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Step-by-step, practical guidance for the acquisition, manipulation, and use of cell sources for tissue engineering Tissue engineering is a multidisciplinary field incorporating the principles of biology, chemistry, engineering, and medicine to create biological substitutes of native tissues for scientific research or clinical use. Specific applications of this technology include studies of tissue development and function, investigating drug response, and tissue repair and replacement. This area is rapidly becoming one of the most promising treatment options for patients suffering from tissue failure. Written by leading experts in the field, Culture of Cells for Tissue Engineering offers step-by-step, practical guidance for the acquisition, manipulation, and use of cell sources for tissue engineering. It offers a unique focus on tissue engineering methods for cell sourcing and utilization, combining theoretical overviews and detailed procedures. Features of the text include: Easy-to-use format with a two-part organization Logically organized—part one discusses cell sourcing, preparation, and characterization and the second part examines specific engineered tissues Each chapter covers: structural and functional properties of tissues, methodological principles, culture, cell selection/expansion, cell modifications, cell seeding, tissue culture, analytical assays, and a detailed description of representative studies End-of-chapter features include useful listings of sources for reagents, materials, and supplies, with the contact details of the suppliers listed at the end of the book A section of elegant color plates to back up the figures in the chapters Culture of Cells for Tissue Engineering gives novice and seasoned researchers in tissue engineering an invaluable resource. In addition, the text is suitable for professionals in related research, particularly in those areas where cell and tissue culture is a new or emerging tool.
Histology. Cytology --- weefselcultuur --- cytologie --- histologie
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Histology. Cytology --- weefselcultuur --- cytologie --- histologie --- Acqui 2006 --- Tissue engineering --- Cell culture --- Tissue Engineering --- Cell Culture Techniques
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biologische technieken --- apoptose --- contaminatie --- tissue engineering --- in vitro --- Zoohistology. Zoocytology --- biotechnologie --- cytotoxiciteit --- weefselcultuur --- cytologie --- dierlijke cellenkweek --- Celkweek --- Cellenleer --- Cytologie (celleer) --- In vitro vermeerdering --- Animal biochemistry --- PXL-Tech 2016 --- opleiding agro- en biotechnologie --- celbiologie --- experimenten --- Biology
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Since publication of the previous edition of this benchmark text, numerous groundbreaking advances have occurred in stem cell research, cloning, tissue engineering, and in vitro toxicity testing. These and other developments have been incorporated into this fully revised and expanded Fifth Edition of Culture of Animal Cells. In addition, to answer the needs of the exponential increase in newcomers to cell culture, particularly in the biopharmaceutical industry, a completely new chapter on training in cell culture technology has been introduced. The most complete resource on the techniques, equipment, principles, and practices of animal cell culture, this text offers a complete background related to growth of animal cells in culture. Beginning with laboratory design, safety, validation and bioethics, then continuing with preparation of media, primary culture and cell lines, through to characterization and authentication, contamination, specialized techniques, and troubleshooting, the coverage includes: An allnew section of training exercises, separated into basic, intermediate, and advanced procedures, crossreferenced to the relevant protocols New coverage of stem cells, bioethics, validation, cloning, cell signaling, in vitro toxicity testing, and tissue engineering An expanded fullcolor atlas section, with images of primary culture, cell lines, subculture, differentiation, cancer cells and transformation, threedimensional culture, contamination, and specialized equipment Enhanced treatment of troubleshooting, with full crossreferencing to the relevant protocols and sections of text Fully updated references The clearest, most consistent presentation of stepbystep protocols available Numerous diagrams, photographs, tables, and charts Detailed and uptodate information on reagent preparation and sourcing of materials and equipment, including a fully updated list of suppliers and other resources with Web sites Indispensable for clinical and biopharmaceutical researchers and scientists, students, trainees, and technicians, this landmark text presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells.
Tissue culture --- Cell culture --- Cellules --- Laboratory manuals. --- Culture --- Manuels de laboratoire --- biologische technieken --- contaminatie --- antropogenetica --- aseptische technieken --- Zoohistology. Zoocytology --- celprocessen --- cytotoxiciteit --- weefselcultuur --- cytologie --- dierlijke cellenkweek --- Biological techniques --- histologie --- stamcellen --- webdesign --- klonen --- biogeneesmiddelen --- tumoren --- Tissue culture - Laboratory manuals --- Cell culture - Laboratory manuals --- Acqui 2006 --- Cells, cultured --- Bioethics --- methods --- physiology
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Cell culture. --- Tissue culture. --- Celcultuur --- Cellen--Cultuur --- Cellules [Culture de ] --- Cellules--Culture --- Culture cellulaire --- Culture de cellules --- Culture of cells --- Cultuur [Cel] --- Tissus [Culture des ] --- Weefselcultuur --- Tissue Culture Techniques. --- Organ Culture Techniques --- Coculture Techniques --- Cell Culture Techniques --- Culture Techniques --- Human Umbilical Vein Endothelial Cells --- Culture Technique, Tissue --- Culture Techniques, Tissue --- Tissue Culture Technique --- Cell culture --- Tissue culture --- Cells, Cultured --- Tissue Culture Techniques --- 57.086.83 --- 59.08 --- Cultured Cells --- Cell, Cultured --- Cultured Cell --- Tissues --- Cultures (Biology) --- Organ culture --- Cytology --- 59.08 Zoological techniques. Experimental methods. Equipment --- Zoological techniques. Experimental methods. Equipment --- 57.086.83 Methods and equipment for the cultivation of cells and tissues --- Methods and equipment for the cultivation of cells and tissues --- Cultures and culture media --- Technique --- Biological techniques --- Zoohistology. Zoocytology --- Cells, Cultured. --- Tissue Culture --- Tissus --- Cellules --- Culture
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