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Liver --- Liver Cirrhosis, Experimental --- Hepatocytes --- Liver Cirrhosis --- Histocytological Preparation Techniques --- drug effects --- drug therapy --- pathology --- pathology

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Central nervous system --- Vertebrates --- Vertebrates --- Central Nervous System --- Histocytological Preparation Techniques --- Vertebrates --- Histochemistry --- Technique --- Histology --- Nervous system --- anatomy & histology --- anatomy & histology

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Presenting an area of research that intersects with and integrates diverse disciplines, including genomics, epigenetics, proteomics, and cellular biology, among others, Formalin-Fixed Paraffin-Embedded Tissues: Methods and Protocols collects contributions from expert researchers in order to provide practical guidelines to this complex study. Compiled in order to provide researchers with up-to-date methodological information pertaining to the utilization of genomic, transcriptomic, and proteomic data in diagnosis, prognosis, and tailored therapy, the ultimate aim of this volume is to decipher diseases at a molecular level. Divided into multiple convenient chapters, this detailed book covers various techniques to construct and utilize tissue arrays, it also provides detailed protocols in immunohistochemistry, immunofluorescence, fluorescent and chromogenic in situ hybridization, and ultimately introduces protocols for FFPET microdissection and nucleic acids extraction for their utilization in advanced techniques such as microarray CGH, DNA methylation and pyrosequencing. The volume also discusses FFPET research from an ethical standpoint and concludes with a chapter on novel tissue fixative. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and accessible, Formalin-Fixed Paraffin-Embedded Tissues: Methods and Protocols serves as a practical guide for scientists of all backgrounds and aims to convey the appropriate sense of fascination associated with this vital field of research.

Human histology. Human cytology --- Chemical laboratory practice --- Tissues --- Tissue culture --- Diagnostic immunohistochemistry --- Tissue Embedding --- Genetic Techniques --- Microarray Analysis --- Culture Techniques --- Publication Formats --- Investigative Techniques --- Clinical Laboratory Techniques --- Histocytological Preparation Techniques --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Publication Characteristics --- Microchip Analytical Procedures --- Cytological Techniques --- Chemistry Techniques, Analytical --- Histological Techniques --- Gene Expression Profiling --- Laboratory Manuals --- Paraffin Embedding --- Tissue Array Analysis --- Methods --- Tissue Culture Techniques --- Human Anatomy & Physiology --- Biology --- Health & Biological Sciences --- Physiology --- Biophysics --- Analysis --- Embedding, Paraffin --- Gene Expression Monitoring --- Transcriptome Analysis --- Gene Expression Pattern Analysis --- Transcript Expression Analysis --- Transcriptome Profiling --- mRNA Differential Display --- Analyses, Transcript Expression --- Analyses, Transcriptome --- Analysis, Transcript Expression --- Analysis, Transcriptome --- Differential Display, mRNA --- Differential Displays, mRNA --- Display, mRNA Differential --- Displays, mRNA Differential --- Gene Expression Monitorings --- Gene Expression Profilings --- Monitoring, Gene Expression --- Monitorings, Gene Expression --- Profiling, Gene Expression --- Profiling, Transcriptome --- Profilings, Gene Expression --- Profilings, Transcriptome --- Transcript Expression Analyses --- Transcriptome Analyses --- Transcriptome Profilings --- mRNA Differential Displays --- Histologic Technic --- Histologic Technics --- Histologic Technique --- Histologic Techniques --- Histological Technics --- Technic, Histologic --- Technics, Histologic --- Technique, Histologic --- Techniques, Histologic --- Histological Technic --- Histological Technique --- Technic, Histological --- Technics, Histological --- Technique, Histological --- Techniques, Histological --- Histology --- Analytical Chemistry Methods --- Analytical Chemistry Techniques --- Analytical Chemistry Method --- Analytical Chemistry Technique --- Chemistry Method, Analytical --- Chemistry Methods, Analytical --- Chemistry Technique, Analytical --- Method, Analytical Chemistry --- Methods, Analytical Chemistry --- Technique, Analytical Chemistry --- Techniques, Analytical Chemistry --- Cytologic Technics --- Cytological Technic --- Cytological Technics --- Cytological Technique --- Technic, Cytological --- Technics, Cytological --- Technique, Cytological --- Techniques, Cytological --- Cytologic Technic --- Technic, Cytologic --- Technics, Cytologic --- Cell Biology --- Microchip Analysis --- Analyses, Microchip --- Analysis, Microchip --- Analytical Procedure, Microchip --- Analytical Procedures, Microchip --- Microchip Analytical Procedure --- Procedure, Microchip Analytical --- Procedures, Microchip Analytical --- Cytohistologic Preparation Technics --- Cytohistologic Preparation Technique --- Cytohistologic Preparation Techniques --- Cytohistological Preparation Technic --- Cytohistological Preparation Technics --- Cytohistological Preparation Technique --- Histocytologic Preparation Technic --- Histocytologic Preparation Technics --- Histocytologic Preparation Technique --- Histocytologic Preparation Techniques --- Histocytological Preparation Technic --- Histocytological Preparation Technics --- Histocytological Preparation Technique --- Preparation Technic, Cytohistologic --- Preparation Technic, Cytohistological --- Preparation Technic, Histocytologic --- Preparation Technic, Histocytological --- Preparation Technics, Cytohistologic --- Preparation Technics, Cytohistological --- Preparation Technics, Histocytologic --- Preparation Technics, Histocytological --- Preparation Technique, Cytohistologic --- Preparation Technique, Cytohistological --- Preparation Technique, Histocytologic --- Preparation Technique, Histocytological --- Preparation Techniques, Cytohistologic --- Preparation Techniques, Cytohistological --- Preparation Techniques, Histocytologic --- Preparation Techniques, Histocytological --- Technic, Cytohistologic Preparation --- Technic, Cytohistological Preparation --- Technic, Histocytologic Preparation --- Technic, Histocytological Preparation --- Technics, Cytohistologic Preparation --- Technics, Cytohistological Preparation --- Technics, Histocytologic Preparation --- Technics, Histocytological Preparation --- Technique, Cytohistologic Preparation --- Technique, Cytohistological Preparation --- Technique, Histocytologic Preparation --- Technique, Histocytological Preparation --- Techniques, Cytohistologic Preparation --- Techniques, Cytohistological Preparation --- Techniques, Histocytologic Preparation --- Techniques, Histocytological Preparation --- Cytohistological Preparation Techniques --- Cytohistologic Preparation Technic --- Diagnoses and Laboratory Examinations --- Diagnosis, Laboratory --- Laboratory Diagnosis --- Laboratory Examinations and Diagnoses --- Laboratory Techniques, Clinical --- Clinical Laboratory Technique --- Diagnoses, Laboratory --- Laboratory Diagnoses --- Laboratory Technique, Clinical --- Technique, Clinical Laboratory --- Techniques, Clinical Laboratory --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Culture Technique --- Technique, Culture --- Techniques, Culture --- Microarray Analytical Devices --- Microarray Microchips --- Nanoarray Analytical Devices --- Analysis, Microarray --- Analytical Device, Microarray --- Analytical Device, Nanoarray --- Analytical Devices, Microarray --- Analytical Devices, Nanoarray --- Device, Microarray Analytical --- Device, Nanoarray Analytical --- Devices, Microarray Analytical --- Devices, Nanoarray Analytical --- Microarray Analytical Device --- Microarray Microchip --- Microchip, Microarray --- Microchips, Microarray --- Nanoarray Analytical Device --- Genetic Technic --- Genetic Technics --- Genetic Technique --- Technic, Genetic --- Technics, Genetic --- Technique, Genetic --- Techniques, Genetic --- Genetics --- Diagnostic immunohistology --- Culture Technique, Tissue --- Culture Techniques, Tissue --- Tissue Culture Technique --- Methodological Studies --- Methodological Study --- Procedures --- Studies, Methodological --- Study, Methodological --- Method --- Procedure --- methods --- Cultures and culture media --- Cell Array Analysis --- Cell Microarray Analysis --- Tissue Microarray Analysis --- Analyses, Cell Array --- Analyses, Cell Microarray --- Analyses, Tissue Array --- Analyses, Tissue Microarray --- Analysis, Cell Array --- Analysis, Cell Microarray --- Analysis, Tissue Array --- Analysis, Tissue Microarray --- Array Analyses, Cell --- Array Analyses, Tissue --- Array Analysis, Cell --- Array Analysis, Tissue --- Cell Array Analyses --- Cell Microarray Analyses --- Microarray Analyses, Cell --- Microarray Analyses, Tissue --- Microarray Analysis, Cell --- Microarray Analysis, Tissue --- Tissue Array Analyses --- Tissue Microarray Analyses --- Embedding, Tissue --- Medicine. --- Pathology. --- Molecular Medicine. --- Disease (Pathology) --- Medical sciences --- Diseases --- Medicine --- Medicine, Preventive --- Health Workforce

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Frozen sections are performed while a patient is undergoing surgery as a basis for making an immediate diagnosis that will impact treatment decisions. Frozen section diagnosis is often a highly demanding situation for the pathologist who must render a diagnosis quickly for the patient and surgeon. The Frozen Section Library series will provide concise, user-friendly, site specific handbooks that are well illustrated and highlight the pitfalls, artifacts and differential diagnosis issues that arise in the hurried frozen section scenario. Omar Hameed, M.D., is an Assistant Professor, Department of Pathology, University of Alabama at Birmingham, Birmingham, Alabama, USA. Shi Wei, M.D.,Ph.D., is an Assistant Professor, Department of Pathology, University of Alabama, Birmingham, Alabama, USA. Gene P. Siegal, M.D., Ph.D., is a Robert W. Mowry Endowed Professor of Pathology & Director, Division of Anatomic Pathology; Executive Vice-Chair of Pathology - UAB Health System; Sr. Scientist, UAB Comprehensive Cancer Center & the Gene Therapy Center, Department of Pathology, University of Alabama at Birmingham, Birmingham, Alamaba, USA.   Gene P. Siegal, M.D., Ph.D., is a Robert W. Mowry Endowed Professor of Pathology & Director, Division of Anatomic Pathology; Executive Vice-Chair of Pathology - UAB Health System; Sr. Scientist, UAB Comprehensive Cancer Center & the Gene Therapy Center, Department of Pathology, University of Alabama at Birmingham, Birmingham, Alamaba, USA.   Gene P. Siegal, M.D., Ph.D., is a Robert W. Mowry Endowed Professor of Pathology & Director, Division of Anatomic Pathology; Executive Vice-Chair of Pathology - UAB Health System; Sr. Scientist, UAB Comprehensive Cancer Center & the Gene Therapy Center, Department of Pathology, University of Alabama at Birmingham, Birmingham, Alamaba, USA.  .

Bone regeneration. --- Bones -- Diseases. --- Bones. --- Cryoultramicrotomy --- Neoplasms by Site --- Bone Diseases --- Musculoskeletal Diseases --- Neoplasms --- Microtomy --- Histocytological Preparation Techniques --- Diseases --- Histological Techniques --- Cytological Techniques --- Clinical Laboratory Techniques --- Investigative Techniques --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Frozen Sections --- Bone Neoplasms --- Medicine --- Health & Biological Sciences --- Musculoskeletal System Diseases --- Pathology --- Bones --- Diseases. --- Osteology --- Medicine. --- Pathology. --- Medicine & Public Health. --- Musculoskeletal system --- Bone --- Skeleton --- Disease (Pathology) --- Medical sciences --- Medicine, Preventive

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Frozen sections are performed while a patient is undergoing surgery as a basis for making an immediate diagnosis that will impact treatment decisions. Frozen section diagnosis is often a highly demanding situation for the pathologist who must render a diagnosis quickly for the patient and surgeon. The Frozen Section Library series will provide concise, user-friendly, site specific handbooks that are well illustrated and highlight the pitfalls, artifacts and differential diagnosis issues that arise in the hurried frozen section scenario. Philip T. Cagle, MD, is Professor of Pathology, Weill Medical College of Cornell University, New York and Director of Pulmonary Pathology, The Methodist Hospital, Houston, Texas. Timothy Craig Allen, MD, JD, is the Chairman of the Department of Pathology, University of Texas Health Science Center at Tyler, Tyler, Texas.

Lungs -- Diseases -- Atlases. --- Lungs --- Serous Membrane --- Cryoultramicrotomy --- Respiratory System --- Membranes --- Microtomy --- Anatomy --- Pleura --- Frozen Sections --- Tissues --- Histocytological Preparation Techniques --- Cytological Techniques --- Histological Techniques --- Clinical Laboratory Techniques --- Investigative Techniques --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Medicine --- Health & Biological Sciences --- Pathology --- Respiratory System Diseases --- Diseases --- Frozen tissue sections --- Diseases. --- Frozen histological sections --- Frozen sections (Histology) --- Histological sections, Frozen --- Tissue sections, Frozen --- Medicine. --- Pathology. --- Medicine & Public Health. --- Cryopreservation of organs, tissues, etc. --- Cryotomy --- Preservation --- Disease (Pathology) --- Medical sciences --- Medicine, Preventive