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The goal of this volume is to provide a comprehensive mechanistic and quantitative view of the processes that mediate or influence the quality control in translation. In addition to discussing processes with direct contribution to translation fidelity, such as aminoacylation of tRNAs and translation elongation itself, special attention is given to other processes with impact on quality control: detection and elimination of defective mRNAs, recycling and translation re-initiation, mRNA editing, and translational recoding through programmed frame-shifting. Provides a comprehensi
Genetic translation. --- Translation (Genetics) --- Genetic code --- Genetic regulation
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Molecular biology --- Genetic translation --- -Nucleic acids --- -Polynucleotides --- Biomolecules --- Translation (Genetics) --- Genetic code --- Genetic regulation --- Congresses --- Synthesis --- -Congresses --- Nucleic acids --- Congresses. --- Polynucleotides --- Synthesis&delete&
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Genetic transcription --- Genetic translation --- Viral genomes --- Viral genome --- Virus genomes --- Microbial genomes --- Translation (Genetics) --- Genetic code --- Genetic regulation --- Transcription (Genetics) --- General virology --- Molecular biology
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Genetic translation --- -Proteins --- -Proteids --- Biomolecules --- Polypeptides --- Proteomics --- Translation (Genetics) --- Genetic code --- Genetic regulation --- Laboratory manuals --- Synthesis --- -Laboratory manuals --- Proteins --- Traduction génétique --- Protéines --- Manuels de laboratoire --- Synthèse --- Proteids --- Synthesis&delete&
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Evolution. Phylogeny --- Molecular biology --- 530.161 --- #WSCH:AAS2 --- Principle of order in sequence of phenomena. Irreversibility. Heat death --- Molecular evolution. --- Genetic translation. --- 530.161 Principle of order in sequence of phenomena. Irreversibility. Heat death --- Molecular evolution --- Genetic translation --- Traduction génétique --- Evolution moléculaire --- Biochemical evolution --- Chemical evolution --- Evolution --- Life --- Translation (Genetics) --- Genetic code --- Genetic regulation --- Origin
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For over fifty years the Methods in Enzymology series has been the critically aclaimed laboratory standard and one of the most respected publications in the field of biochemistry. The highly relevant material makes it an essential publication for researchers in all fields of life and related sciences. This volume, the third of three on the topic of Translation Initiation includes articles written by leaders in the field.
Genetic regulation. --- Genetic translation. --- Proteins -- Synthesis. --- Protein Biosynthesis --- Gene Expression --- Peptide Biosynthesis --- Genetic Processes --- Biochemical Processes --- Metabolism --- Metabolic Phenomena --- Chemical Processes --- Genetic Phenomena --- Biochemical Phenomena --- Phenomena and Processes --- Chemical Phenomena --- Genetics --- Biology --- Health & Biological Sciences --- Gene expression --- Gene expression regulation --- Gene regulation --- Translation (Genetics) --- Regulation --- Biosynthesis --- Cellular control mechanisms --- Molecular genetics --- Genetic code --- Genetic regulation
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For over fifty years the Methods in Enzymology series has been the critically aclaimed laboratory standard and one of the most respected publications in the field of biochemistry. The highly relevant material makes it an essential publication for researchers in all fields of life and related sciences. This volume, the first of three on the topic of Translation Initiation includes articles written by leaders in the field.
Genetic regulation. --- Genetic translation. --- Protein Biosynthesis. --- Protein Biosynthesis --- Gene Expression --- Peptide Biosynthesis --- Genetic Processes --- Biochemical Processes --- Metabolism --- Chemical Processes --- Genetic Phenomena --- Metabolic Phenomena --- Biochemical Phenomena --- Phenomena and Processes --- Chemical Phenomena --- Gene expression --- Gene expression regulation --- Gene regulation --- Translation (Genetics) --- Regulation --- Biosynthesis --- Cellular control mechanisms --- Molecular genetics --- Genetic code --- Genetic regulation
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For over fifty years the Methods in Enzymology series has been the critically aclaimed laboratory standard and one of the most respected publications in the field of biochemistry. The highly relevant material makes it an essential publication for researchers in all fields of life and related sciences. This volume, the second of three on the topic of Translation Initiation includes articles written by leaders in the field.
Genetic regulation. --- Genetic translation. --- Protein. --- Proteins -- Synthesis. --- Protein Biosynthesis --- Peptide Biosynthesis --- Gene Expression --- Metabolism --- Genetic Processes --- Biochemical Processes --- Chemical Processes --- Genetic Phenomena --- Biochemical Phenomena --- Metabolic Phenomena --- Phenomena and Processes --- Chemical Phenomena --- Animal Biochemistry --- Human Anatomy & Physiology --- Health & Biological Sciences --- Gene expression --- Gene expression regulation --- Gene regulation --- Translation (Genetics) --- Regulation --- Biosynthesis --- Cellular control mechanisms --- Molecular genetics --- Genetic code --- Genetic regulation
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The majority of all proteins undergo posttranslational modifications that significantly alter their physical and chemical properties, including their folding and conformation distribution, their stability, and, consequently, their activity and function. In Posttranslational Modifications of Proteins: Tools for Functional Proteomics, Christoph Kannicht and a panel of highly experienced researchers describe readily reproducible methods for detecting and analyzing the most important of these modifications, particularly with regard to protein function, proteome research, and the characterization of pharmaceutical proteins. Among the methods presented are those for analyzing the assignment of disulfide bond sites in proteins, protein N-glycosylation and protein O-glycosylation, and oligosaccharides present at specific single glycosylation sites in a protein. Additional powerful techniques facilitate the analysis of glycosylphosphatidylinositols, lipid modifications, protein phosphorylation and sulfation, protein methylation and acetylation, a-amidation, g-glutamate, isoaspartate, and lysine hydroxylation. Comprehensive and state-of-the-art, Posttranslational Modifications of Proteins: Tools for Functional Proteomics serves as a highly practical guide for all investigators of protein structure-function relationships not only in chemical and pharmaceutical research, but also throughout the rapidly growing field of functional proteomics.
Post-translational modification --- Co-translational modification --- Cotranslational modification --- Modification, Post-translational --- Post-translation protein modification --- Post-translational protein modification --- Posttranslation protein modification --- Posttranslational modification --- Posttranslational protein modification --- Genetic translation --- Proteins --- Synthesis --- Genetic translation. --- Translation (Genetics) --- Life sciences. --- Cell biology. --- Life Sciences. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Biosciences --- Sciences, Life --- Science --- Genetic code --- Genetic regulation --- Cytology.
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The synthesis of proteins from 20 or so constituent amino acids according to a strictly defined code with an accuracy of better than 1 in 10,000 at most loca tions is arguably the most complex task performed by cells. Protein Synthesis collects together methods and protocols covering a range of different approaches towards understanding how the cellular machinery accomplishes this task and how these ftinctions might be harnessed by the biotechnology industry to generate novel and useful proteins. The era in which the components of the translational machinery were being catalogued is over. This volume gathers together protocols that focus on preserving and describing the dynamic function as closely as possible. The need to understand exactly how ribosomes are positioned on messages or where tRNA molecules, translation factors, or control proteins are bound, has been appreciated by many of the authors. Several chapters that explore the fidelity and processivity of translation reflect this belief. Moreover, the fundamental importance of rRNA at the heart of the ribosome is a strong theme in a number of the protocols. These articles include in vitro and in vivo systems from bacterial, fungal, plant, and animal systems. Overall, Protein Synthesis might be characterized by the novelty of the approaches employed to illuminate the inner workings of the protein synthetic machinery as well as by the inventiveness of the attempts to harness these reactions for biotechnological applications.
Genetic translation --- Protein Engineering --- Proteins --- Laboratory manuals. --- Methods. --- Synthesis --- Biosynthesis. --- Translation (Genetics) --- Life sciences. --- Biochemistry. --- Life Sciences. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Biosciences --- Sciences, Life --- Science --- Composition --- Genetic code --- Genetic regulation --- Proteids --- Biomolecules --- Polypeptides --- Proteomics
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