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This fully updated edition provides selected mouse genetic techniques and their application in modeling varieties of human diseases. The chapters are mainly focused on the generation of different transgenic mice to accomplish the manipulation of genes of interest, tracing cell lineages, and modeling human diseases. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Mouse Genetics: Methods and Protocols, Second Edition delivers fundamental techniques and protocols to geneticists, molecular biologists, cell and developmental biologists, students, and postdoctoral fellows working in the various disciplines of genetics, developmental biology, mouse genetics, and modeling human diseases.
Ratolins transgènics --- Ratolins genèticament modificats --- Animals transgènics --- Ratolins --- Biology—Technique. --- Medicine—Research. --- Biology—Research. --- Zoology. --- Biological Techniques. --- Biomedical Research. --- Biology --- Natural history --- Animals --- Ratolins (Animals de laboratori) --- Genètica animal --- Genètica --- Genètica veterinària --- Enginyeria genètica animal --- Millorament selectiu d'animals --- Ratolins com a animals de laboratori --- Animals de laboratori
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Enginyeria genètica --- Intervenció genètica --- Manipulació genètica --- Recombinació genètica --- ADN recombinant --- Clonatge --- Clonatge molecular --- Enginyeria de proteïnes --- Enginyeria genètica animal --- Enginyeria genètica vegetal --- Teràpia genètica --- Fecundació in vitro --- Organismes transgènics
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Animal biotechnology. --- Animals --- Domestic animals --- Livestock --- Livestock biotechnology --- Agricultural biotechnology --- Biotechnology --- Biotecnologia animal --- Ramaderia --- Cria d'animals domèstics --- Cria del bestiar --- Explotació del bestiar --- Sector ramader --- Producció animal --- Zoologia econòmica --- Bestiar oví --- Bestiar boví --- Deveses --- Pasturatge --- Ramaderia ecològica --- Agricultura --- Bestiar --- Biotecnologia agrícola --- Enginyeria genètica animal
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Aquaculture --- Animal biotechnology. --- Freshwater ecology. --- Biotechnology. --- Fresh water --- Fresh-water ecology --- Aquatic ecology --- Animals --- Domestic animals --- Livestock --- Livestock biotechnology --- Agricultural biotechnology --- Aquiculture --- Agriculture --- Ecology --- Biotechnology --- Ecologia d'aigua dolça --- Biotecnologia animal --- Ecologia d'aigües dolces --- Ecosistemes d'aigua dolça --- Biologia d'aigua dolça --- Ecologia aquàtica --- Ecologia dels llacs --- Ecologia fluvial --- Biotecnologia agrícola --- Enginyeria genètica animal
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This volume details generation of gene-edited cell lines and organisms as models for human diseases, pest control, and large animal welfare and production outcomes. Chapters guide readers through gene regulation, editing, screening of cell lines, genome editing, and an overview of the tools for efficient genome editing including; ZFNs, TALENs, and CRISPR. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step- by-step, readily reproducible protocols. Authoritative and cutting-edge, Applications of Genome Modulation and Editing aims to be a useful and practical guide for researchers to commence or advance their study in this field.
Genetics. --- Genetics and Genomics. --- Biology --- Embryology --- Mendel's law --- Adaptation (Biology) --- Breeding --- Chromosomes --- Heredity --- Mutation (Biology) --- Variation (Biology) --- Enginyeria genètica --- Regulació genètica --- Genomes --- Genoma --- Genoma humà --- Genòmica --- Biosíntesi --- Genètica molecular --- Inducció enzimàtica --- Expressió gènica --- Intervenció genètica --- Manipulació genètica --- Recombinació genètica --- ADN recombinant --- Clonatge --- Clonatge molecular --- Enginyeria de proteïnes --- Enginyeria genètica animal --- Enginyeria genètica vegetal --- Teràpia genètica --- Fecundació in vitro --- Organismes transgènics
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This detailed volume collects commonly used and cutting-edge methods to analyze alternative splicing, a key step in gene regulation. After an introduction of the alternative splicing mechanism and its targeting for therapeutic strategies, the book continues with techniques for analyzing alternative splicing profiles in complex biological systems, visualizing and localizing alternative spliced transcripts with cellular and sub-cellular resolution, probing regulators of alternative splicing, as well as assessing the functional consequences of alternative splicing. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Alternative Splicing: Methods and Protocols serves as an ideal guide for both RNA aficionados that want to implement novel approaches in their labs and novices undertaking alternative splicing projects.
Genetics. --- Genomics. --- Genetics and Genomics. --- Genome research --- Genomes --- Molecular genetics --- Biology --- Embryology --- Mendel's law --- Adaptation (Biology) --- Breeding --- Chromosomes --- Heredity --- Mutation (Biology) --- Variation (Biology) --- Research --- Enginyeria genètica --- Intervenció genètica --- Manipulació genètica --- Recombinació genètica --- ADN recombinant --- Clonatge --- Clonatge molecular --- Enginyeria de proteïnes --- Enginyeria genètica animal --- Enginyeria genètica vegetal --- Teràpia genètica --- Fecundació in vitro --- Organismes transgènics
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Aquaculture --- Animal biotechnology. --- Ecologia d'aigua dolça --- Biotecnologia animal --- Freshwater ecology. --- Biotechnology. --- Fresh water --- Fresh-water ecology --- Aquatic ecology --- Biotecnologia agrícola --- Enginyeria genètica animal --- Ecologia d'aigües dolces --- Ecosistemes d'aigua dolça --- Biologia d'aigua dolça --- Ecologia aquàtica --- Ecologia dels llacs --- Ecologia fluvial --- Animals --- Domestic animals --- Livestock --- Livestock biotechnology --- Agricultural biotechnology --- Aquiculture --- Agriculture --- Ecology --- Biotechnology
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Animal systematics, taxonomy, nomencl. --- Reptiles --- Animals --- Greek language --- Latin language --- Lexicology. --- Animaux --- Grec (Langue) --- Latin (Langue) --- Lexicologie --- Nomenclature --- Word formation. --- Formation des mots --- BPB1006 --- Zoologie --- 591.93 --- 480 --- Zoölogie --- Sciences Animals Ancient world --- Language Classical Philology --- Zoology --- Terminology --- History --- zooloogia --- zooloģija --- zoologi --- zoölogie --- zoology --- zoologija --- állattan --- zoología --- зоология --- zoologie --- зоологија --- zoologji --- żooloġija --- ζωολογία --- eläintiede --- zoológia --- zoologia --- genetica animale --- entomológia --- gjenetikë e kafshëve --- Insektenkunde --- ихтиологија --- tierische Genetik --- dzīvnieku ģenētika --- genética animal --- rovartan --- entomology --- генетика на животните --- læren om dyr --- génétique animale --- djurens anatomi --- entomologi --- entomologie --- ентомологија --- Entomologie --- εντομολογία --- entomología --- animal genetics --- entomologia --- наука за животните --- genetică animală --- entomologji --- genetika živočichů --- ορνιθολογία --- állatgenetika --- ornitologie --- ζωογενετική --- loomageneetika --- genetika životinja --- hyönteistiede --- entomologija --- gyvūnų genetika --- diergenetica --- entomoloģija --- ichtyologie --- entomoloogia --- орнитологија --- eläingenetiikka --- genetika živočíchov --- Tierkunde --- zó-eolaíocht --- Zoologie -- nomenclature --- Grec (langue) --- Latin (langue) --- Vocabulaire --- Sémantique
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This fully updated book collects numerous data mining techniques, reflecting the acceleration and diversity of the development of data-driven approaches to the life sciences. The first half of the volume examines genomics, particularly metagenomics and epigenomics, which promise to deepen our knowledge of genes and genomes, while the second half of the book emphasizes metabolism and the metabolome as well as relevant medicine-oriented subjects. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detail and expert implementation advice that is useful for getting optimal results. Authoritative and practical, Data Mining for Systems Biology: Methods and Protocols, Second Edition serves as an ideal resource for researchers of biology and relevant fields, such as medical, pharmaceutical, and agricultural sciences, as well as for the scientists and engineers who are working on developing data-driven techniques, such as databases, data sciences, data mining, visualization systems, and machine learning or artificial intelligence that now are central to the paradigm-altering discoveries being made with a higher frequency.
Bioinformatics. --- Bio-informatics --- Biological informatics --- Biology --- Information science --- Computational biology --- Systems biology --- Data processing --- Mineria de dades --- Genètica --- Bioinformàtica --- Informàtica biològica --- Ciències de la informació --- Biologia computacional --- Biologia --- Lleis de Mendel --- Al·lelomorfisme --- Cariotips --- Citogenètica --- Epigènesi --- Epigenètica --- Farmacogenètica --- Fenotip --- Genètica animal --- Genètica de poblacions --- Genètica de la conducta --- Genètica del desenvolupament --- Genètica evolutiva --- Genètica forense --- Genètica humana --- Genètica microbiana --- Genètica molecular --- Genètica quantitativa --- Genètica vegetal --- Herència citoplasmàtica --- Herència i medi --- Immunogenètica --- Neurogenètica --- Recursos genètics --- Selecció natural --- Sexualitat --- Adaptació (Biologia) --- Cromosomes --- Evolució --- Herència (Biologia) --- Millora de les espècies --- Mutació (Biologia) --- Nutrigenòmica --- Variació (Biologia) --- Prospecció de dades --- Cerca en bases de dades --- Arquitectura orientada a serveis (Informàtica) --- Extracció de dades de llocs web --- Mineria de web
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This updated second edition covers the molecular biology, genome engineering tools and comprehensive analysis techniques for Corynebacterium glutamicum. Aside from modern omics-based approaches, the authors also focus on cell physiology, including cell division, central carbon metabolic pathways, and the respiratory chain. Readers will learn how primary mechanisms like energy metabolism can be applied in processes like biorefinery. Newly added topics include cell envelope structures and aromatic compound metabolism in C. glutamicum. These chapters will be particularly useful for those interested in the microbial production of commodity chemicals, fuels, and proteins. Corynebacteriacea are already some of the most important industrial microorganisms. Understanding the cell physiology of C. glutamicum will help manufacturers to increase their product range and productivity through efficient metabolic engineering.
Microbiology. --- Biochemistry. --- Applied Microbiology. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Microbial biology --- Microorganisms --- Composition --- Corynebacterium glutamicum. --- Corynebacterium lilium --- Micrococcus glutamicus --- Corynebacterium --- Corynebacterium glutamicum --- Bacteris --- Enginyeria genètica --- Biologia molecular --- Biofísica molecular --- Bioquímica molecular --- Biofísica --- Bioquímica --- Histoquímica --- Biologia molecular vegetal --- Codi genètic --- Diagnòstic molecular --- Endocrinologia molecular --- Evolució molecular --- Farmacologia molecular --- Genètica molecular --- Glicòmica --- Metabolòmica --- Microbiologia molecular --- Neurobiologia molecular --- Patologia molecular --- Proteòmica --- Reconeixement molecular --- Biomolècules --- Intervenció genètica --- Manipulació genètica --- Recombinació genètica --- ADN recombinant --- Clonatge --- Clonatge molecular --- Enginyeria de proteïnes --- Enginyeria genètica animal --- Enginyeria genètica vegetal --- Teràpia genètica --- Fecundació in vitro --- Organismes transgènics --- Microbis --- Microorganismes --- Antígens bacterians --- Bacteris anaerobis --- Bacteris fotosintetitzadors --- Bacteris gramnegatius --- Bacteris nitrificants --- Bacteris patògens --- Lactobacils --- Listeria --- Virus
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