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mRNA processing is a key step in gene expression that effects all the proteins within the cell. In mRNA Processing and Metabolism: Methods and Protocols, world-renowned researchers bring together the latest techniques spanning the breadth of mRNA processing and metabolism. Drawing on recent advances in microscopy, whole genome sequencing, microarrays, mass spectrometry, fluorescent detection methodologies, and RNA interference, the authors offer readily reproducible methods for the cotranscriptional processing events that occur while the mRNA is engaged with elongating RNA polymerase II, with splicing and its biochemical analysis and with alternative splicing. Additional methods cover mRNA export, the recovery and analysis of mRNP complexes, cytoplasmic translation, mRNA degradation in vivo and in vitro, and the controversial concept of nuclear translation. A variety of model organisms are used, including yeast, Drosophila, Xenopus, mice, plants, and cultured mammalian cells. Each proven protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Timely and authoritative, mRNA Processing and Metabolism: Methods and Protocols provides a powerful collection of novel techniques that are not only effective, but also immediately applicable to current problems in many areas of biological research.

Messenger RNA --- ARN messager --- Laboratory manuals --- Manuels de laboratoire --- Electronic books. -- local. --- Genetics -- Technique. --- Messenger RNA -- Laboratory manuals. --- RNA, Messenger --- Genetic Techniques --- Metabolism --- Metabolic Phenomena --- RNA --- Investigative Techniques --- Nucleic Acids --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Phenomena and Processes --- Nucleic Acids, Nucleotides, and Nucleosides --- Chemicals and Drugs --- Animal Biochemistry --- Human Anatomy & Physiology --- Health & Biological Sciences --- 577.216 --- 57.088.6 --- Transfer of inheritance information. Transport of messenger RNA in the cell --- Methods and techniques for studying metabolism and biotransformation.Radioisotopes methods and techniques. --- 57.088.6 Methods and techniques for studying metabolism and biotransformation.Radioisotopes methods and techniques. --- 577.216 Transfer of inheritance information. Transport of messenger RNA in the cell --- Genetics --- Technique. --- Informational RNA --- Messenger ribonucleic acid --- mRNA --- Protein transcript --- Protein transcripts --- Template RNA --- Methods and techniques for studying metabolism and biotransformation.Radioisotopes methods and techniques --- Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists

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Drs. Peng Liang and Arthur B. Pardee have in Differential Display Methods and Protocols assembled for the first time a comprehensive review of the state of the art of their powerful new methodology and its practical applications. The book's pioneering contributors describe all the major elements of this novel technology, including both RAP-PCR and DD using fluorescence detection, as well as their powerful strategy for identifying and cloning family-specific genes. Also provided are numerous examples-along with detailed experimental procedures-in which differentially expressed genes have been successfully identified in diverse biological systems ranging from plants to songbirds to humans. Comprehensive and on the cutting-edge, Differential Display Methods and Protocols provides readers with precise new tools for studying how gene expression is exactly regulated throughout the development of a living organism, and how the failure of this intricate control mechanism leads to pathological complications. This novel and productive methodology, fully detailed here, is already playing a major role in the development of selective antagonists and inhibitors for treating cancer, cardiovascular disease, CNS disorders, and inflammation and tissue repair. Now scientists everywhere can successfully apply this powerful set of techniques using the step-by-step procedures of its innovators.

DNA fingerprinting. --- Gene expression --- Messenger RNA --- Polymerase chain reaction. --- Research --- Methodology. --- Laboratory manuals. --- 57.088.5 --- 577.212.3 --- 577.212.3 Nucleic acid base and sequence compositon. Experimental deciphering of genetic code. --- Nucleic acid base and sequence compositon. Experimental deciphering of genetic code. --- Methods and techniques for studying structures and properties of biological molecules --- Life sciences. --- Cell biology. --- Life Sciences. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Biosciences --- Sciences, Life --- Science --- Polymerase chain reaction --- DNA fingerprinting --- Cloning, Molecular --- Gene Expression --- Molecular Biology --- Polymerase Chain Reaction --- Gene Expression Regulation. --- Methodology --- methods --- physiology --- methods. --- Cytology. --- Messenger RNA - Laboratory manuals --- Gene expression - Research - Methodology --- Cloning, Molecular - methods - laboratory manuals --- Gene Expression - physiology - laboratory manuals --- Molecular Biology - methods - laboratory manuals --- Polymerase Chain Reaction - methods - Laboratory Manuals --- Molecular Biology - methods. --- Cloning, Molecular - methods. --- Polymerase Chain Reaction - methods.