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This book contains all the necessary information and advice for anyone wishing to obtain electron micrographs showing the most accurate ultrastructural detail in thin sections of any type of biological specimen.The guidelines for the choice of preparative methods are based on an extensive survey of current laboratory practice. For the first time, in a textbook of this kind, the molecular events occurring during fixation and embedding are analysed in detail. The reasons for choosing particular specimen preparation methods are explained and guidance is given on how to modify established techniques to suit individual requirements.All the practical methods advocated are clearly described, with accompanying tables and the results obtainable are illustrated with many electron micrographs.Portland Press Series: Practical Methods in Electron Microscopy, Volume 17, Audrey M. Glauert, EditorOriginally published in 1999.The Princeton Legacy Library uses the latest print-on-demand technology to again make available previously out-of-print books from the distinguished backlist of Princeton University Press. These editions preserve the original texts of these important books while presenting them in durable paperback and hardcover editions. The goal of the Princeton Legacy Library is to vastly increase access to the rich scholarly heritage found in the thousands of books published by Princeton University Press since its founding in 1905.

Mounting of microscope specimens. --- Transmission electron microscopy --- Electron microscopy --- Microscope specimens, Mounting of --- Microtechnique --- Technique. --- Acetonitrile (data page). --- Acrylic resin. --- Aldehyde. --- Animal testing. --- Antibody. --- Antigen-antibody interaction. --- Aqueous solution. --- Araldite. --- Autolysis (biology). --- Biochemist. --- Biological activity. --- Biological specimen. --- Biologist. --- Biology. --- Biopsy. --- Carbohydrazide. --- Catalysis. --- Cathode ray. --- Cell (biology). --- Cell culture. --- Cell membrane. --- Chemical formula. --- Chemical polarity. --- Chemistry. --- Chloroplast. --- Cryogenics. --- Curing (chemistry). --- Cyanide. --- Cytochemistry. --- Cytochrome c oxidase. --- Cytoplasm. --- Denaturation (biochemistry). --- Division (military). --- Electromagnetic radiation. --- Electron energy loss spectroscopy. --- Electron microscope. --- Electron tomography. --- Embedding. --- Enzyme catalysis. --- Epoxy. --- Erythrocytes. --- Ethanol. --- Ethylene glycol (data page). --- Ethylene glycol dimethacrylate. --- Ethylene glycol. --- Extraction (chemistry). --- Fixation (histology). --- Fixative (drawing). --- Formaldehyde. --- Formic acid (data page). --- Glutaraldehyde. --- Laboratory. --- Lipid. --- Macrophage (ecology). --- Micrograph. --- Microscope slide. --- Microscopy Society of America. --- Microscopy. --- Mixture. --- Moiety (chemistry). --- Mold. --- Molecular mass. --- Molecular sieve. --- Myoepithelial cell. --- Nuclear warfare. --- Nuclear weapon. --- Nuclear weapons delivery. --- Nucleic acid. --- Organic acid anhydride. --- Organic peroxide. --- Osmium tetroxide. --- Perfusion. --- Phosphate. --- Polymer. --- Polymerization. --- Polypropylene glycol. --- Propylene oxide. --- Protein structure. --- Protein. --- Radical (chemistry). --- Recrystallization (chemistry). --- Recrystallization (metallurgy). --- Resin. --- Ribosome. --- Room temperature. --- Scanning electron microscope. --- Solution. --- Solvent. --- Staining. --- Sterilization (microbiology). --- Tetrahydrofuran (data page). --- Tissue culture. --- Transmission electron microscopy. --- Triethylene glycol. --- Ultrastructure. --- Ultraviolet germicidal irradiation. --- Uranyl acetate. --- Vial. --- Viscosity. --- X-ray photoelectron spectroscopy.

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Nitric oxide has proven to be a molecule with wide biological significance. It is involved in myriad actions which range from physiology to pathophysiology. One of the fundamental questions in relation to its biological relevance concerns the paradoxical nature of some of its actions. For example, there is a whole range of effects related to cytoprotection, cell proliferation, differentiation, and cell death. The way in which nitric oxide becomes involved in pathophysiology is slowly being elucidated. This volume contains a summary of the discussions that took place at the Second International Paraelios Symposium on Nitric Oxide research, and will make a significant contribution to this very exciting subject.Originally published in 1998.The Princeton Legacy Library uses the latest print-on-demand technology to again make available previously out-of-print books from the distinguished backlist of Princeton University Press. These editions preserve the original texts of these important books while presenting them in durable paperback and hardcover editions. The goal of the Princeton Legacy Library is to vastly increase access to the rich scholarly heritage found in the thousands of books published by Princeton University Press since its founding in 1905.

Nitric oxide --- Cell proliferation --- Cell differentiation --- Apoptosis --- Physiological effect --- Pathophysiology --- Molecular aspects --- 7-Chlorokynurenic acid. --- 7-Nitroindazole. --- AMPA receptor. --- AMPA. --- Aconitase. --- Activation. --- Alkaline phosphatase. --- Antioxidant. --- Apoptosis. --- Arginine. --- Aspartic acid. --- Bcl-2. --- Botulinum toxin. --- Carcinogenesis. --- Cell death. --- Cellular respiration. --- Chloral hydrate. --- Cyanide. --- Cyclooxygenase. --- Cyclothiazide. --- Cytochrome c oxidase. --- Cytostasis. --- Cytotoxicity. --- Dialysis. --- EGTA (chemical). --- Electron transport chain. --- Enantiomer. --- Endonuclease. --- Endothelial NOS. --- Ethylenediaminetetraacetic acid. --- Excitotoxicity. --- Exocytosis. --- Gliotoxin. --- Glomerulonephritis. --- Glutamic acid. --- Glutathione. --- Glyceraldehyde 3-phosphate dehydrogenase. --- Glycerol. --- Glycine. --- Hydrogen peroxide. --- IC50. --- Immortalised cell line. --- Immunoassay. --- Immunohistochemistry. --- Interferon. --- L1210 cells. --- Lipid peroxidation. --- Lipopolysaccharide. --- Lipoxygenase. --- Lysis. --- Macrophage. --- Methemoglobin. --- Mitochondrion. --- N-Methyl-D-aspartic acid. --- NMDA receptor. --- Necrosis. --- Nitration. --- Nitric oxide. --- Nitrite. --- Nitrosamine. --- Nitrosation. --- Nitroso. --- Nitrosylation. --- Nitrous oxide. --- Nitrovasodilator. --- Nocodazole. --- Norepinephrine. --- Oxidative stress. --- Oxide. --- Oxypurinol. --- P53. --- Penicillamine. --- Peroxide. --- Peroxynitrite. --- Phosphodiesterase inhibitor. --- Phosphodiesterase. --- Phospholipid. --- Pro-oxidant. --- Propidium iodide. --- Prostacyclin. --- Protonation. --- Pyruvic acid. --- Radical (chemistry). --- Reactive nitrogen species. --- Reuptake. --- S-Nitrosoglutathione. --- S-Nitrosylation. --- Sodium nitrite. --- Sodium nitroprusside. --- Somatostatin. --- Superoxide dismutase. --- Superoxide. --- T cell. --- TNF inhibitor. --- Tetanospasmin. --- Thromboxane A2. --- Transfection. --- Transition metal. --- Trifluoperazine. --- Xanthine oxidase.