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Despite the success of earlier Neuromethads volumes, I was initially reluctant to edit a further volume because my own - search is concerned with nonneural tissues. I changed my mind for two simple reasons. First, though the sheer diversity of ext- cellular signal molecules is staggering, still more impressive is the remarkably small number of transmembrane signaling processes they recruit- their receptors either have integral ion channels or enzyme activities, or else they catalytically activate G proteins. Likewise, when we look to the final intracellular t- gets of these signaling pathways, they are stucturally diverse, but again there are common themes: the response may either be the gating of an ion channel, or else the phosphorylation of a target protein. Such conservation of signaling mechanisms is both impressive and convenient, and provides my justification for asking authors with interests in diverse tissues to contribute their methodological expertise to this volume. Second, I think it would be difficult to overestimate the extent to which our understanding of intracellular signaling has been transformed by new and improved methodology. Thus, simple methods for measuring inositol phosphates have revealed the profound and widespread importance of the ph- phoinositide pathways, the techniques of contemporary - lecular biology have provided unrivaled opportunities to relate structure and function, and the complex spatial and t- poral characteristics of intracellular signaling pathways were barely imaginable before the introduction of fluorescent indi- tors and single cell-imaging technology.

Neurochemistry --- Second messengers (Biochemistry) --- Second Messenger Systems. --- Research --- Methodology. --- Neurosciences. --- Neural sciences --- Neurological sciences --- Neuroscience --- Medical sciences --- Nervous system

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Essential fatty acids --- Physiological effect --- Congresses --- Neurochemistry --- Second messengers (Biochemistry) --- Cellular signal transduction --- Essential fatty acids - Physiological effect - Congresses. --- Neurochemistry - Congresses. --- Second messengers (Biochemistry) - Congresses. --- Cellular signal transduction - Congresses. --- FATTY ACIDS, ESSENTIAL --- SECOND MESSENGER SYSTEMS --- PHYSIOLOGY

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In Phospholipid Signaling Protocols, state-of-the-art techniques for monitoring the major lipid and phospholipid-derived second messenger pathways to identify and quantify pathway activation are detailed by experts intimately experienced in their use. The assays described cover all the major phospholipases (C, D, A2), as well as sphingomyelinase and its associated metabolites. Additional protocols are provided for the assay of phosphoinositide 3-, 4-, and 5-kinase and sphingosine kinase activity, and for the quantification, separation, and rigorous identification of phospholipids, diacylglycerol, and sphingolipids, as well as their metabolites, including phosphoinositols, choline metabolites, and fatty acid metabolites. In addition, there is extensive information on the extraction, size separation, detection, and quantification of cellular signaling proteins and corresponding mRNA, as well as a description of their localization by immunohistochemistry and immunocytochemistry. Phospholipid Signaling Protocols offers a wide-ranging collection of cutting-edge techniques for the study of signal transduction through phospholipid intermediates and their metabolites. The book is an indispensable reference for both the newcomer and the experienced researcher seeking to expand knowledge of these critical pathways, and strongly complements its companion volumes-R.A.J. Challiss' Receptor Signal Transduction Protocols, D. Bar-Sagi's, Transmembrane Signaling Protocols, and D. A. Kendall and S.J. Hill's Signal Transduction Protocols-in building a unique library of tried-and-tested protocols relating to the ever expanding signal transduction field.

Cellular signal transduction --- Membrane lipids --- Phospholipids --- Second messengers (Biochemistry) --- Research --- Methodology. --- Second messenger systems (Biochemistry) --- Secondary messengers (Biochemistry) --- Phosphatides --- Phosphates --- Membranes (Biology) --- Lipids --- Cellular information transduction --- Information transduction, Cellular --- Signal transduction, Cellular --- Bioenergetics --- Cellular control mechanisms --- Information theory in biology --- Biochemistry. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition

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The book offers an easy introduction to fast growing research areas in evolution of species, population genetics, ecological models, and population dynamics. The first two chapters review the concept and methodologies of phylogenetic trees; computational schemes and illustrations are given, including applications such as tracing the origin of SARS and influenza. The third chapter introduces the reader to ecological models, including predator-prey models. This chapter includes and introduction to reaction-diffusion equations, which are used to analyze the ecological models. The next chapter reviews a broad range of ongoing research in population dynamics, including evolution of dispersal models; it also features interesting mathematical theorems and lists open problems. The final chapter deals with gene frequencies under the action of migration and selection. The book is addressed to readers at the level of grad students and researchers. A background in PDEs is provided.

51-7 --- 51:37 --- 51:37 Mathematics-:-Opvoeding en onderwijs --(algemeen) --- Mathematics-:-Opvoeding en onderwijs --(algemeen) --- 51-7 Mathematical studies and methods in other sciences. Scientific mathematics. Actuarial mathematics. Biometrics. Econometrics etc. --- Mathematical studies and methods in other sciences. Scientific mathematics. Actuarial mathematics. Biometrics. Econometrics etc. --- Neurosciences informatiques --- Differential Equations. --- Mathematical Biology in General. --- Ordinary Differential Equations. --- Computational Mathematics and Numerical Analysis. --- Computer Appl. in Life Sciences. --- Models, Neurological --- Nerve Net --- Mathematical Modeling and Industrial Mathematics. --- Muscle Contraction --- Musculoskeletal Physiological Processes --- Musculoskeletal Physiological Phenomena --- Experimental Model --- Experimental Models --- Mathematical Model --- Model, Experimental --- Models (Theoretical) --- Models, Experimental --- Models, Theoretic --- Theoretical Study --- Mathematical Models --- Model (Theoretical) --- Model, Mathematical --- Model, Theoretical --- Studies, Theoretical --- Study, Theoretical --- Theoretical Model --- Theoretical Models --- Theoretical Studies --- Nervous Systems --- System, Nervous --- Systems, Nervous --- Computer mathematics --- Metabolic Phenomenon --- Metabolic Process --- Metabolism Concepts --- Metabolism Phenomena --- Process, Metabolic --- Processes, Metabolic --- Anabolism --- Catabolism --- Metabolic Concepts --- Metabolic Processes --- Concept, Metabolic --- Concept, Metabolism --- Concepts, Metabolic --- Concepts, Metabolism --- Metabolic Concept --- Metabolism Concept --- Phenomena, Metabolic --- Phenomena, Metabolism --- Phenomenon, Metabolic --- Musculoskeletal Physiologic Process --- Musculoskeletal Physiological Concepts --- Musculoskeletal Physiological Phenomenon --- Physiology, Musculoskeletal --- Musculoskeletal Physiologic Processes --- Musculoskeletal Physiological Process --- Musculoskeletal Physiology --- Concept, Musculoskeletal Physiological --- Concepts, Musculoskeletal Physiological --- Musculoskeletal Physiological Concept --- Phenomena, Musculoskeletal Physiological --- Phenomenon, Musculoskeletal Physiological --- Physiologic Process, Musculoskeletal --- Physiologic Processes, Musculoskeletal --- Process, Musculoskeletal Physiologic --- Process, Musculoskeletal Physiological --- Processes, Musculoskeletal Physiologic --- Processes, Musculoskeletal Physiological --- Active Ion Transport --- Facilitated Ion Transport --- Passive Ion Transport --- Antiport --- Ion Cotransport --- Ion Exchange, Intracellular --- Symport --- Uniport --- Cotransport, Ion --- Exchange, Intracellular Ion --- Intracellular Ion Exchange --- Ion Transport, Active --- Ion Transport, Facilitated --- Ion Transport, Passive --- Transport, Active Ion --- Transport, Ion --- Second Messengers --- Intracellular Second Messengers --- Intracellular Second Messenger --- Messenger, Second --- Messengers, Intracellular Second --- Messengers, Second --- Second Messenger --- Second Messenger System --- Second Messenger, Intracellular --- Second Messengers, Intracellular --- System, Second Messenger --- Systems, Second Messenger --- Calcium Puffs --- Calcium Sparks --- Calcium Spikes --- Calcium Oscillations --- Calcium Waves --- Calcium Oscillation --- Calcium Puff --- Calcium Signalings --- Calcium Spark --- Calcium Spike --- Calcium Wave --- Oscillation, Calcium --- Oscillations, Calcium --- Puff, Calcium --- Puffs, Calcium --- Signaling, Calcium --- Signalings, Calcium --- Spark, Calcium --- Sparks, Calcium --- Spike, Calcium --- Spikes, Calcium --- Wave, Calcium --- Waves, Calcium --- Muscular Contraction --- Inotropism --- Contraction, Muscle --- Contraction, Muscular --- Contractions, Muscle --- Contractions, Muscular --- Inotropisms --- Muscle Contractions --- Muscular Contractions --- Calculus of Variations and Optimal Control; Optimization. --- Physiological, Cellular and Medical Topics. --- Cell Cycle --- Processes, Cell Growth --- Cell Division Cycle --- Cell Cycles --- Cell Division Cycles --- Cycle, Cell --- Cycle, Cell Division --- Cycles, Cell --- Cycles, Cell Division --- Division Cycle, Cell --- Division Cycles, Cell --- Cell Multiplication --- Cell Number Growth --- Cell Growth in Number --- Cellular Proliferation --- Growth, Cell Number --- Multiplication, Cell --- Number Growth, Cell --- Proliferation, Cell --- Proliferation, Cellular --- Mathematics. --- Cell biology. --- Mathematical models. --- Biomathematics. --- Mathematical and Computational Biology. --- Cell Biology. --- Differential equations. --- Partial differential equations. --- Calculus of variations. --- Partial Differential Equations. --- Bioinformatics. --- Computational biology. --- Neurobiology. --- Computer mathematics. --- Ecology --- Evolution (Biology) --- Phylogeny --- Population genetics --- Animal phylogeny --- Animals --- Phylogenetics --- Phylogeny (Zoology) --- Biology --- Mathematical models --- Mathematical studies and methods in other sciences. Scientific mathematics. Actuarial mathematics. Biometrics. Econometrics etc --- Math --- Science --- Mathematics --- Models, Mathematical --- Simulation methods --- Cell biology --- Cellular biology --- Cells --- Cytologists --- Neurosciences --- Discrete mathematics --- Electronic data processing --- Partial differential equations --- 517.91 Differential equations --- Differential equations --- Bioinformatics --- Bio-informatics --- Biological informatics --- Information science --- Computational biology --- Systems biology --- Data processing --- Isoperimetrical problems --- Variations, Calculus of --- Maxima and minima --- Cellular signal transduction. --- Calcium --- Physiological effect. --- Computational neuroscience. --- Differential equations, partial. --- Mathematical optimization. --- Physiology --- Computer science --- Data processing. --- Cytology. --- Animal physiology --- Anatomy --- Optimization (Mathematics) --- Optimization techniques --- Optimization theory --- Systems optimization --- Mathematical analysis --- Operations research --- System analysis --- Cell proliferation. --- Cell cycle. --- Cancer cells. --- Pathology, Cellular --- Mitotic cycle --- Nuclear cycle (Cytology) --- Biological rhythms --- Cell renewal --- Cellular proliferation --- Cell cycle --- Cell division --- Cell populations --- Growth --- Neural circuitry --- Auditory pathways. --- Cellular signal transduction --- Physiological transport --- Physiological effect --- Metabolism --- Bioinformatics . --- Computational biology . --- Calcium - Physiological effect.

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The book considers signaling events from the zygote embryo through to the blastocyst with relevant data from embryonic stem (ES) cells, including dialogue with the extracellular environment and with the maternal tract during the implantation process. Application of the knowledge described to improve the success of human and animal assisted conception is considered where appropriate, but the focus is largely on fundamental rather than applied cell/molecular biology, as this is the area that has historically been neglected. While the general features of metabolism during preimplantation development are well established, especially in terms of nutrient requirements, uptake and fate, remarkably little is known about early embryo signaling events, intracellular or intercellular, between individual embryos in vitro or with the female reproductive tract in vivo. This contrasts with the wealth of information on cell signaling in somatic cells and tissues, as a glance at any textbook of biochemistry illustrates. This lack of information is such that our understanding of the molecular cell biology of early embryos -- a prerequisite to defining the mechanisms which regulate development at this critical stage of the life cycle -- is seriously incomplete. This volume is the first to address this issue by describing the current state of knowledge on cell signaling during mammalian early embryo development and highlighting priority areas for research.

Life Sciences. --- Embryology. --- Cell Biology. --- Reproductive Medicine. --- Life sciences. --- Cytology. --- Sciences de la vie --- Cytologie --- Embryologie --- Cells. --- Embryos. --- Mammals -- Reproduction. --- Embryology --- Mammals --- Embryonic stem cells --- Cellular signal transduction --- Cell interaction --- Embryonic and Fetal Development --- Cell Physiological Processes --- Biochemical Processes --- Embryonic Structures --- Stem Cells --- Second Messenger Systems --- Cell Physiological Phenomena --- Cells --- Anatomy --- Biochemical Phenomena --- Morphogenesis --- Reproduction --- Growth and Development --- Chemical Phenomena --- Phenomena and Processes --- Physiological Processes --- Reproductive Physiological Processes --- Reproductive Physiological Phenomena --- Physiological Phenomena --- Reproductive and Urinary Physiological Phenomena --- Cell Communication --- Embryonic Stem Cells --- Embryonic Induction --- Embryo Implantation --- Embryonic Development --- Calcium Signaling --- Signal Transduction --- Embryo, Mammalian --- Biology --- Health & Biological Sciences --- Biophysics --- Embryos --- Reproduction. --- Animal embryos --- Animals --- Reproductive medicine. --- Cell biology. --- Animal embryology --- Development, Embryological --- Development, Embryonic --- Development, Zygotic --- Embryogenesis --- Embryogeny --- Embryological development --- Embryonic development --- Zoology --- Zygote development --- Zygotes --- Zygotic development --- Zygotic embryogenesis --- Developmental biology --- Morphology (Animals) --- Cell biology --- Cellular biology --- Cytologists --- Human reproduction --- Human reproductive health --- Human reproductive medicine --- Reproductive medicine --- Health --- Biosciences --- Sciences, Life --- Science --- Development --- Health aspects --- Organisms --- Cytology