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This volume explores the latest synthetic procedures for producing receptor-specific retinoids and rexinoids, molecular biology methods, and new technologies to demonstrate the therapeutic activities of molecules. The chapters in this book cover topics such as lentiviral delivery of shRNA constructs into acute promyelocytic leukemia cells for ATRA induced differentiation and autophagy; methods to analyze RAR signaling in colorectal cancer cells; differentiation of primary myoblasts by using RXR agonist; methodology for analyzing effects of retinoid treatment on nervous system development and larval swimming behavior; and protocols for assessment of autophagic flux in ATRA treated 2D and 3D breast cancer cultures. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Retinoid and Rexinoid Signaling: Methods and Protocols is a valuable resources for graduate students, postdoctoral fellows, and principal investigators who are interested in further exploring the signaling mechanisms of these molecules in their specific preclinical models.

Biochemistry. --- Oncology. --- Biochemistry, general. --- Cancer Research. --- Tumors --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition --- Retinoides --- Transducció de senyal cel·lular --- Transducció de la informació cel·lular --- Regulació cel·lular --- Missatgers secundaris (Bioquímica) --- Carotenoides --- Vitamines liposolubles

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This detailed book expands upon the previous edition with a collection of methods for those performing experimental work on small GTPases of the Rho family. Split into four sections, the volume explores computational modeling and imaging procedures, biochemical methods related to post-translational modifications of Rho GTPases as well as some high throughput methods, functional assays that allow for monitoring the consequences of manipulating Rho GTPases in a variety of cell types and cell biology processes, and techniques specifically designed for studies in selected non-mammalian model organisms (zebrafish, social ameba, plants and algae). Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on trouble shooting and avoiding known pitfalls. Authoritative and up-to-date, Rho GTPases: Methods and Protocols, Second Edition constitutes an invaluable tool for all those with an interest in this remarkable family of signaling proteins.

Biochemistry. --- Protein Science. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition --- GTPases Rho --- Transducció de senyal cel·lular --- Manuals de laboratori --- Tècniques de laboratori --- Transducció de la informació cel·lular --- Regulació cel·lular --- Missatgers secundaris (Bioquímica) --- Família GTPasa Rho --- GTPases de la família Rho --- Proteïnes d'unió al GTP Rho --- Proteïnes G Rho --- Proteïnes GTP Rho --- Rho GTPases --- Rho (Enzims) --- Rho (Proteïnes)

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During recent years, research has greatly expanded our understanding of the sophisticated molecular network of responses which enable plants to develop, survive and propagate under a wide range of conditions. In Plant Signal Transduction: Methods and Protocols, an international panel of experts provide well-established methods vital to analyzing plant signal transduction on the molecular level. Featuring experimental procedures on several of the most popular model organisms, the volume focuses on in planta analyses and the proteins involved in signal transduction in order to aid with the establishment of laboratory techniques or the modification of the protocols for other plants. As part of the highly successful Methods in Molecular Biology™ series, the chapters include brief introductions to the subject, lists of necessary materials, readily reproducible laboratory protocols, and tips on trouble-shooting and avoiding known pitfalls. Comprehensive and cutting-edge, Plant Signal Transduction: Methods and Protocols will benefit plant scientists wishing to improve their experimental approaches and delve further into this exciting and important field of study.

Plant cellular signal transduction --- Plant molecular biology --- Growth and Development --- Signal Transduction --- Gene Expression Regulation, Plant --- Plant Physiological Phenomena --- Plants --- Gene Expression Regulation --- Physiological Processes --- Cell Physiological Processes --- Biochemical Processes --- Phenomena and Processes --- Eukaryota --- Chemical Processes --- Genetic Processes --- Physiological Phenomena --- Organisms --- Cell Physiological Phenomena --- Biochemical Phenomena --- Chemical Phenomena --- Genetic Phenomena --- Plant Physiology --- Botany --- Earth & Environmental Sciences --- Plant cellular signal transduction. --- Plant molecular biology. --- Transduction du signal cellulaire chez les plantes. --- Transduction du signal. --- Gene Expression Regulation, Plant. --- Plant Physiological Phenomena. --- Signal Transduction. --- growth & development. --- Genetic Concepts --- Genetic Phenomenon --- Genetic Process --- Concept, Genetic --- Concepts, Genetic --- Genetic Concept --- Phenomena, Genetic --- Phenomenon, Genetic --- Process, Genetic --- Processes, Genetic --- Chemical Phenomenon --- Chemical Process --- Physical Chemistry Phenomena --- Physical Chemistry Process --- Physicochemical Phenomenon --- Physicochemical Process --- Chemical Concepts --- Physical Chemistry Concepts --- Physical Chemistry Processes --- Physicochemical Concepts --- Physicochemical Phenomena --- Physicochemical Processes --- Chemical Concept --- Chemistry Process, Physical --- Chemistry Processes, Physical --- Concept, Chemical --- Concept, Physical Chemistry --- Concept, Physicochemical --- Concepts, Chemical --- Concepts, Physical Chemistry --- Concepts, Physicochemical --- Phenomena, Chemical --- Phenomena, Physical Chemistry --- Phenomena, Physicochemical --- Phenomenon, Chemical --- Phenomenon, Physicochemical --- Physical Chemistry Concept --- Physicochemical Concept --- Process, Chemical --- Process, Physical Chemistry --- Process, Physicochemical --- Processes, Chemical --- Processes, Physical Chemistry --- Processes, Physicochemical --- Biochemical Concepts --- Biochemical Phenomenon --- Biochemical Process --- Phenomena, Biochemical --- Biochemical Concept --- Concept, Biochemical --- Concepts, Biochemical --- Phenomenon, Biochemical --- Process, Biochemical --- Processes, Biochemical --- Cell Physiological Phenomenon --- Cell Physiological Process --- Physiology, Cell --- Cell Physiology --- Phenomena, Cell Physiological --- Phenomenon, Cell Physiological --- Physiological Process, Cell --- Physiological Processes, Cell --- Process, Cell Physiological --- Processes, Cell Physiological --- Cells --- Physiological Concepts --- Physiological Phenomenon --- Physiological Process --- Concept, Physiological --- Concepts, Physiological --- Phenomena, Physiological --- Phenomenas, Physiological --- Phenomenon, Physiological --- Physiological Concept --- Process, Physiological --- Processes, Physiological --- Eucarya --- Eukarya --- Eukaryotes --- Eukaryotas --- Eukaryote --- Expression Regulation, Gene --- Regulation, Gene Action --- Regulation, Gene Expression --- Gene Action Regulation --- Regulation of Gene Expression --- Plant --- Physiology, Plant --- Plant Physiologic Phenomena --- Plant Physiologic Phenomenon --- Plant Physiological Phenomenon --- Plant Physiological Process --- Plant Physiological Processes --- Phenomena, Plant Physiologic --- Phenomena, Plant Physiological --- Phenomenon, Plant Physiologic --- Phenomenon, Plant Physiological --- Phenomenons, Plant Physiological --- Physiologic Phenomena, Plant --- Physiologic Phenomenon, Plant --- Physiological Phenomena, Plant --- Physiological Phenomenon, Plant --- Physiological Phenomenons, Plant --- Physiological Process, Plant --- Physiological Processes, Plant --- Plant Physiological Phenomenons --- Process, Plant Physiological --- Processes, Plant Physiological --- Regulation, Gene Expression, Plant --- Plant Gene Expression Regulation --- Regulation of Gene Expression, Plant --- Receptor Mediated Signal Transduction --- Signal Transduction Pathways --- Signal Transduction Systems --- Receptor-Mediated Signal Transduction --- Signal Pathways --- Pathway, Signal --- Pathway, Signal Transduction --- Pathways, Signal --- Pathways, Signal Transduction --- Receptor-Mediated Signal Transductions --- Signal Pathway --- Signal Transduction Pathway --- Signal Transduction System --- Signal Transduction, Receptor-Mediated --- Signal Transductions --- Signal Transductions, Receptor-Mediated --- System, Signal Transduction --- Systems, Signal Transduction --- Transduction, Signal --- Transductions, Signal --- Development and Growth --- Molecular phytobiology --- Phytobiology, Molecular --- physiology --- Life sciences. --- Plant biochemistry. --- Plant science. --- Botany. --- Plant physiology. --- Life Sciences. --- Plant Physiology. --- Plant Sciences. --- Plant Biochemistry. --- Physiology --- Botanical science --- Phytobiology --- Phytography --- Phytology --- Plant biology --- Plant science --- Biology --- Natural history --- Phytochemistry --- Plant biochemistry --- Plant chemistry --- Biochemistry --- Phytochemicals --- Plant biochemical genetics --- Biosciences --- Sciences, Life --- Science --- Biochemistry. --- Biological chemistry --- Chemical composition of organisms --- Physiological chemistry --- Chemistry --- Medical sciences --- Composition --- Floristic botany --- Fisiologia vegetal --- Transducció de senyal cel·lular --- Fitoquímica --- Manuals de laboratori --- Tècniques de laboratori --- Bioquímica de les plantes --- Bioquímica vegetal --- Botànica química --- Química botànica --- Química de les plantes --- Química vegetal --- Bioquímica --- Hormones vegetals --- Pigments vegetals --- Proteïnes vegetals --- Biologia molecular vegetal --- Transducció de la informació cel·lular --- Regulació cel·lular --- Missatgers secundaris (Bioquímica) --- Botànica --- Absorció de l'aigua en les plantes --- Assimilació de les plantes --- Creixement (Plantes) --- Ecofisiologia vegetal --- Fecundació de les plantes --- Fisiologia postcollita --- Ritmes biològics en les plantes --- Floració --- Germinació --- Metabolisme de les plantes --- Moviments de les plantes --- Nutrició vegetal --- Reproducció de les plantes --- Respiració de les plantes --- Saprofitisme --- Tropisme