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Fidelity and quality control in gene expression
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ISBN: 9780123864970 0123864976 9786613434128 1283434121 0123864984 Year: 2012 Publisher: Amsterdam : Elsevier/Academic Press,

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The goal of this volume is to provide a comprehensive mechanistic and quantitative view of the processes that mediate or influence the quality control in translation. In addition to discussing processes with direct contribution to translation fidelity, such as aminoacylation of tRNAs and translation elongation itself, special attention is given to other processes with impact on quality control: detection and elimination of defective mRNAs, recycling and translation re-initiation, mRNA editing, and translational recoding through programmed frame-shifting. Provides a comprehensi

Translation of natural and synthetic polynucleotides
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ISBN: 044480045X Year: 1977 Publisher: Poznan University of agriculture in Poznan

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In vitro transcription and translation of viral genomes : colloque
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ISBN: 2855981158 Year: 1975 Publisher: Paris INSERM

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Protein expression : a practical approach
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ISBN: 0199636230 0199636249 Year: 1999 Volume: 202 Publisher: Oxford Oxford university press


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The hypercycle : a principle of natural self-organization
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ISBN: 3540092935 0387092935 3642672477 9780387092935 Year: 1979 Publisher: Berlin : Springer,

Translation initiation : cell biology, high-throughput methods, and chemical-based approaches
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ISBN: 0123739640 9786611059446 1281059447 0080553486 Year: 2007 Publisher: San Diego, Calif : Academic Press,

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For over fifty years the Methods in Enzymology series has been the critically aclaimed laboratory standard and one of the most respected publications in the field of biochemistry. The highly relevant material makes it an essential publication for researchers in all fields of life and related sciences. This volume, the third of three on the topic of Translation Initiation includes articles written by leaders in the field.

Translation initiation : extract systems and molecular genetics
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ISBN: 0123741912 9786611766221 1281766224 0080889662 9780123741912 Year: 2007 Publisher: San Diego, Calif : Academic Press,

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For over fifty years the Methods in Enzymology series has been the critically aclaimed laboratory standard and one of the most respected publications in the field of biochemistry. The highly relevant material makes it an essential publication for researchers in all fields of life and related sciences. This volume, the first of three on the topic of Translation Initiation includes articles written by leaders in the field.

Translation initiation : reconstituted systems and biophysical methods
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ISBN: 0123739691 9786611049683 1281049689 0080553184 Year: 2007 Publisher: Amsterdam ; London : Elsevier,

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For over fifty years the Methods in Enzymology series has been the critically aclaimed laboratory standard and one of the most respected publications in the field of biochemistry. The highly relevant material makes it an essential publication for researchers in all fields of life and related sciences. This volume, the second of three on the topic of Translation Initiation includes articles written by leaders in the field.

Posttranslational Modification of Proteins : Tools for Functional Proteomics
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ISBN: 0896036782 0585403163 9786610821433 1280821434 1592591817 9780896036789 Year: 2002 Volume: 194 Publisher: Totowa, NJ : Humana Press : Imprint: Humana,

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The majority of all proteins undergo posttranslational modifications that significantly alter their physical and chemical properties, including their folding and conformation distribution, their stability, and, consequently, their activity and function. In Posttranslational Modifications of Proteins: Tools for Functional Proteomics, Christoph Kannicht and a panel of highly experienced researchers describe readily reproducible methods for detecting and analyzing the most important of these modifications, particularly with regard to protein function, proteome research, and the characterization of pharmaceutical proteins. Among the methods presented are those for analyzing the assignment of disulfide bond sites in proteins, protein N-glycosylation and protein O-glycosylation, and oligosaccharides present at specific single glycosylation sites in a protein. Additional powerful techniques facilitate the analysis of glycosylphosphatidylinositols, lipid modifications, protein phosphorylation and sulfation, protein methylation and acetylation, a-amidation, g-glutamate, isoaspartate, and lysine hydroxylation. Comprehensive and state-of-the-art, Posttranslational Modifications of Proteins: Tools for Functional Proteomics serves as a highly practical guide for all investigators of protein structure-function relationships not only in chemical and pharmaceutical research, but also throughout the rapidly growing field of functional proteomics.

Protein Synthesis : Methods and Protocols
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ISBN: 089603397X 9786610836826 1280836822 1592595634 Year: 1998 Publisher: Totowa, NJ : Humana Press : Imprint: Humana,

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The synthesis of proteins from 20 or so constituent amino acids according to a strictly defined code with an accuracy of better than 1 in 10,000 at most loca­ tions is arguably the most complex task performed by cells. Protein Synthesis collects together methods and protocols covering a range of different approaches towards understanding how the cellular machinery accomplishes this task and how these ftinctions might be harnessed by the biotechnology industry to generate novel and useful proteins. The era in which the components of the translational machinery were being catalogued is over. This volume gathers together protocols that focus on preserving and describing the dynamic function as closely as possible. The need to understand exactly how ribosomes are positioned on messages or where tRNA molecules, translation factors, or control proteins are bound, has been appreciated by many of the authors. Several chapters that explore the fidelity and processivity of translation reflect this belief. Moreover, the fundamental importance of rRNA at the heart of the ribosome is a strong theme in a number of the protocols. These articles include in vitro and in vivo systems from bacterial, fungal, plant, and animal systems. Overall, Protein Synthesis might be characterized by the novelty of the approaches employed to illuminate the inner workings of the protein synthetic machinery as well as by the inventiveness of the attempts to harness these reactions for biotechnological applications.

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