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Book
Synthetic biology of yeasts : tools and applications
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ISBN: 3030896803 303089679X Year: 2022 Publisher: Cham, Switzerland : Springer,

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Yeast protocols : methods in cell and molecular biology
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ISBN: 9780896033191 0896033198 9781592595402 9786610836628 1280836628 1592595405 Year: 1996 Volume: 53 Publisher: Totowa Humana press

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Yeast Protocols intends to offer a selection of well-proven proto­ cols in cell and molecular biology, applicable to yeasts including, but certainly not exclusively aimed at, Saccharomyces cerevisiae. Saccharomyces cerevisiae and its very distant cousin, Schizosacc- romyces pombe, are of course now foremost model eukaryotic organisms, and the focus of wide-ranging experimental studies, especially those using molecular genetic techniques. Many of the lat­ ter, such as DNA sequencing and in vitro mutagenesis, are general DNA techniques, well covered in other volumes of Humana's Meth­ ods in Molecular Biology series, and elsewhere. The inclusion of a number of non-DNA techniques in this book is meant to reflect the resurgent interest in yeast cell biology sparked by the development of gene manipulation methods—for example, cellular localization of cloned gene products using microscopical techniques. The presentation of protocols follows the successful Methods in Molecular Biology series format, with a clear sequence of steps and extensive troubleshooting notes. It is our hope that these protocols will be useful not only to established members of the full-time research community, but also to the less experienced—first degree level and masters students undertaking project work, as well as PhD students starting their experimental programs; I am well aware that these young apprentice scientists are not always able to receive the supervision time that they, and indeed their supervisors, would like.


Book
Yeast Biotechnology: Diversity and Applications
Authors: ---
ISBN: 1402082916 9048178339 9786612236624 1282236628 1402082924 Year: 2009 Publisher: Dordrecht : Springer Netherlands : Imprint: Springer,

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I belie ve that the book would provide an overview of the recent developments in the domain of yeast research with some new ideas, which could serve as an inspiration and challenge for researchers in this field. Ne w Delhi Prof. Asis Datta Dec. 24, 2007 Former Vice-chancellor, JNU Director, NCPGR (New Delhi) Preface Yeasts are eukaryotic unicellular microfungi that are widely distributed in the natural environments. Although yeasts are not as ubiquitous as bacteria in the natural environments, they have been isolated from terrestrial, aquatic and atmospheric environments. Yeast communities have been found in association with plants, animals and insects. Several species of yeasts have also been isolated from specialized or extreme environments like those with low water potential (e. g. high sugar/salt concentrations), low temperature (e. g. yeasts isolated from Antarctica), and low oxygen availability (e. g. intestinal tracts of animals). Around 1500 species of yeasts belonging to over 100 genera have been described so far. It is estimated that only 1% of the extant yeasts on earth have been described till date. Therefore, global efforts are underway to recover new yeast species from a variety of normal and extreme environments. Yeasts play an important role in food chains, and carbon, nitrogen and sulphur cycles. Yeasts can be genetically manipulated by hybridization, mutation, rare m- ing, cytoduction, spheroplast fusion, single chromosomal transfer and transformation using recombinant technology. Yeasts (e. g.


Book
Saccharomyces
Authors: ---
ISBN: 1839687908 1839687894 Year: 2021 Publisher: London : IntechOpen,


Book
Biotechnological Applications of the Yeast Yarrowia lipolytica
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ISBN: 3319064371 3319064363 Year: 2014 Publisher: Cham : Springer International Publishing : Imprint: Springer,

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Due to its ability to degrade hydrophobic substrates highly efficiently, the non-conventional yeast Yarrowia lipolytica offers various applications in the food, feed, pharmaceutical, and fine chemistry industries, as well as in environmental protection contexts such as waste treatment and bioremediation. The yeast breaks down hydrophobic substrates such as n-alkanes, fatty acids, fats, and oils, yielding valuable biotechnological products such as organic acids, extracellular enzymes, aroma compounds, bioemulsifiers, polyols, single-cell proteins, and single-cell oils. Further, Y. lipolytica is an efficient platform for the production of various heterologous proteins; more than a hundred heterologous proteins – including those of viruses, bacteria and fungi, as well as plant, animal and human proteins – have already been produced in Y. lipolytica. Since its genome has been sequenced, it is now possible to use new recombinant technology and metabolic engineering in order to improve the metabolic pathways involved in the creation of desirable metabolites and products.


Book
Molecular Mechanisms in Yeast Carbon Metabolism
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ISBN: 3642550134 3642550126 Year: 2014 Publisher: Berlin, Heidelberg : Springer Berlin Heidelberg : Imprint: Springer,

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This book provides a comprehensive review of recent developments and achievements in the field of yeast carbon metabolism, from academic studies on gene expression to biotechnology-relevant aspects. Yeast is one of the most widely studied laboratory organisms and represents one of the most essential models for understanding how any eukaryote cell works. On the other hand, yeast fermentations have for millennia provided us with a variety of biotech products, like wine, beer, vitamins, and recently also with pharmaceutically active heterologous products and biofuels. A key biochemical activity in the yeast cell is the metabolism of carbon compounds, which provides energy for the whole cell, as well as precursors for any of the final fermentation products. A complex set of genes and regulatory pathways control the metabolism of carbon compounds, from nutrient sensing and signal transduction to transcription regulation and post-transcriptional events. Recent advances in comparative genomics and the development of post-genomic tools have provided further insights into the network of genes and enzymes, and into the molecular mechanisms which are responsible for a balanced metabolism of carbon compounds in the yeast cell, and which could be manipulated in the laboratory to increase the yield and quality of yeast biotech products.


Book
Yarrowia lipolytica : Biotechnological Applications
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ISBN: 3642385826 3642435556 3642385834 Year: 2013 Publisher: Berlin, Heidelberg : Springer Berlin Heidelberg : Imprint: Springer,

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Due to various special physiological features and a genome that greatly differs in structure, gene content and organization from other yeasts, Y. lipolytica is widely used as a host system. With its characteristics, such as the ability to grow on lipids or grease, to accumulate oil and the high capacity for secretion of proteases and lipases, the yeast is of great interest for biotechnological applications. The main topics covered in this Microbiology Monograph are: expression and secretion of heterologous proteins; acid and alkaline extracellular proteases; genetics, production, biochemical characterization and biotechnological application of lipases; production and secretion of several organic acids and flagrances; as well as the functional expression of P450 systems and its use in steroid biotransformation.


Book
Cold-adapted Yeasts : Biodiversity, Adaptation Strategies and Biotechnological Significance
Authors: ---
ISBN: 3642396801 364239681X Year: 2014 Publisher: Berlin, Heidelberg : Springer Berlin Heidelberg : Imprint: Springer,

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Yeasts are a versatile group of eukaryotic microorganisms, exhibiting heterogeneous nutritional profiles and an extraordinary ability to survive in a wide range of natural and man-associated ecosystems, including cold habitats. Cold-adapted yeasts inhabit numerous low-temperature environments where they are subjected to seasonal or permanent cold conditions. Hence, they have evolved a number of adaptation strategies with regard to growth and reproduction, metabolic activities, survival and protection. Due to their distinctive ability to thrive successfully at low and even subzero temperatures, cold-adapted yeasts are increasingly attracting attention in basic science and industry for their enormous biotechnological potential. This book presents our current understanding of the diversity and ecology of cold-adapted yeasts in worldwide cold ecosystems, their adaptation strategies, and their biotechnological significance. Special emphasis is placed on the exploitation of cold-adapted yeasts as a source of cold-active enzymes and biopolymers, as well as their benefits for food microbiology, bioremediation and biocontrol. Further, aspects of food biodeterioration are considered.


Book
Biotechnology of Yeasts and Filamentous Fungi
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ISBN: 331958829X 3319588281 Year: 2017 Publisher: Cham : Springer International Publishing : Imprint: Springer,

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This book provides a comprehensive overview on biotechnological applications of unicellular and multicellular fungi in a variety of industrial branches. Targeted genetic and metabolic engineering of fungi allows production of native and transgenic enzymes and proteins in industrial scales. Those most prominently find application in biorefineries for the production of value-added chemicals and biofuels, in the pharmaceutical industry as well as in biomedicine. Each chapter is dedicated to applications and potential beneficial use of particular strains of yeasts and filamentous fungi and their produced biomolecules. The book targets researchers from both academia and industry and graduate students working in microbial biotechnology.

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