospecting --- Health Care --- Toxicology and Pharmaceutical Science --- Pharmacology --- Drug Discovery --- Health Sciences
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Drug development. --- Development of drugs --- Drugs --- New drug development --- Pharmacology --- Pharmacy --- Development --- Imidazoles. --- Drug Discovery. --- Drug Prospecting --- Discovery, Drug --- Prospecting, Drug --- High-Throughput Screening Assays --- Bioprospecting --- Benzimidazoles
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This book, Natural Products and Cancer Drug Discovery, is written by leading experts in natural products in cancer therapy. The first two sections describe new applications of common herbs and foods for treatment of cancer. Section 3 deals with the development of new chemotherapeutics from Cannabis and endophytic fungi. Section 4 presented formulations of natural products for treatment of malignant melanoma. Made-to-order anticancer therapy from natural products using computational and tissue engineering approaches is addressed in the fifth section. It is our hope that this book may motivate readers to approach the evidence of anticancer natural products with an open mind and thereby spark an interest in making further contributions to the cancer treatment efforts.
Natural products. --- Drug Discovery. --- Cancer. --- Cancers --- Carcinoma --- Malignancy (Cancer) --- Malignant tumors --- Tumors --- Products, Natural --- Raw materials --- Drug Prospecting --- Discovery, Drug --- Prospecting, Drug --- High-Throughput Screening Assays --- Bioprospecting --- Medicine --- Chronooncology --- Oncology --- Health Sciences
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Vaccines --- Immunotherapy. --- Drug Discovery. --- Vaccines. --- Periodicals. --- therapeutic use. --- Biologicals --- Drug Prospecting --- Discovery, Drug --- Prospecting, Drug --- High-Throughput Screening Assays --- Bioprospecting --- Immunotherapies --- Immunity, Active --- Immunologic Techniques --- Periodical. --- Vaccine --- Combined Antibody Therapeutics
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Privileged Scaffolds in Drug Discovery is the most complete and up-to-date work in the area. Covering a wide range of privileged structures, it is a perfect reference for scientists involved in targeted drug development. The editors recruited experts from several prestigious Chinese institutions to cover the areas of antiviral drugs, chalcone, pyrimidine, (benz)imidazoles, natural product-derived privileged scaffolds, N-Sulfonyl carboxamides, kinase inhibitors, antitumor molecules, antineurodegenerative drugs, triazoles, oxazolidinone, indole and indoline scaffolds, tigliane diterpenoids, peptide and peptide-based drugs, quassinoids, and others including pseudonatural products, macrocycles, stable peptides and peptidomimetics. The book also explores scaffolds in drug molecules approved in recent years. Privileged Scaffolds in Drug Discovery is a complete reference for researchers in drug discovery and organic synthesis, in academic and corporate settings, who are investigating privileged structures upon which to base new drugs. Researchers in medicinal chemistry and chemical biology will also find the contents of this book valuable.
Drug Discovery. --- Chemistry, Pharmaceutical. --- Chemistry, Pharmaceutic --- Pharmaceutic Chemistry --- Pharmaceutical Chemistry --- Medicinal Chemistry --- Chemistry, Medicinal --- Drug Prospecting --- Discovery, Drug --- Prospecting, Drug --- High-Throughput Screening Assays --- Bioprospecting --- Drug development. --- Pharmaceutical chemistry --- Drug Discovery --- Chemistry, Pharmaceutical
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Drug Discovery. --- Drug Industry --- organization & administration. --- Farmacologie (geneesmiddelenleer) --- 615.012 --- Production of medicaments (drugs, medicines, remedies). Preparation and purification --- 615.012 Production of medicaments (drugs, medicines, remedies). Preparation and purification --- Drug Discovery --- Drug Prospecting --- Discovery, Drug --- Prospecting, Drug --- High-Throughput Screening Assays --- Bioprospecting --- organization & administration
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Drug Discovery. --- Drug Therapy. --- Technology, Pharmaceutical. --- Pharmaceutic Technology --- Pharmaceutical Technology --- Pharmacy Technology --- Technology, Pharmaceutic --- Technology, Pharmacy --- Therapy, Drug --- Chemotherapy --- Pharmacotherapy --- Chemotherapies --- Drug Therapies --- Pharmacotherapies --- Therapies, Drug --- Disease --- Pharmaceutical Preparations --- Drug Prospecting --- Discovery, Drug --- Prospecting, Drug --- drug therapy --- therapeutic use --- Chemistry, Pharmaceutical --- Drug Industry --- Pharmacologic Actions --- High-Throughput Screening Assays --- Bioprospecting
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Pharmacology --- Pharmacology. --- Drug effects --- Medical pharmacology --- Medical sciences --- Chemicals --- Chemotherapy --- Drugs --- Pharmacy --- Pharmacologies --- Pharmaceutical Preparations --- Physiological effect --- pharmacology --- drug discovery --- pharmacotherapy --- drug action --- pharmacogenetics --- Drug Discovery --- Drug Prospecting --- Discovery, Drug --- Prospecting, Drug --- High-Throughput Screening Assays --- Bioprospecting --- Pharmaceutic Preparations --- Pharmaceutical Products --- Pharmaceuticals --- Preparations, Pharmaceutical --- Drug --- Pharmaceutical --- Pharmaceutical Preparation --- Pharmaceutical Product --- Preparation, Pharmaceutical --- Preparations, Pharmaceutic --- Product, Pharmaceutical --- Products, Pharmaceutical --- Drug Dosage Calculations
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