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This book details methods on various aspects of the very final stages of mouse oocyte development. Chapters guide the reader through in vitro growth of follicles, production of a fully-grown competent mouse oocyte, meiosis resumption, analysis of spindle assembly and chromosome segregation, analysis of the oocyte and early embryo transcriptome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mouse Oocyte Development: Methods and Protocols aims to help scientists with the set up both live and fix experiments required to study the process of oocyte meiotic maturation.

Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Òvuls --- Ratolins --- Múrids --- Ratolins transgènics --- Gàmetes femenins --- Òvul --- Oòcits --- Ovòcits --- Embriologia --- Ovaris --- Oogènesi --- Ovulació

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This book provides an up-to-date overview of the various reproductive systems of a variety of aquatic animals, from invertebrates to fishes. While all terrestrial animals use internal fertilization, aquatic animals have diverse reproductive systems. Some are internal fertilizers with or without mating, but many perform external fertilization. Because of this diversity, the reproductive systems of aquatic animals represent excellent models for the study of adaptive evolution and the species specificity of fertilization. In addition, many aquatic animals, including fish, crustaceans, and mollusks, are important as fishery and aquaculture resources. In this book, up-and-coming researchers examine reproductive systems in representative aquatic animals, covering both the basic knowledge and late-breaking results. Reproduction in Aquatic Animals: From Basic Biology to Aquaculture Technology will be of interest to graduate and postgraduate students in biology and agricultural sciences, as well as to researchers and technicians in the fields of reproductive biology and fishery science and to non-academics.

Developmental biology. --- Zoology. --- Cell biology. --- Developmental Biology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Natural history --- Animals --- Development (Biology) --- Growth --- Ontogeny --- Aquatic animals --- Aquaculture. --- Reproduction. --- Aquiculture --- Agriculture --- Aquatic fauna --- Water animals --- Aquatic organisms --- Fauna marina --- Reproducció --- Reproducció animal --- Reproducció sexual --- Biologia --- Vida (Biologia) --- Cèl·lules --- Embaràs --- Fecunditat --- Fetus --- Generació espontània --- Ovulació --- Part --- Partenogènesi --- Reproducció asexual --- Reproducció assistida --- Reproducció de les plantes --- Reproducció humana --- Aparell genital --- Embriologia --- Sexualitat --- Animals marins --- Zoologia marina --- Fauna aquàtica --- Biologia marina --- Invertebrats marins --- Mamífers marins --- Ocells marins --- Peixos marins

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Understanding the reproductive physiology and endocrinology of fishes is essential for captive maturation and seed production in the field of aquaculture. Studying the spermatology of fishes is a comparatively new focus in aquaculture, which has emerged as an important area of fish research over the past two decades. In this regard, the cryopreservation of fish gametes is a crucial aspect. Moreover, energetics studies of gametes have become essential, considering the loss of vigour in the spermatozoa after cryopreservation. The latest development in this context is the cryopreservation of spermatogonial stem cell, which is also covered in the book, along with detailed information on embryo cryopreservation in fishes and crustaceans. The role of cryopreservation in conservation programmes is another important aspect, one that will especially interest biologists. This book addresses central issues in fish gamete cryopreservation and breeding, while also reviewing the history of cryopreservation. Its most unique feature is the breadth of its coverage, from basic information on reproduction in fishes, to such advanced topics as embryo cryopreservation. Chiefly intended as a handy troubleshooting guide, the book represents a valuable resource for research students in related fields.

Cryopreservation of organs, tissues, etc. --- Cryoconservation of organs, tissues, etc. --- Cryogenic preservation of organs, tissues, etc. --- Low temperature preservation of organs, tissues, etc. --- Preservation of organs, tissues, etc. --- Cryobiology --- Cryonics --- Wildlife. --- Fish. --- Animal physiology. --- Biochemistry. --- Conservation biology. --- Ecology . --- Embryology. --- Fish & Wildlife Biology & Management. --- Animal Physiology. --- Animal Biochemistry. --- Conservation Biology/Ecology. --- Animal embryology --- Animals --- Development, Embryological --- Development, Embryonic --- Development, Zygotic --- Embryogenesis --- Embryogeny --- Embryological development --- Embryonic development --- Zoology --- Zygote development --- Zygotes --- Zygotic development --- Zygotic embryogenesis --- Developmental biology --- Morphology (Animals) --- Embryos --- Reproduction --- Balance of nature --- Biology --- Bionomics --- Ecological processes --- Ecological science --- Ecological sciences --- Environment --- Environmental biology --- Oecology --- Environmental sciences --- Population biology --- Ecology --- Nature conservation --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Chemistry --- Medical sciences --- Animal physiology --- Anatomy --- Fish --- Pisces --- Aquatic animals --- Vertebrates --- Fisheries --- Fishing --- Ichthyology --- Embryology --- Development --- Composition --- Physiology --- Aqüicultura --- Fisiologia animal --- Reproducció --- Peixos --- Fauna aquàtica --- Vertebrats --- Àgnats --- Condrictis --- Osteïctis --- Peixos d'aigua dolça --- Peixos d'aquari --- Peixos exòtics --- Peixos marins --- Peixos plans --- Piscicultura --- Ictiologia --- Indústria pesquera --- Peix --- Pesca --- Reproducció animal --- Reproducció sexual --- Biologia --- Vida (Biologia) --- Cèl·lules --- Embaràs --- Fecunditat --- Fetus --- Generació espontània --- Ovulació --- Part --- Partenogènesi --- Reproducció asexual --- Reproducció assistida --- Reproducció de les plantes --- Reproducció humana --- Aparell genital --- Embriologia --- Sexualitat --- Fisiologia --- Producció animal --- Agricultura --- Aqüicultura marina