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Plant cells and tissues --- Biotechnology --- Plant cytochemistry --- Technique

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With the 'post genomics' era comes an increasing demand for the techniques of cell biology, critical to interpreting the function and location of the cell's myriad proteins and macromolecules. In response, this second edition of Plant Cell Biology balances established techniques, including classical histochemistry and electron microscopy, with new developments in the field. The book covers a substantial range of methods for working on living cells, including the application of fluorescent probes, cytometry, expression systems, the use of green fluorescent protein, micromanipulation and electrophysiological techniques. Also featured are chapters on macromolecular location procedures involving immunocytochemistry and in situ hybridisation, and the book concludes with a range of biochemical techniques for the isolation of cytoplasmic organelles. The book provides advanced students, postgraduates and researchers in the plant sciences with an invaluably comprehensive guide to the ever-growing field of plant cell biology.

Botanical microscopy --- Plant cytochemistry --- Plant cells and tissues. --- Technique. --- Plant cells and tissues

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Plant cell biologists seek to characterize the principles underlying complex phenomena such as growth and differentiation and to define the ways in which plant cells respond to external and internal stimuli. This book discusses established techniques and presents some exciting advances that will have a major impact on the field in the future. The book begins with a detailed discussion of methods and protocols for viewing, staining, and localizing cell components. Subsequent chapters cover topics such as localizing specific nucleic acid sequences and proteins, protoplast research, wall analysis, plant cytoskeleton research, isolation and use of intact chloroplasts and thylakoids, and the measurement of ions and solutes within plant cells. Plant Cell Biology: A Practical Approach provides both newcomers and experienced researchers with a comprehensive practical guide to the subject.

Botanical microscopy --- Plant cells and tissues. --- Plant cytochemistry --- Plant and Crop Sciences. Botany --- Technique. --- Plant Cell Biology --- Plant Cell Biology. --- Plant cells and tissues --- Technique --- Botanical microscopy - Technique. --- Plant cytochemistry - Technique.

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Plant histochemistry --- Plant cytochemistry --- Methodology --- Methodology. --- SOLUTES --- LOCALIZATION --- IONS --- QUANTITATIVE ANALYSIS --- FLUORESCENT DYES --- VITAL DYES --- CARBOHYDRATES --- LIPIDS --- ENZYMIC REACTIONS --- PROTEINS --- NUCLEIC ACIDS --- TISSUES --- PREPARATION --- LABORATORY MANUALS --- CYTOCHEMISTRY --- PLANTS --- HISTOCHEMISTRY

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Phytochemistry. Phytobiochemistry --- Biological microscopy --- Botanical microscopy --- Plant cytochemistry. --- Plant anatomy. --- Electron microscopy --- Plant cells and tissues. --- Plant anatomy --- Plant cells and tissues --- Plant cytochemistry --- Technique. --- Technique --- 576.3 --- 581.1 --- General cytology. The cell as a biological system. General plan, chemical composition, physicochemical and molecular properties --- Plant physiology --- 581.1 Plant physiology --- 576.3 General cytology. The cell as a biological system. General plan, chemical composition, physicochemical and molecular properties --- Electron microscopy. --- Laboratories. --- Autoradiography --- Cytochemistry --- Enzymes --- Freeze-etching --- Glycoproteins --- Immunoelectron microscopy --- Ions --- Lectins --- Methods --- Plant cells --- Preparation --- Probes --- Proteins --- Scanning --- Plants --- Ultrastructure --- X-ray spectroscopy